Phenotypes associated with this allele

• Allele Symbol: Wasf1^tm1Jsco
• Allele Name: targeted mutation 1, John D Scott
• Allele ID: MGI:2451310
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wasf1^tm1Jsco/Wasf1^tm1Jsco	involves: 129X1/SvJ * C57BL/6	MGI:2451314

Genotype
MGI:2451314

hm1

• Allelic Composition: Wasf1^tm1Jsco/Wasf1^tm1Jsco
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality ( J:81968 )

• homozygous progeny are observed in lower numbers than expected from heterozygous intercrosses

growth/size/body

decreased body weight ( J:81968 )

• between 2 and 6 weeks, reduced body size of homozygotes compared to littermates is observed, despite no differences in daily food intake; by 7 weeks of age, mice weigh 90% of control littermates

behavior/neurological

abnormal object recognition memory ( J:81968 )

• mice are unable to distinguish between a novel and familiar object, indicating impaired nonspatial learning/memory

abnormal spatial learning ( J:81968 )

• homozygous mice show impaired spatial learning/memory in a hidden platform swim test

decreased anxiety-related response ( J:81968 )

• null mice spend more time in center of open field compared to controls; in elevated zero maze, homozygotes spend 41% of time in open areas compared to 12 or 14% for wild-type or heterozygous mice

impaired balance ( J:81968 )

• on balance beam, mutants travel 198 cm compared to 568 or 439 cm for wild-type or heterozygous littermates

impaired coordination ( J:81968 )

• mice fall off rotating rod with latency of ~43 seconds compared to 114 and 93 seconds for wild-type and heterozygous mice; mice cannot compensate for this with training

nervous system

abnormal neuron morphology ( J:117303 )

• growth cones of E18 hippocampal neurons appear more 'spiky' and elongated than wild-type growth cones; roundness index of mutant growth cones is 5.3 compared to wild-type score of 4.3

• in vivo, CA1 hipoocampal and cortical neurons in 10-week old mice show a reduction in dendritic spine density (104 spines/100 um or 99 spines/100 um in wild-type hippocampal or cortical (layer 1) neurons compared to 89 spines/100 um or 79.8 spines/100 um in mutant hippocampal or cortical neurons)

abnormal neuron physiology ( J:117303 )

• cultured E18 hippocampal neurons show 60.1% less neurite outgrowth (sum of axonal and dendrite length) than wild-type neurons after 5 days

• leading edge of growth cone rarely extended to tips of filopodia

• lamellipodia extend at rate of 6.85 um/minute compared to 5.84 um/minute observed in wild-type cultured neurons, but retraction velocity is similar for both genotypes

abnormal CNS synaptic transmission ( J:117303 )

abnormal excitatory postsynaptic potential ( J:117303 )

• hippocampal slices from mutants display a 29% reduction in maximal fEPSPs

abnormal NMDA-mediated synaptic currents ( J:117303 )

• NMDAR to AMPA receptor fEPSP ratio is significantly higher in null neurons

enhanced long-term potentiation ( J:117303 )

• early phase of LTP is increased relative to wild-type (seen in responses from 0-60 minutes)

• L-LTP induced by rapid theta bursts results in modest increase in potentiation at 150-180 minutes

reduced long-term depression ( J:117303 )

• LTD is reduced relative to wild-type (seen in responses from 0-60 minutes)