Phenotypes associated with this allele

• Allele Symbol: Wasl^tm1Sbs
• Allele Name: targeted mutation 1, Scott B Snapper
• Allele ID: MGI:2651836
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wasl^tm1Sbs/Wasl^tm1Sbs	either: (involves: 129P2/OlaHsd * 129S/SvEv) or (involves: 129P2/OlaHsd * C57BL/6)	MGI:2651837

Genotype
MGI:2651837

hm1

• Allelic Composition: Wasl^tm1Sbs/Wasl^tm1Sbs
• Genetic Background: either: (involves: 129P2/OlaHsd * 129S/SvEv) or (involves: 129P2/OlaHsd * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:72053 )

• although homozygotes develop normally through gastrulation, they fail to survive beyond E11

embryo

delayed embryo turning ( J:72053 )

• at E8.5, some mutant embryos appear unturned

embryonic growth retardation ( J:72053 )

• as early as E8.5, mutant embryos appear developmentally retarded relative to wild-type embryos

decreased embryo size ( J:72053 )

• at E8.0-E11.0, mutant embryos are significantly smaller than wild-type embryos

wavy neural tube ( J:72053 )

• as early as E8.5, mutant embryos show prominent undulation of the neural tube

• however, the neural tube is closed

cardiovascular system

dilated heart ( J:72053 )

• at E8.0-E11.0, mutant embryos display a dilated heart filled with red blood cells

absent heartbeat ( J:72053 )

• mutant embryos exhibit absence of a heartbeat, even at E11.0

• however, early mutant embryos can generate spontaneously contracting cardiomyocytes (not shown)

nervous system

wavy neural tube ( J:72053 )

• as early as E8.5, mutant embryos show prominent undulation of the neural tube

• however, the neural tube is closed

growth/size/body

embryonic growth retardation ( J:72053 )

• as early as E8.5, mutant embryos appear developmentally retarded relative to wild-type embryos

decreased embryo size ( J:72053 )

• at E8.0-E11.0, mutant embryos are significantly smaller than wild-type embryos

cellular

abnormal cell chemotaxis ( J:72053 )

• although mutant fibroblast-like cells (FLCs) derived from ES cells can form prominent lamellipodia and filopodia in response to stimulation with serum or platelet-derived growth factor (PDGF), they show distinct defects in the actin-based motility of certain pathogens

• whereas the actin-based movement of the intracellular pathogen Listeria is largely normal, the motility of Shigella and vaccinia virus is severely blunted in mutant FLCs

decreased fibroblast proliferation ( J:72053 )

• unlike wild-type MEFs, fibroblasts isolated from E9.5 mutant embryos are initially viable but fail to expand

• in contrast, mutant FLCs derived from either ES cells or SV40-transformed embryos display normal growth rates relative to wild-type FLCs