All Phenotypes Ptger2<tm1Sna> MGI Mouse

• homozygotes display abortive cumulus expansion, indicating defects in cumulus cell function, and consequently oocyte maturation

mortality/aging ( J:57482 )

N	• homozygotes develop normally, gain weight at a normal rate, and have lifespans of >1 year

homeostasis/metabolism phenotype ( J:70534 )

N	• homozygotes exhibit a normal febrile response to i.c.v. administration of 1 nmol PGE2 and to i.v. injection of IL-1-beta relative to wild-type mice

abnormal oocyte morphology ( J:57482 )

• homozygotes display abortive cumulus expansion, indicating defects in cumulus cell function, and consequently oocyte maturation

decreased corpora lutea number ( J:57482 )

• following superovulation with exogenous gonadotropins, pregnant females show a slight reduction in the number of corpora lutea

abnormal cumulus expansion ( J:70534 )

• in vivo, ovulated cumulus-oocyte complexes recovered from mutant oviducts before fertilization show abnormal aggregation, and cumulus cells appear less expanded

absent cumulus expansion ( J:57482 )

• in vitro, cumuli oophori isolated from mutant mice fail to exhibit PGE2- or butaprost-elicicted cumulus expansion

• in contrast, normal expansion is observed upon treatment with FSH or dibutyryl cAMP

decreased ovulation rate ( J:57482 )

• female homozygotes display a slight reduction in ovulation in the absence of a pituitary gonadotropin deficiency

• in contrast to reduced ovulation, both implantation and decidualization appear unaffected

reduced female fertility ( J:57482 )

decreased litter size ( J:57482 )

• female homozygotes produce small litter sizes irrespective of the genotype of mating males; very few pups (~1.0 per pregnancy, on average) are born alive and grow normally

• a similar reduction in litter size is noted with live fetuses studied in utero on E19

impaired fertilization ( J:57482 )

• female homozygotes exhibit severely impaired fertilization, with only 8.5% of recovered eggs being fertilized versus 55% in wild-type females

• however, no difference in the fertilization rate is detected in in vitro studies using cumulus-free oocytes