All Phenotypes Cd59b<tm1Jha> MGI Mouse

early reproductive senescence ( J:82330 )

• male homozygotes were fertile until ~5 months of age; thereafter, mutant males began to progressively lose fertility and eventually became infertile

hemolytic anemia ( J:82330 )

• homozygotes displayed hemolytic anemia with spontaneous platelet activation

• mutant erythrocytes were more sensitive to complement-induced lysis relative to wild-type

erythroblastosis ( J:82330 )

decreased erythrocyte cell number ( J:82330 )

decreased hematocrit ( J:82330 )

decreased hemoglobin content ( J:82330 )

anisopoikilocytosis ( J:82330 )

echinocytosis ( J:82330 )

schistocytosis ( J:82330 )

reticulocytosis ( J:82330 )

abnormal platelet physiology ( J:82330 )

• FACS analysis revealed an 8-fold increase in the shedding of platelet microparticles, indicating platelet activation; electron microscopy confirmed the formation of stubby and elongated filopodia and platelet aggregation

homeostasis/metabolism phenotype ( J:108100 )

N	• at 6 months of age, male homozygotes showed no significant differences in serum FSH, LH, and testosterone levels relative to wild-type controls

abnormal platelet physiology ( J:82330 )

• FACS analysis revealed an 8-fold increase in the shedding of platelet microparticles, indicating platelet activation; electron microscopy confirmed the formation of stubby and elongated filopodia and platelet aggregation

hemoglobinemia ( J:82330 )

hemoglobinuria ( J:82330 )

hemosiderinuria ( J:82330 )

hemoglobinuria ( J:82330 )

hemosiderinuria ( J:82330 )

reproductive system phenotype ( J:82330 , J:108100 )

N

• male homozygotes exhibited normal sexual behavior toward females and generated copulation plugs independently of their age (J:82330)

• at 3 months of age, mutant epididymal sperm exhibited normal capacitation-dependent tyrosine phosphorylation and ionophore-induced acrosome reaction relative to wild-type controls (J:108100)

necrospermia ( J:108100 )

• starting at 3 months of age, male homozygotes displayed a progressive decline in the % of viable sperm

• by 6 months of age, the % of motile and viable sperm was significantly decreased, whereas the % of abnormal sperm heads was significantly increased

abnormal male germ cell morphology ( J:82330 )

• histology demonstrated loss of spermatozoid's head and multinucleated cells with increased cytoplasm relative to wild-type

oligozoospermia ( J:82330 , J:108100 )

• cresyl violet-stained sperm smears revealed a significant reduction in the number of spermatozoids in infertile males (J:82330)

• at 3 months of age, male homozygotes showed a 2.5-fold reduction in the number of motile and live sperm obtained by the swim-out method from the epididymis relative to wild-type controls (J:108100)

• by 6 months of age, male homozygotes displayed a significant decrease in the total number of sperm relative to wild-type controls (J:108100)

abnormal sperm head morphology ( J:108100 )

• starting at 3 months of age, male homozygotes displayed a significantly higher % of abnormal sperm heads than wild-type controls

absent sperm head ( J:108100 )

• detached sperm heads were frequently observed at 6 months of age

multinucleated giant male germ cells ( J:82330 , J:108100 )


	(J:82330)

increased male germ cell apoptosis ( J:108100 )

• at 6 months of age, male homozygotes displayed a significantly higher number of apoptotic (TUNEL-positive) germ cells in seminiferous tubules than age-matched wild-type controls

asthenozoospermia ( J:82330 )

• almost all sperm cells from infertile mutant males were immobile

• in some instances, one or two sperm cells exhibited contraction-like movements without displacement

abnormal seminiferous tubule morphology ( J:108100 )

• at 6 and 10 months of age, 33% of male homozygotes displayed abnormal multinucleated cells and significantly more apoptotic cells within the walls of seminiferous tubules

• however, no signs of testicular atrophy, inflammation or of increased complement deposition were observed