Analysis Tools|
Allele Symbol Allele Name Allele ID |
Paxip1tm1Gdr targeted mutation 1, Gregory R Dressler MGI:2653321 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• embryonic lethality occurs before E9.5
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• embryos are disorganized and degenerating at E9.5
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• embryos are disorganized and degenerating at E9.5
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• homozygotes are embryonic lethal by E9.5
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• at E9.5, mutant embryos are significantly smaller than wild-type embryos
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• at E9.5, mutant embryos show little vascularization of the yolk sac
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• at E7.5, TUNEL staining revealed significant cell death within the embryonic ectodermal layer, but not within the extraembryonic and ectoplacental tissues
• remarkably, many mutant nuclei stain positive in the TUNEL assay even before nuclear condensation occurs, whereas wild-type TUNEL-positive nuclei are almost always pyknotic
• at E8.5, apoptosis is more widespread than at E7.5, and is particularly high in the embryo proper
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• blastocyst outgrowths from E3.5 mutant embryos exhibit inhibition of inner cell mass proliferation upon explant culture
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• at E9.5, mutant embryos exhibit a poorly defined body axis
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• at E9.5, mutant embryos appear to be developmentally arrested and severely disorganized relative to wild-type embryos
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• at E9.5, mutant embryos are significantly smaller than wild-type embryos
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• at E7.5, the egg cylinder is about half as long as that of a wild-type embryos
• in contrast, the ectoplacental cone is well developed
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• at E9.5, only occasional somites are present, often unilaterally
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• at E9.5, mutant embryos resemble disorganized E8.5 embryos
• however, the head folds and neural tube are present in most cases
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• at E9.5, the allantois has not yet reached the placenta
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• at E7.5, mutant embryos lack a clearly defined amnion
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• at E9.5, mutant embryos show little vascularization of the yolk sac
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• at E9.5, mutant embryos display no signs of heart development
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• the number of mitotic cells with condensed chromatin is reduced at E7.5 and severely limited by E8.5
• at E7.5, centrosomes are undetected, the microtubules are poorly organized, and chromatin condensation appears abnormal with suppressed levels of phospho-histone H3 and little evidence of segregation
• by E8.5, only a few cells are shown to progress through mitosis
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• mutant trophoblast cells exhibit increased sensitivity to 2 Gy of ionizing radiation, with a 50% reduction in the number of viable trophoblast cells after 7 days in culture
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• at E7.5, TUNEL staining revealed significant cell death within the embryonic ectodermal layer, but not within the extraembryonic and ectoplacental tissues
• remarkably, many mutant nuclei stain positive in the TUNEL assay even before nuclear condensation occurs, whereas wild-type TUNEL-positive nuclei are almost always pyknotic
• at E8.5, apoptosis is more widespread than at E7.5, and is particularly high in the embryo proper
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• at E8.5, BrdU-positive cells are found only in regions that show less TUNEL staining or in the amniotic membrane; in contrast, BrdU-positive cells are abundant in wild-type embryos, representing ~52% of the total nuclei
• neither embryonic fibroblast nor embryonic stem cells isolated from mutant embryos proliferate in culture, suggesting a severe defect in cell proliferation
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• blastocyst outgrowths from E3.5 mutant embryos exhibit inhibition of inner cell mass proliferation upon explant culture
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• DNA damage appears to occur prior to nuclear condensation at E7.5, suggesting a defect in DNA repair
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• although DNA replication is relatively normal at E7.5 when cell death is first detected, no DNA replication occurs in most of the embryo proper at E8.5, as cell death becomes widespread
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• DNA damage appears to occur prior to nuclear condensation at E7.5, suggesting a defect in DNA repair
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• although DNA replication is relatively normal at E7.5 when cell death is first detected, no DNA replication occurs in most of the embryo proper at E8.5, as cell death becomes widespread
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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