Phenotypes associated with this allele

• Allele Symbol: Plcb2^tm1Dwu
• Allele Name: targeted mutation 1, Dianqing Wu
• Allele ID: MGI:2653348
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Plcb2^tm1Dwu/Plcb2^tm1Dwu	B6.129S1-Plcb2^tm1Dwu/J	MGI:5775112
hm2	Plcb2^tm1Dwu/Plcb2^tm1Dwu	involves: 129	MGI:2653350
hm3	Plcb2^tm1Dwu/Plcb2^tm1Dwu	involves: 129/Sv * SWR	MGI:2653357
cx4	Plcb2^tm1Dwu/Plcb2^tm1Dwu Plcb3^tm1Dwu/Plcb3^tm1Dwu	involves: 129S1/Sv	MGI:4358152
cx5	Plcb2^tm1Dwu/Plcb2^tm1Dwu Plcb3^tm1Dwu/Plcb3^tm1Dwu	involves: 129S1/Sv * CD-1	MGI:4358173

Genotype
MGI:5775112

hm1

• Allelic Composition: Plcb2^tm1Dwu/Plcb2^tm1Dwu
• Genetic Background: B6.129S1-Plcb2^tm1Dwu/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal sensory capabilities/reflexes/nociception ( J:223366 )

♀ • during estrus, female mice fail to respond behaviorally with attraction to rMUPs unlike wild-type mice

♀ • however, mice fully detected and avoid FELD4

nervous system

abnormal vomeronasal sensory neuron physiology ( J:223366 )

♀ • vomeronasal sensory neurons (VSNs) from estrous females fail to response to recombinant major urinary proteins (rMUPs) stimulation unlike wild-type neurons

♀ • however, response to non-rMUP stimuli is robust

Genotype
MGI:2653350

hm2

• Allelic Composition: Plcb2^tm1Dwu/Plcb2^tm1Dwu
• Genetic Background: involves: 129

immune system

abnormal leukocyte physiology ( J:41948 )

• chemotaxic responses of leukocytes are enhanced

decreased susceptibility to infection ( J:41948 )

• enhanced resistance to both bacterial and viral infection

decreased susceptibility to bacterial infection ( J:41948 )

decreased susceptibility to viral infection ( J:41948 )

hematopoietic system

abnormal leukocyte physiology ( J:41948 )

• chemotaxic responses of leukocytes are enhanced

Genotype
MGI:2653357

hm3

• Allelic Composition: Plcb2^tm1Dwu/Plcb2^tm1Dwu
• Genetic Background: involves: 129/Sv * SWR

taste/olfaction

abnormal gustatory system physiology ( J:82314 )

• nerve responses to sweet, amino acid, and bitter stimuli were abolished

• normal nerve responses to salty or sour stimuli

abnormal taste sensitivity ( J:82314 )

• preference for sweet, amino acid or bitter vs. water abolished

• preference for salty or sour vs. water similar to controls

abnormal bitter taste sensitivity ( J:82314 )

abnormal sweet taste sensitivity ( J:82314 )

Genotype
MGI:4358152

cx4

• Allelic Composition: Plcb2^tm1Dwu/Plcb2^tm1Dwu; Plcb3^tm1Dwu/Plcb3^tm1Dwu
• Genetic Background: involves: 129S1/Sv

immune system

abnormal neutrophil physiology ( J:60348 )

• neutrophils exhibit increased chemotactic activities in response to CC chemokine MIP-1alpha (Ccl3) compared with similarly treated wild-type cells

• in E. coli-induced peritonitis, neutrophil infiltration into the peritoneal cavity is enhanced compared to in similarly treated wild-type mice

increased immunoglobulin level ( J:60348 )

• mice treated with NP-Ficoll produce more antibodies containing lambda light chain compared with similarly treated wild-type mice

integument

spontaneous skin ulceration ( J:60348 )

hematopoietic system

abnormal neutrophil physiology ( J:60348 )

• neutrophils exhibit increased chemotactic activities in response to CC chemokine MIP-1alpha (Ccl3) compared with similarly treated wild-type cells

• in E. coli-induced peritonitis, neutrophil infiltration into the peritoneal cavity is enhanced compared to in similarly treated wild-type mice

increased immunoglobulin level ( J:60348 )

• mice treated with NP-Ficoll produce more antibodies containing lambda light chain compared with similarly treated wild-type mice

Genotype
MGI:4358173

cx5

• Allelic Composition: Plcb2^tm1Dwu/Plcb2^tm1Dwu; Plcb3^tm1Dwu/Plcb3^tm1Dwu
• Genetic Background: involves: 129S1/Sv * CD-1

homeostasis/metabolism

increased platelet ATP level ( J:60348 )

• collagen-mediated platelet secretion is mildly enhanced compared to in similarly treated wild-type cells

decreased platelet calcium level ( J:60348 )

• thrombin- or ADP-stimulated calcium mobilization in platelets is decreased 58% and 43%, respectively, compared to in similarly treated wild-type cells

abnormal platelet physiology ( J:60348 )

• spreading of platelets on fibrinogen is impaired compared with wild-type cell

decreased platelet aggregation ( J:60348 )

• at low doses of ADP, thrombin, or collagen

decreased susceptibility to induced thrombosis ( J:60348 )

• following ferric chloride-induced arterial injury, mice fail to form arterial occlusions unlike in similarly treated wild-type mice

hematopoietic system

increased platelet ATP level ( J:60348 )

• collagen-mediated platelet secretion is mildly enhanced compared to in similarly treated wild-type cells

decreased platelet calcium level ( J:60348 )

• thrombin- or ADP-stimulated calcium mobilization in platelets is decreased 58% and 43%, respectively, compared to in similarly treated wild-type cells

abnormal platelet physiology ( J:60348 )

• spreading of platelets on fibrinogen is impaired compared with wild-type cell

decreased platelet aggregation ( J:60348 )

• at low doses of ADP, thrombin, or collagen

cellular

increased platelet ATP level ( J:60348 )

• collagen-mediated platelet secretion is mildly enhanced compared to in similarly treated wild-type cells