Phenotypes associated with this allele

• Allele Symbol: Rag2^tm1Cgn
• Allele Name: targeted mutation 1, University of Cologne
• Allele ID: MGI:2654906
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rag2^tm1Cgn/Rag2^tm1Cgn	C57BL/6-Rag2^tm1Cgn	MGI:2654914
cn2	Rag2^tm1Cgn/Rag2^tm1Cgn Xrcc4^tm1Fwa/Xrcc4^tm2Fwa Tg(Cr2-cre)3Cgn/?	involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6	MGI:4352688
cn3	Rag2^tm1Cgn/Rag2^tm1.1Cgn Tg(Mx1-cre)1Cgn/0	involves: C57BL/6 * CBA	MGI:2654913
cx4	Mog^tm1Dpd/Mog^tm1Dpd Rag2^tm1Cgn/Rag2^tm1Cgn Tg(Tcra2D2,Tcrb2D2)1Kuch/0	involves: 129S2/SvPas * C57BL/6	MGI:4461062

Genotype
MGI:2654914

hm1

• Allelic Composition: Rag2^tm1Cgn/Rag2^tm1Cgn
• Genetic Background: C57BL/6-Rag2^tm1Cgn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:72214 )

• B cell lymphopoiesis in the bone marrow is normal

• the number of B cells found in circulation is comparable to that of wild-type mice

Genotype
MGI:4352688

cn2

• Allelic Composition: Rag2^tm1Cgn/Rag2^tm1Cgn; Xrcc4^tm1Fwa/Xrcc4^tm2Fwa; Tg(Cr2-cre)3Cgn/?
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6

cellular

spontaneous chromosome breakage ( J:150339 )

• genomic stability is disrupted in activated B cells with chromosome breakage occurring in close to half of cells

• B cells undergoing class switch recombination have even greater increased risk of chromosome breakage due to aberrations at the Igh locus

• there is decreased breakage at Igl locus compared to conditional knockouts on a Rag-sufficient background

hematopoietic system

abnormal class switch recombination ( J:150339 )

• class switch recombination often leads to chromosome breakage at the Igh locus

immune system

abnormal class switch recombination ( J:150339 )

• class switch recombination often leads to chromosome breakage at the Igh locus

Genotype
MGI:2654913

cn3

• Allelic Composition: Rag2^tm1Cgn/Rag2^tm1.1Cgn; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6 * CBA

immune system

absent immature B cells ( J:72214 )

• the IgM⁺HSA^high immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old

arrested B cell differentiation ( J:72214 )

• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old

arrested T cell differentiation ( J:72214 )

• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old

decreased mature B cell number ( J:72214 )

• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days

• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction

• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year

• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction

• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression

• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal

decreased follicular B cell number ( J:72214 )

• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days

decreased marginal zone B cell number ( J:72214 )

• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth

• the number of marginal zone B cells increases slightly with age but remains well below normal levels

• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age

increased plasma cell number ( J:72214 )

• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls

absent pre-B cells ( J:72214 )

• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old

abnormal B cell activation ( J:72214 )

• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth

• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age

• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age

hematopoietic system

absent immature B cells ( J:72214 )

• the IgM⁺HSA^high immature B cell compartment in the spleen is absent two weeks after induction of Cre expression in mice 8-10 weeks old

arrested B cell differentiation ( J:72214 )

• B cell development is arrested at the pro-B cell stage two weeks after induction of Cre expression in mice 8-10 weeks old

arrested T cell differentiation ( J:72214 )

• T cell development is arrested at the pro-T cell stage two weeks after induction of Cre expression in mice 8-10 weeks old

decreased mature B cell number ( J:72214 )

• the number of mature B cells in the bone marrow drop by half within two weeks of induction of Cre expression in mice 8-10 weeks old and then decline slowly over the next year with a half-life of 156 days

• the number of mature B cells in the spleen is reduced by 67% two weeks after Cre induction

• mature B cell numbers are reduced to 32% in the spleen 17 weeks after Cre induction and remain at this level for at least one year

• mature B cell numbers in lymph nodes drop to 58% of normal 10 weeks after Cre induction, and continue to drop to 11% of normal 17 weeks after Cre induction

• mature B cell numbers remain at 11% of normal between 17 and 54 weeks after induction of Cre expression

• when Cre is induced in neonates, mature B cells numbers are 10- to 30- fold below normal

decreased follicular B cell number ( J:72214 )

• the number of follicular B cells in the spleen decline slowly after induction of Cre expression in mice 8-10 weeks old with a half-life of 134 days

decreased marginal zone B cell number ( J:72214 )

• marginal zone B cells are decreased in number by 75% when Cre-induction occurs at birth

• the number of marginal zone B cells increases slightly with age but remains well below normal levels

• marginal zone B cell numbers are normal when Cre-induction occurs in mice 8 to 10 weeks of age

increased plasma cell number ( J:72214 )

• the total number of IgM secreting plasma cells found in the spleen is 3- to 4- fold larger in mice where Cre induction is induced at birth than in controls

absent pre-B cells ( J:72214 )

• pre-B cells are absent in the bone marrow of mice two weeks after induction of Cre expression in mice 8-10 weeks old

abnormal B cell activation ( J:72214 )

• peripheral B cells have an activated phenotype in mice that have Cre expression induced at birth

• the activated phenotype include larger cell size and increased expression of CD25, CD69, CD86 and MHC-II when analyzed at 8 weeks of age

• B cells do not have this activated phenotype when Cre is induced in mice 8-10 weeks of age

Genotype
MGI:4461062

cx4

• Allelic Composition: Mog^tm1Dpd/Mog^tm1Dpd; Rag2^tm1Cgn/Rag2^tm1Cgn; Tg(Tcra2D2,Tcrb2D2)1Kuch/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6

immune system

increased susceptibility to experimental autoimmune encephalomyelitis ( J:151335 )

• spontaneous EAE develops in two out of six mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
multiple sclerosis		DOID:2377	OMIM:612594 OMIM:612595 OMIM:612596	J:151335