All Phenotypes Pafah1b3<tm1Arai> MGI Mouse

abnormal male germ cell morphology ( J:82792 )

• at 5 weeks of age, some double mutant germ cells appear to be detached from the Sertoli cells

decreased male germ cell number ( J:82792 )

• at 5 weeks of age, double mutant males show a significant reduction of spermatogenic cells, especially after the pachytene spermatocyte stage; round spermatids are significantly reduced in number, while elongated spermatids are rare

• at later ages, early germ cell stages are more severely affected, leading to pronounced depletion of spermatocytes and spermatogonia

azoospermia ( J:82792 )

• no spermatozoa are detected in the epididymis at 5 weeks of age

increased male germ cell apoptosis ( J:82792 )

• at 5 weeks of age, double mutant testes display increased apoptosis of spermatogenic cells, as shown by TUNEL staining

• the majority of cells undergoing apoptosis are spermatocytes

small seminiferous tubules ( J:82792 )

• at 5 weeks of age, double mutant males show a ~50% reduction in seminiferous tubule diameter relative to wild-type males

small testis ( J:82792 )

decreased testis weight ( J:82792 )

• at 5 weeks of age, double mutant testes weigh ~65% less than those of wild-type mice

abnormal spermatogenesis ( J:82792 )

• double mutant males exhibit defects in several aspects of spermatogenesis, including severe degeneration of primary spermatocytes as well as degeneration of meiotically dividing spermatocytes and elongating spermatids

abnormal spermatid morphology ( J:82792 )

• at 5 weeks of age, the few remaining spermatids exhibit deformed nuclei

male infertility ( J:82792 )

• double mutant males are infertile

• in contrast, double mutant females are fertile