Phenotypes associated with this allele

• Allele Symbol: Gja8^tm1(Gja3)Tww
• Allele Name: targeted mutation 1, Thomas W White
• Allele ID: MGI:2656266
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gja8^tm1(Gja3)Tww/Gja8^tm1(Gja3)Tww	involves: 129S/SvEv	MGI:2656271
cx2	Crygb^S11R/Crygb^S11R Gja8^tm1(Gja3)Tww/Gja8^tm1(Gja3)Tww	involves: A/J * C57BL/6J	MGI:6099062

Genotype
MGI:2656271

hm1

• Allelic Composition: Gja8^tm1(Gja3)Tww/Gja8^tm1(Gja3)Tww
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

vision/eye

small lens ( J:73875 )

• at P7, mutant lenses are proportionately smaller but show normal cellular histology

• by P14, the mutant lens mass is reduced by 34% relative to the wild-type

• however, no significant differences in lens mass are noted at P0, and eye and lens mass ratios remain constant after P14

• in addition, adult mutant lenses lack cataracts, indicating normal attainment and maintenance of lens transparency

abnormal eye development ( J:73875 )

• in mutant mice, the postnatal ocular growth rate lags behind that of wild-type littermates

abnormal lens development ( J:73875 )

• in mutant mice, the postnatal lens growth rate lags behind that of wild-type littermates

• however, mutant lenses synthesize markers of lens differentiation at appropriate levels and exhibit normal crystallin solubility

microphthalmia ( J:73875 )

• at P7, mutant eyes are proportionately smaller but show normal anatomy

• by P14, the mutant eye mass is reduced by 25% relative to the wild-type

• however, no significant differences in eye mass are noted at P0, and eye and lens mass ratios remain constant after P14

Genotype
MGI:6099062

cx2

• Allelic Composition: Crygb^S11R/Crygb^S11R; Gja8^tm1(Gja3)Tww/Gja8^tm1(Gja3)Tww
• Genetic Background: involves: A/J * C57BL/6J

vision/eye

vision/eye phenotype ( J:165131 )

N • the enlarged extracellular spaces and disrupted fiber-to-fiber contacts in the fiber cells of the lens core of crystallin gamma b mutants is rescued in these double homozygotes

abnormal lens morphology ( J:165131 )

• while the alpha 3 connexin knockin rescues the dense nuclear cataract otherwise caused by the crystallin gamma b mutation, a very mild cortical opacity with a ring-like defect is still found in these double homozygotes and the lenses have much lower light scattering at 21 days of age compared with un-rescued crystallin gamma b mutants, but is still 6% higher than wild-type controls