Phenotypes associated with this allele

• Allele Symbol: Amot^tm1Bhr
• Allele Name: targeted mutation 1, Richard R Behringer
• Allele ID: MGI:2656627
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Amot^tm1Bhr/Amot^tm1Bhr	B6.129S7-Amot^tm1Bhr	MGI:3832448
hm2	Amot^tm1Bhr/Amot^tm1Bhr	involves: 129S7/SvEvBrd * C57BL/6	MGI:2656632
ot3	Amot^tm1Bhr/Y	B6.129S7-Amot^tm1Bhr	MGI:3832462
ot4	Amot^tm1Bhr/Y	involves: 129S7/SvEvBrd * C57BL/6	MGI:2656633

Genotype
MGI:3832448

hm1

• Allelic Composition: Amot^tm1Bhr/Amot^tm1Bhr
• Genetic Background: B6.129S7-Amot^tm1Bhr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:123910 )

♀ • 75% of mutant mice die between E11 and E11.5

growth/size/body

decreased embryo size ( J:123910 )

♀ • embryos are 85% the size of control littermates at E10.5

embryo

abnormal vitelline vasculature morphology ( J:123910 )

♀ • E10.5 yolk sacs exhibit abnormal vascular patterning, containing vessels with increased diameter and some vessels that are anastomosed to form lagoon-like structures

decreased embryo size ( J:123910 )

♀ • embryos are 85% the size of control littermates at E10.5

small placenta ( J:123910 )

♀ • placentas are 75% the size of control littermate placentas at E10.5, however the establishment of the placental layers and function are normal

cardiovascular system

abnormal capillary morphology ( J:123910 )

♀ • insufficient capillary formation in the somitic region

abnormal brain vasculature morphology ( J:123910 )

♀ • mutants exhibit a less structured network of blood vessels in the brain that form lagoon-like structures with an accumulation of erythrocytes

♀ • branching is not affected, however vessel diameter in the brain is twice the size of wild-type

abnormal angiogenesis ( J:123910 )

♀ • severe vascular insufficiency in the intersomitic region; the interconnecting paraneural blood vessels are missing and the remaining vessels are dilated and shorter

abnormal vitelline vasculature morphology ( J:123910 )

♀ • E10.5 yolk sacs exhibit abnormal vascular patterning, containing vessels with increased diameter and some vessels that are anastomosed to form lagoon-like structures

dilated vasculature ( J:123910 )

♀ • dilated vessels in the brain and yolk sac

nervous system

abnormal brain vasculature morphology ( J:123910 )

♀ • mutants exhibit a less structured network of blood vessels in the brain that form lagoon-like structures with an accumulation of erythrocytes

♀ • branching is not affected, however vessel diameter in the brain is twice the size of wild-type

Genotype
MGI:2656632

hm2

• Allelic Composition: Amot^tm1Bhr/Amot^tm1Bhr
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:82844 )

♀ • 50% of mutant embryos die around E7.5

growth/size/body

embryonic growth retardation ( J:82844 )

♀ • embryos are developmentally delayed by about 6 hours at E7.5 and sometimes up to 12 hours or more at E8

embryo

abnormal anterior visceral endoderm cell migration ( J:82844 )

♀ • visceral endoderm cell migration at the anterior embryonic/extra-embryonic junction is abnormal; although distal-to-proximal migration is observed, the lateral migration of cells is greatly reduced

abnormal developmental patterning ( J:82844 )

♀ • ~ 90% of embryos at E7.5 have excessive folds at the cranial limiting furrow region

♀ • the cranial limiting furrow region contains more than two cell layers and is disorganized

embryonic growth retardation ( J:82844 )

♀ • embryos are developmentally delayed by about 6 hours at E7.5 and sometimes up to 12 hours or more at E8

abnormal visceral endoderm morphology ( J:82844 )

♀ • visceral endoderm accumulates at the cranial limiting furrow of E7.5 mutants

cardiovascular system

hemorrhage ( J:82844 )

♀ • 62% of embryos are hemorrhagic at E7.5

cellular

abnormal anterior visceral endoderm cell migration ( J:82844 )

♀ • visceral endoderm cell migration at the anterior embryonic/extra-embryonic junction is abnormal; although distal-to-proximal migration is observed, the lateral migration of cells is greatly reduced

Genotype
MGI:3832462

ot3

• Allelic Composition: Amot^tm1Bhr/Y
• Genetic Background: B6.129S7-Amot^tm1Bhr

mortality/aging

embryonic lethality during organogenesis, incomplete penetrance ( J:123910 )

♂ • 75% of mutant mice die between E11 and E11.5

growth/size/body

decreased embryo size ( J:123910 )

♂ • embryos are 85% the size of control littermates at E10.5

embryo

abnormal vitelline vasculature morphology ( J:123910 )

♂ • E10.5 yolk sacs exhibit abnormal vascular patterning, containing vessels with increased diameter and some vessels that are anastomosed to form lagoon-like structures

decreased embryo size ( J:123910 )

♂ • embryos are 85% the size of control littermates at E10.5

small placenta ( J:123910 )

♂ • placentas are 75% the size of control littermate placentas at E10.5, however the establishment of the placental layers and function are normal

cardiovascular system

abnormal capillary morphology ( J:123910 )

♂ • insufficient capillary formation in the somitic region

abnormal brain vasculature morphology ( J:123910 )

♂ • mutants exhibit a less structured network of blood vessels in the brain that form lagoon-like structures with an accumulation of erythrocytes

♂ • branching is not affected however vessel diameter in the brain is twice the size of wild-type

abnormal angiogenesis ( J:123910 )

♂ • severe vascular insufficiency in the intersomitic region; the interconnecting paraneural blood vessels are missing and the remaining vessels are dilated and shorter

♂ • VEGF-induced tubulogenesis is impaired in embryoid bodies

abnormal vitelline vasculature morphology ( J:123910 )

♂ • E10.5 yolk sacs exhibit abnormal vascular patterning, containing vessels with increased diameter and some vessels that are anastomosed to form lagoon-like structures

dilated vasculature ( J:123910 )

♂ • dilated vessels in the brain and yolk sac

nervous system

abnormal brain vasculature morphology ( J:123910 )

♂ • mutants exhibit a less structured network of blood vessels in the brain that form lagoon-like structures with an accumulation of erythrocytes

♂ • branching is not affected however vessel diameter in the brain is twice the size of wild-type

Genotype
MGI:2656633

ot4

• Allelic Composition: Amot^tm1Bhr/Y
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:82844 )

♂ • 50% of embryos die around E7.5

growth/size/body

embryonic growth retardation ( J:82844 )

♂ • embryos are developmentally delayed by about 6 hours at E7.5 and sometimes up to 12 hours or more at E8

embryo

abnormal anterior visceral endoderm cell migration ( J:82844 )

♂ • visceral endoderm cell migration at the anterior embryonic/extra-embryonic junction is abnormal; although distal-to-proximal migration is observed, the lateral migration of cells is greatly reduced

abnormal developmental patterning ( J:82844 )

♂ • ~ 90% of embryos at E7.5 have excessive folds at the cranial limiting furrow region

♂ • the cranial limiting furrow region contains more than two cell layers and is disorganized

embryonic growth retardation ( J:82844 )

♂ • embryos are developmentally delayed by about 6 hours at E7.5 and sometimes up to 12 hours or more at E8

abnormal visceral endoderm morphology ( J:82844 )

♂ • visceral endoderm accumulates at the cranial limiting furrow of E7.5 mutants

cardiovascular system

hemorrhage ( J:82844 )

♂ • 62% of embryos are hemorrhagic at E7.5

cellular

abnormal anterior visceral endoderm cell migration ( J:82844 )

♂ • visceral endoderm cell migration at the anterior embryonic/extra-embryonic junction is abnormal; although distal-to-proximal migration is observed, the lateral migration of cells is greatly reduced