Phenotypes associated with this allele

• Allele Symbol: Cd74^tm3Liz
• Allele Name: targeted mutation 3, Elizabeth K Bikoff
• Allele ID: MGI:2656764
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd74^tm3Liz/Cd74^tm3Liz	involves: 129S/SvEv * C57BL/6	MGI:3688850

Genotype
MGI:3688850

hm1

• Allelic Composition: Cd74^tm3Liz/Cd74^tm3Liz
• Genetic Background: involves: 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:82880 )

N • comparable numbers of CD4+ T cells to wild-type are found in thymus and periphery

N • proliferative responses of mutant CD4+ T cells to antigen stimulation are similar to wild-type

abnormal antigen presentation via MHC class II ( J:82880 )

• mutant cells exhibit similar antigen presentation activities to wild-type in presence of diverse CD4+ T cell clones

abnormal level of surface class II molecules ( J:82880 )

• compared to Cd74^tm1Liz spleen cells which have reduced levels of class II (I-A^b surface antigens), mutants have similar levels to wild-type or p31 expressing mutants

defective assembly of class II molecules ( J:82880 )

• mutants expressing only p41 invariant chain produce substantial amounts of assembled Aalpha^bAbeta^b dimers but in contrast to wild-type or p31 chain only expressing cells the Aalpha^b and Abeta^b chains show reduced mobility in SDS-PAGE gels

• spleen cells show both compact dimers and a small proportion of floppy conformers, whereas wild-type have only compact dimers and Cd74^tm1Liz cells show floppy conformers; cells expressing 2-fold greater p41 chain levels produce a small population of floppy conformers

• Aalpha^kAbeta^k assembly and maturation in mutants expressing the k haplotype is reconstituted and is similar to wild-type, in comparison to assembly in mutants expressing the b haplotype

decreased interleukin-2 secretion ( J:72243 )

• T cells from immunized mice secrete less Il-2 than wild-type cells, but show a similar proliferative response although one log greater concentration of p35-55 is needed to achieve the same level of interferon response

increased susceptibility to experimental autoimmune encephalomyelitis ( J:72243 )

• direct immunization with p35-55 results in development of experimental autoimmune encephalitis (EAE) similar to wild-type in incidence and severity