Phenotypes associated with this allele

• Allele Symbol: Ptgds^tm1Ohy
• Allele Name: targeted mutation 1, Osamu Hayaishi
• Allele ID: MGI:2657199
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptgds^tm1Ohy/Ptgds^tm1Ohy	B6.129P2-Ptgds^tm1Ohy	MGI:5517580
hm2	Ptgds^tm1Ohy/Ptgds^tm1Ohy	involves: 129P2/OlaHsd * C57BL/6	MGI:2657201
cx3	Apc^Min/Apc^+ Ptgds^tm1Ohy/Ptgds^tm1Ohy	involves: 129P2/OlaHsd * C57BL/6J	MGI:3700062

Genotype
MGI:5517580

hm1

• Allelic Composition: Ptgds^tm1Ohy/Ptgds^tm1Ohy
• Genetic Background: B6.129P2-Ptgds^tm1Ohy

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:197334 )

N • transferred bone marrow mast cells are able to restore passive cutaneous anaphylaxis response in Kit^W-sh homozygotes

decreased mast cell degranulation ( J:197334 )

• fewer degranulating ear mast cells in a model of IgE antigen-dependent passive cutaneous anaphylaxis despite normal numbers of mast cells

abnormal dermal mast cell morphology ( J:197334 )

• immature with less histamine

decreased mast cell histamine storage ( J:197334 )

• in dermal mast cells

decreased susceptibility to type I hypersensitivity reaction ( J:197334 )

• in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit decreased dye extravasation (a measure of edema) compared with wild-type mice

integument

abnormal dermal mast cell morphology ( J:197334 )

• immature with less histamine

homeostasis/metabolism

edema ( J:197334 )

• in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit decreased dye extravasation (a measure of edema) compared with wild-type mice

hematopoietic system

decreased mast cell degranulation ( J:197334 )

• fewer degranulating ear mast cells in a model of IgE antigen-dependent passive cutaneous anaphylaxis despite normal numbers of mast cells

abnormal dermal mast cell morphology ( J:197334 )

• immature with less histamine

decreased mast cell histamine storage ( J:197334 )

• in dermal mast cells

cellular

decreased mast cell degranulation ( J:197334 )

• fewer degranulating ear mast cells in a model of IgE antigen-dependent passive cutaneous anaphylaxis despite normal numbers of mast cells

Genotype
MGI:2657201

hm2

• Allelic Composition: Ptgds^tm1Ohy/Ptgds^tm1Ohy
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:52290 )

N • homozygotes display no significant differences in general behavior or in the growth curves of body weight up to 40 weeks of age relative to wild-type littermates

abnormal pain threshold ( J:52290 )

• unlike in wild-type mice, where i.t. administration of PGE2 (10 ng/mouse) induces mechanical allodynic responses at 5 min after injection which gradually decrease over the 50-min experimental period, i.t. injection of PGE2 (10 ng) fails to induce any allodynia at the spinal level in homozygous mutant mice

• also unlike in wild-type mice, where i.t. administration of bicuculline (a GABA type A receptor antagonist, 25 ng/mouse) induces mechanical allodynia over a 50-min experiment, the bicuculline-evoked allodynic response of homozygous mutant mice is significantly reduced after the first 15 min and disappears thereafter

• while i.t. injection of PGD2 alone fails to induce allodynia in either wild-type or homozygous mutant mice at doses up to 100 ng, simultaneous injection of a femtogram amount of PGD2 with PGE2 or bicuculline induces allodynia in mutant mice to the same extent as in wild-type mice

• in addition, i.t. injection of PGF2alpha (1 g) induces allodynia for 50 min to a similar extent in wild-type and homozygous mutant mice

integument

integument phenotype ( J:52290 )

N • homozygotes show no significant differences in thermal hyperalgesia evoked by PGD2 and PGE2 at the spinal level, as determined by measuring the response time to a hot plate (55C) at 10 and 30 min after intrathecal (i.t.) injection of exogenous PGD2 (5 ng/mouse) and PGE2 (10 ng/mouse), respectively

Genotype
MGI:3700062

cx3

• Allelic Composition: Apc^Min/Apc⁺; Ptgds^tm1Ohy/Ptgds^tm1Ohy
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

neoplasm

increased intestinal adenoma incidence ( J:118232 )

• mice with Ptgds deficiency do not show altered adenoma profile compared to Apc heterozygous Ptgds-wild-type mice

digestive/alimentary system

increased intestinal adenoma incidence ( J:118232 )

• mice with Ptgds deficiency do not show altered adenoma profile compared to Apc heterozygous Ptgds-wild-type mice