Phenotypes associated with this allele

• Allele Symbol: Nos3^tm1Weo
• Allele Name: targeted mutation 1, William E O'Brien
• Allele ID: MGI:2657240
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nos3^tm1Weo/Nos3^tm1Weo	B6.129S7-Nos3^tm1Weo	MGI:3041262
hm2	Nos3^tm1Weo/Nos3^tm1Weo	involves: 129	MGI:3041263
hm3	Nos3^tm1Weo/Nos3^tm1Weo	involves: 129S7/SvEvBrd * C57BL/6	MGI:2657241
cx4	Asl^tm1Brle/Asl^+ Nos3^tm1Weo/Nos3^+	involves: 129S7/SvEvBrd	MGI:5308985

Genotype
MGI:3041262

hm1

• Allelic Composition: Nos3^tm1Weo/Nos3^tm1Weo
• Genetic Background: B6.129S7-Nos3^tm1Weo

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal superovulation ( J:89703 )

♀ • in superovulated female mutants fewer large preovulatory follicles and corpora lutea are found in ovaries from homozygous mutant females compared to C57BL/6J wild-type mice

decreased superovulation rate ( J:73964 , J:89703 )

♀ • in superovulated female mutants on a C57BL/6J background but not on a 129/Sv background the number of oocytes released is significantly reduced compared to wild-type mice on the same background (J:73964)

♀ • in superovulated female mutants the number of oocytes released is significantly reduced compared to C57BL/6J wild-type mice (J:89703)

abnormal fertility/fecundity ( J:89703 )

♀ • the time between litters is longer for homozygous mutant females compared to C57BL/6J wild-type mice

early reproductive senescence ( J:89703 )

♀ • homozygous mutant females reached senescence earlier than C57BL/6J wild-type mice

delayed female fertility ( J:89703 )

♀ • the mean age of first pregnancy is significantly later for homozygous mutant females compared to C57BL/6J wild-type mice

impaired fertilization ( J:73964 )

• the number of in vitro fertilized embryos from female mutants on a C57BL/6J background that reach the two-cell stage and of these that go on to reach the blastocyst stage is significantly reduced compared to wild-type mice on the same background

mortality/aging

early reproductive senescence ( J:89703 )

♀ • homozygous mutant females reached senescence earlier than C57BL/6J wild-type mice

Genotype
MGI:3041263

hm2

• Allelic Composition: Nos3^tm1Weo/Nos3^tm1Weo
• Genetic Background: involves: 129

reproductive system

reproductive system phenotype ( J:73964 )

N • no difference in the number of oocytes released or survival of in vitro fertilized embryos is seen between female mutants on a 129/Sv background and 129/Sv wild-type mice

Genotype
MGI:2657241

hm3

• Allelic Composition: Nos3^tm1Weo/Nos3^tm1Weo
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

mortality/aging

postnatal lethality, incomplete penetrance ( J:51563 )

• fewer than the expected numbers of homozygotes are observed at 21 days of age

limbs/digits/tail

abnormal limb morphology ( J:51563 )

• 8-10% exhibit limb defects; defects range from meromelia to syndactyly

syndactyly ( J:51563 )

short limbs ( J:51563 )

• hypoplasia of the carpals, metacarpals, radius, and ulna

cardiovascular system

abnormal cardiac muscle relaxation ( J:51563 )

• aortic segments fail to relax in response to acetylcholine administration

muscle

abnormal cardiac muscle relaxation ( J:51563 )

• aortic segments fail to relax in response to acetylcholine administration

Genotype
MGI:5308985

cx4

• Allelic Composition: Asl^tm1Brle/Asl⁺; Nos3^tm1Weo/Nos3⁺
• Genetic Background: involves: 129S7/SvEvBrd

cardiovascular system

decreased systemic arterial diastolic blood pressure ( J:180208 )

• compared with Nos3^tm1Weo heterozygotes

decreased systemic arterial systolic blood pressure ( J:180208 )

• compared with Nos3^tm1Weo heterozygotes