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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gfi1tm1Tmo
targeted mutation 1, Tarik Moroy
MGI:2660761
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gfi1tm1Tmo/Gfi1tm1Tmo involves: 129S1/Sv * 129X1/SvJ MGI:5468268
hm2
 		Gfi1tm1Tmo/Gfi1tm1Tmo involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:2660763


Genotype
MGI:5468268
hm1
Allelic
Composition		 Gfi1tm1Tmo/Gfi1tm1Tmo
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gfi1tm1Tmo mutation (0 available); any Gfi1 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system

immune system

endocrine/exocrine glands




Genotype
MGI:2660763
hm2
Allelic
Composition		 Gfi1tm1Tmo/Gfi1tm1Tmo
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gfi1tm1Tmo mutation (0 available); any Gfi1 mutation (31 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:75143 )
• animals die between 2-3 months of age

growth/size/body
decreased body weight ( J:75143 )
• mutants weigh 10%-50% less than wild-type

hematopoietic system
abnormal T cell differentiation ( J:75143 )
• impaired pre-T cell development
abnormal granulocyte differentiation ( J:75143 )
• impaired granulocyte differentiation
abnormal monocyte differentiation ( J:75143 )
• accumulation of immature monocytes in the blood and bone marrow
decreased granulocyte number ( J:75143 )
• near complete absence of granulocytes in blood and bone marrow
decreased neutrophil cell number ( J:75143 )
• homozygotes are severely neutropenic
decreased B cell number ( J:75143 )
• decrease in frequency of B cells in lymph nodes and spleen
decreased T cell number ( J:75143 )
• decrease in frequency of mature T cells in lymph nodes and spleen
decreased thymocyte number ( J:75143 )
• thymic cell numbers are 10% that of wild-type

immune system
abnormal T cell differentiation ( J:75143 )
• impaired pre-T cell development
abnormal granulocyte differentiation ( J:75143 )
• impaired granulocyte differentiation
abnormal monocyte differentiation ( J:75143 )
• accumulation of immature monocytes in the blood and bone marrow
decreased granulocyte number ( J:75143 )
• near complete absence of granulocytes in blood and bone marrow
decreased neutrophil cell number ( J:75143 )
• homozygotes are severely neutropenic
decreased B cell number ( J:75143 )
• decrease in frequency of B cells in lymph nodes and spleen
decreased T cell number ( J:75143 )
• decrease in frequency of mature T cells in lymph nodes and spleen
decreased thymocyte number ( J:75143 )
• thymic cell numbers are 10% that of wild-type
increased interleukin-1 beta secretion ( J:75143 )
• increased IL-1beta production in response to low dose LPS challenge
increased interleukin-10 secretion ( J:75143 )
• increased IL-10 production in response to low dose LPS challenge
increased tumor necrosis factor secretion ( J:75143 )
• increased Tnf production in response to low dose LPS challenge
increased susceptibility to endotoxin shock ( J:75143 )
• increased inflammatory response when challenged with a low dose of LPS, animals died from endotoxic shock within 28 hours

hearing/vestibular/ear
abnormal cochlear inner hair cell morphology ( J:92833 )
• at E18.5, IHCs are clearly identified both at the base and the apex of the cochlea, but appear smaller and more immature relative to wild-type IHCs
abnormal inner hair cell stereociliary bundle morphology ( J:92833 )
• at E18.5, apical extensions, presumably primordia of stereociliary bundles, appear shorter and less organized
short inner hair cell stereocilia ( J:92833 )
• at E18.5, IHC stereociliary bundles appear shorter
abnormal cochlear outer hair cell morphology ( J:92833 )
• at E18.5, OHC morphology and patterning is markedly abnormal
abnormal outer hair cell stereociliary bundle morphology ( J:92833 )
• at E18.5, no intact OHC stereociliary bundles can be identified with phalloidin staining
• at E18.5 or P0, the luminal surface of OHCs throughout the length of the cochlea is composed of a sheath of poorly differentiated and disorganized apical projections and poorly defined cell boundaries
cochlear outer hair cell degeneration ( J:92833 )
• at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5
• in contrast, IHCs are readily identified along the entire length of the cochlea and the pillar cell row is undisrupted in the basal region
deafness ( J:92833 )
• homozygotes are deaf
abnormal vestibular system physiology ( J:92833 )
• homozygotes exhibit vestibular dysfunction due to hair cell loss

nervous system
abnormal cochlear inner hair cell morphology ( J:92833 )
• at E18.5, IHCs are clearly identified both at the base and the apex of the cochlea, but appear smaller and more immature relative to wild-type IHCs
abnormal inner hair cell stereociliary bundle morphology ( J:92833 )
• at E18.5, apical extensions, presumably primordia of stereociliary bundles, appear shorter and less organized
short inner hair cell stereocilia ( J:92833 )
• at E18.5, IHC stereociliary bundles appear shorter
abnormal cochlear outer hair cell morphology ( J:92833 )
• at E18.5, OHC morphology and patterning is markedly abnormal
abnormal outer hair cell stereociliary bundle morphology ( J:92833 )
• at E18.5, no intact OHC stereociliary bundles can be identified with phalloidin staining
• at E18.5 or P0, the luminal surface of OHCs throughout the length of the cochlea is composed of a sheath of poorly differentiated and disorganized apical projections and poorly defined cell boundaries
cochlear outer hair cell degeneration ( J:92833 )
• at E18.5, homozygotes exhibit OHC degeneration in the base and apex of the cochlea; less severe OHC degeneration is noted at E16.5
• in contrast, IHCs are readily identified along the entire length of the cochlea and the pillar cell row is undisrupted in the basal region

cellular
abnormal granulocyte differentiation ( J:75143 )
• impaired granulocyte differentiation
abnormal monocyte differentiation ( J:75143 )
• accumulation of immature monocytes in the blood and bone marrow

endocrine/exocrine glands
decreased thymocyte number ( J:75143 )
• thymic cell numbers are 10% that of wild-type




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory