Phenotypes associated with this allele

• Allele Symbol: Ripk2^tm1Flv
• Allele Name: targeted mutation 1, Richard A Flavell
• Allele ID: MGI:2660793
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ripk2^tm1Flv/Ripk2^tm1Flv	C.129S1(B6)-Ripk2^tm1Flv/Tvg	MGI:7764769
hm2	Ripk2^tm1Flv/Ripk2^tm1Flv	involves: 129S1/Sv	MGI:2661057
hm3	Ripk2^tm1Flv/Ripk2^tm1Flv	involves: 129S1/Sv * C57BL/6	MGI:3831401
cx4	Ripk2^tm1Flv/Ripk2^tm1Flv Tnip1^tm1.1Pcoh/Tnip1^tm1.1Pcoh	involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6	MGI:5296233

Genotype
MGI:7764769

hm1

• Allelic Composition: Ripk2^tm1Flv/Ripk2^tm1Flv
• Genetic Background: C.129S1(B6)-Ripk2^tm1Flv/Tvg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

decreased tumor incidence following infection ( J:329456 )

• specific-pathogen free (SPF) offspring from mice infected with Rauscher-like murine leukemia virus (MuLV infected offspring) exhibit increased latency and decreased total leukemia incidence compared to wild-type specific-pathogen free mice indicating that mice are more resistant to leukemia development

• however, mice do not show the same level of leukemia resistance that is seen in wild-type germ-free mice

Genotype
MGI:2661057

hm2

• Allelic Composition: Ripk2^tm1Flv/Ripk2^tm1Flv
• Genetic Background: involves: 129S1/Sv

immune system

decreased T cell proliferation ( J:75400 )

• reduced proliferation of thymocytes after Il-1beta stimulation

abnormal T-helper 1 physiology ( J:75400 )

• IFN-gamma production by T helper 1 cells reduced

• IFN-gamma production severely reduced after stimulation with either Il-18 or Il-12

abnormal cytokine secretion ( J:75400 )

decreased interleukin-6 secretion ( J:75400 )

• production by macrophages severely reduced after stimulation by ligands for some but not all Toll-like receptors

• production by thymocytes also reduced when stimulated by Il-1beta

decreased tumor necrosis factor secretion ( J:75400 )

• production by macrophages severely reduced after stimulation by ligands for some but not all Toll-like receptors

hematopoietic system

decreased T cell proliferation ( J:75400 )

• reduced proliferation of thymocytes after Il-1beta stimulation

abnormal T-helper 1 physiology ( J:75400 )

• IFN-gamma production by T helper 1 cells reduced

• IFN-gamma production severely reduced after stimulation with either Il-18 or Il-12

cellular

decreased T cell proliferation ( J:75400 )

• reduced proliferation of thymocytes after Il-1beta stimulation

Genotype
MGI:3831401

hm3

• Allelic Composition: Ripk2^tm1Flv/Ripk2^tm1Flv
• Genetic Background: involves: 129S1/Sv * C57BL/6

immune system

abnormal macrophage physiology ( J:132126 )

• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes

• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated

decreased interleukin-6 secretion ( J:132126 )

• bone marrow derived macrophages secrete half the amount of IL-6 as wild-type controls in response to culturing with L. monocytogenes

• IL-6 secretion is 22% that of controls when macrophages are pre-treated with LPS prior to L. monocytogenes incubation

• bone marrow derived macrophages fail to produce IL-6 when pre-treated with LPS prior to NOD2 stimulation

decreased tumor necrosis factor secretion ( J:132126 )

• bone marrow derived macrophages secrete 61% TNF as wild-type controls in response to culturing with L. monocytogenes

• IL-6 secretion is about 20% that of controls when macrophages are pre-treated with LPS or LTA prior to L. monocytogenes incubation

• bone marrow derived macrophages fail to produce TNF when pre-treated with LPS prior to NOD2 stimulation

hematopoietic system

abnormal macrophage physiology ( J:132126 )

• bone marrow derived macrophages produce less IL-6 and TNF when cultured in the presence of L. monocytogenes

• in macrophages pre-treated with TLR ligands (i.e. "tolerized"), macrophages produce significantly less inflammatory cytokines upon incubation with L. monocytogenes than controls that were LPS pre-treated

Genotype
MGI:5296233

cx4

• Allelic Composition: Ripk2^tm1Flv/Ripk2^tm1Flv; Tnip1^tm1.1Pcoh/Tnip1^tm1.1Pcoh
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6

immune system

enlarged spleen ( J:176826 )

enlarged lymph nodes ( J:176826 )

digestive/alimentary system

abnormal intestine morphology ( J:176826 )

• intestinal nodules

hematopoietic system

enlarged spleen ( J:176826 )

growth/size/body

enlarged spleen ( J:176826 )