Analysis Tools|
Allele Symbol Allele Name Allele ID |
Mpltm1Wsa targeted mutation 1, Warren S Alexander MGI:2660811 |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Summary |
14 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• plasma levels of CXCL4, CXCL5, and CXCL7 are attenuated compared to in wild-type mice
|
|
• plasma levels of CXCL4, CXCL5, and CXCL7 are attenuated compared to in wild-type mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mutant mice displayed a loss of megakaryocytes in the spleen and bone marrow
|
|
• mice are deficient in megakaryocyte progenitor cells
|
|
• mice had only 6% the number platelets detected in controls
|
|
• in clonal culture of bone marrow, total progenotor cell number was 38%- 44% of wildtype
• neutrophil-CFC, neutrophil-macrophage-CFC and pure erythroid-CFC were present at approximately 50% of normal levels
• cell reduction not seen in mature cell populations
|
| N |
• no alterations were detected in the ratios of B and T cells examined in the spleen, thymus and bone marrow using momoclonal antibodies
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• WBC is reduced by more than a third compared to Mpltm1Wsa homozygote controls
|
|
• there is a slight reduction in the number of red blood cells compared to Mpltm1Wsa homozygote controls
|
|
• platelet count is reduced by about half compared to Mpltm1Wsa homozygote controls
|
|
• WBC is reduced by more than a third compared to Mpltm1Wsa homozygote controls
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• platelet counts are significantly lower than single Mpl-deficient animals
|
|
• at lower end of normal range
|
|
• at lower end of normal range
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
|
|
• hematopoietic stem cell number were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
|
|
• megakaryocyte numbers were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
|
|
• thrombocytopenia of Mpltm1Wsa ameliorated, however platelet count is significantly lower than normal wild-type level
|
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
|
|
• megakaryocyte numbers were increased in the bone marrow compared to Mpltm1Wsa control, but not to the wild-type level
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• no homozygous Suz12Plt8Hiltanimals were found, indicating prenatal lethality
• embryos homozygous for Suz12Plt8Hilt were reduced in number at embryonic day 8.5
|
|
• embryos homozygous for Suz12Plt8Hilt were smaller than wild-type
|
|
• embryos homozygous for Suz12Plt8Hilt were smaller than wild-type
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
|
|
• thrombocytopenia of Mpltm1Wsa ameliorated, however platelet count is significantly lower than normal wild-type level
|
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa , increased platelet cell number was significant but not to the wild-type level
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice do not show leukopenia or anemia
|
|
• platelet counts are lower than single Mpl-deficient littermates at 7 weeks of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• lower than expected numbers of homozygotes born in matings of heterozygotes indicating a degree of perinatal or embryonic lethality
|
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
• numbers of most progenitor cell lines are elevated
|
|
• defective production of erythroid lineages
• large numbers of erythroid colony forming units but defective maturation
|
|
• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
|
|
• supraphysiological levels
|
|
• expanded red pulp with abnormal architecture
|
|
• abnormally small lymphoid follicles
|
|
• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
|
|
• expanded red pulp with abnormal architecture
|
|
• abnormally small lymphoid follicles
|
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
• numbers of most progenitor cell lines are unchanged
|
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa but not to wild-type
|
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
• numbers of most progenitor cell lines are elevated
|
|
• defective production of erythroid lineages
• large numbers of erythroid colony forming units but defective maturation
|
|
• supraphysiological levels
|
|
• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
|
|
• expanded red pulp with abnormal architecture
|
|
• abnormally small lymphoid follicles
|
|
• leukopenic
• significantly reduced numbers of B cells in bone marrow and spleen
|
|
• expanded red pulp with abnormal architecture
|
|
• abnormally small lymphoid follicles
|
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
• numbers of most progenitor cell lines are unchanged
|
|
• amelioration of thrombocytopenia compared to Mpltm1Wsa but not to wild-type
|
|
• increased numbers of progenitor cells and megakaryocytes in both bone marrow and in spleen
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• about 80% of mice fail to survive to weaning
|
|
• megakarycoyte numbers are about 10-fold higher in the bone marrow and spleen
|
|
• clonogenic myeloid progenitor cells are also significantly elevated by more than 3-fold in the spleen
|
|
• platelet count is increased more than 10-fold over Mpltm1Wsa homozygotes and more than twice as much as wild-type mice
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• the eosinophil number is almost double that of Mpltm1Wsa homozygotes and is similar to wild-type mice
|
|
• megakaryocyte numbers in the bone marrow and spleen are more than double what is found of wild-type mice
|
|
• clonogenic myeloid progenitor cells are also significantly elevated by more than 3-fold in the spleen
|
|
• the eosinophil number is almost double that of Mpltm1Wsa homozygotes and is similar to wild-type mice
|
|
• platelet count is increased more than 2-fold compared to Mpltm1Wsa homozygotes but is about half the number found in wild-type mice
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 11/12/2024 MGI 6.24 |
|
|
|
||
