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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Notch2tm1.1Hhi
targeted mutation 1.1, Hisamaru Hirai
MGI:2663710
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Cd19tm1(cre)Cgn/Cd19+
Notch2tm2Hhi/Notch2tm1.1Hhi 		involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:2663723
cn2
 		Cd19tm1(cre)Cgn/Cd19+
Notch2tm1.1Hhi/Notch2+ 		involves: 129P2/OlaHsd * C57BL/6 * CBA MGI:4360762
cn3
 		Notch2tm2Hhi/Notch2tm1.1Hhi
Tg(Mx1-cre)1Cgn/0 		involves: C57BL/6 * CBA MGI:2663720
cn4
 		Notch2tm1.1Hhi/Notch2tm1.1Hhi
Tg(Cd8a-cre)1Itan/0 		involves: C57BL/6 * CBA MGI:3851205


Genotype
MGI:2663723
cn1
Allelic
Composition		 Cd19tm1(cre)Cgn/Cd19+
Notch2tm2Hhi/Notch2tm1.1Hhi
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (11 available); any Cd19 mutation (60 available)
Notch2tm1.1Hhi mutation (1 available); any Notch2 mutation (99 available)
Notch2tm2Hhi mutation (1 available); any Notch2 mutation (99 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased transitional stage B cell number ( J:83476 )
• decrease in the number of T2 B cells
• almost complete absence of CD1d T2 B cells, the precursors of marginal zone B cells (pre-MZB)
abnormal spleen marginal zone morphology ( J:83476 )
• complete absence of the perifollicular rim consisting of IgMhiIgDlo cells in the spleen
absent marginal zone B cells ( J:83476 )
• near complete absence of marginal zone B cells in the spleen

immune system
decreased transitional stage B cell number ( J:83476 )
• decrease in the number of T2 B cells
• almost complete absence of CD1d T2 B cells, the precursors of marginal zone B cells (pre-MZB)
abnormal spleen marginal zone morphology ( J:83476 )
• complete absence of the perifollicular rim consisting of IgMhiIgDlo cells in the spleen
absent marginal zone B cells ( J:83476 )
• near complete absence of marginal zone B cells in the spleen




Genotype
MGI:4360762
cn2
Allelic
Composition		 Cd19tm1(cre)Cgn/Cd19+
Notch2tm1.1Hhi/Notch2+
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd19tm1(cre)Cgn mutation (11 available); any Cd19 mutation (60 available)
Notch2tm1.1Hhi mutation (1 available); any Notch2 mutation (99 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased marginal zone B cell number ( J:83476 )
• numbers of marginal zone B cells in the spleen are 1/6 to 1/4 of those in the control mice
• IgM+IgD- marginal zone B cells are reduced in the spleen

immune system
decreased marginal zone B cell number ( J:83476 )
• numbers of marginal zone B cells in the spleen are 1/6 to 1/4 of those in the control mice
• IgM+IgD- marginal zone B cells are reduced in the spleen




Genotype
MGI:2663720
cn3
Allelic
Composition		 Notch2tm2Hhi/Notch2tm1.1Hhi
Tg(Mx1-cre)1Cgn/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1.1Hhi mutation (1 available); any Notch2 mutation (99 available)
Notch2tm2Hhi mutation (1 available); any Notch2 mutation (99 available)
Tg(Mx1-cre)1Cgn mutation (7 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased marginal zone B cell number ( J:83476 )
• reduced number of marginal zone B cells in the spleen

immune system
immune system phenotype ( J:83476 )
N
• mutants exhibit normal T cell development
decreased marginal zone B cell number ( J:83476 )
• reduced number of marginal zone B cells in the spleen




Genotype
MGI:3851205
cn4
Allelic
Composition		 Notch2tm1.1Hhi/Notch2tm1.1Hhi
Tg(Cd8a-cre)1Itan/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Notch2tm1.1Hhi mutation (1 available); any Notch2 mutation (99 available)
Tg(Cd8a-cre)1Itan mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal CD8-positive, alpha-beta T cell physiology ( J:141009 )
• cells produce less granzyme B than control cells after stimulation with allogeneic control dendritic cells (DCs) or DL1-DCs
abnormal cytotoxic T cell physiology ( J:141009 )
• mice, injected with spleen cells that were pulsed with OT-I (ova) peptide and 4 days later given labeled spleen cells or labeled spleen cells that were pulsed with OT-I (ova) peptide show little CTL-mediated killing of either population; in contrast, control mice display preferential cytolysis of OT-I pulsed spleen cells
increased susceptibility to parasitic infection ( J:141009 )
• mice display much earlier death after Trypanosoma cruzi infection compared to controls

hematopoietic system
abnormal CD8-positive, alpha-beta T cell physiology ( J:141009 )
• cells produce less granzyme B than control cells after stimulation with allogeneic control dendritic cells (DCs) or DL1-DCs
abnormal cytotoxic T cell physiology ( J:141009 )
• mice, injected with spleen cells that were pulsed with OT-I (ova) peptide and 4 days later given labeled spleen cells or labeled spleen cells that were pulsed with OT-I (ova) peptide show little CTL-mediated killing of either population; in contrast, control mice display preferential cytolysis of OT-I pulsed spleen cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory