Phenotypes associated with this allele

• Allele Symbol: Fpr1^tm1Gao
• Allele Name: targeted mutation 1, Ji-Liang Gao
• Allele ID: MGI:2663726
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fpr1^tm1Gao/Fpr1^tm1Gao	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:2663727

Genotype
MGI:2663727

hm1

• Allelic Composition: Fpr1^tm1Gao/Fpr1^tm1Gao
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality ( J:53354 )

• in response to challenge with L. monocytogenes (2 10⁴ CFU), 73% of homozygotes die by day 4 compared with only 6% of wild-type controls

• only 6% of homozygotes survive beyond day 6, whereas 50% of wild-type controls remain healthy

immune system

abnormal innate immunity ( J:53354 )

• in response to challenge with Listeria monocytogenes (2 10⁴ CFU ), homozygotes exhibit an increased bacterial load in spleen and liver prior to the development of a specific cellular immune response, suggesting a defect in innate immunity

impaired neutrophil chemotaxis ( J:53354 )

• unlike wild-type mice, homozygotes fail to exhibit a fMLF (formyl-methionyl-leucyl-phenylalanine)-induced calcium flux in thioglycollate-elicited peritoneal (TP) neutrophils and PBMCs

• in vitro, mutant TP neutrophils fail to chemotax in response to fMLF, whereas wild-type neutrophils display a characteristic bell-shaped dose-response curve

• in vivo, homozygotes fail to display an increase in peripheral blood neutrophil concentrations at 90 min after s.c. injection with 200 l of 2 M fMLF, indicating absence of neutrophil mobilization

• no significant differences are observed in MIP-1alpha-induced chemotaxis between homozygous mutant and wild-type neutrophils

increased susceptibility to bacterial infection ( J:53354 )

• in response to challenge with L. monocytogenes (2 10⁴ CFU), homozygotes display an increased bacterial load (32- and 45-fold more bacteria in spleen and liver, respectively) at 2 days after infection relative to wild-type controls

increased susceptibility to bacterial infection induced morbidity/mortality ( J:53354 )

• in response to challenge with L. monocytogenes (2 10⁴ CFU), 73% of homozygotes die by day 4 compared with only 6% of wild-type controls

• only 6% of homozygotes survive beyond day 6, whereas 50% of wild-type controls remain healthy

cellular

impaired neutrophil chemotaxis ( J:53354 )

• unlike wild-type mice, homozygotes fail to exhibit a fMLF (formyl-methionyl-leucyl-phenylalanine)-induced calcium flux in thioglycollate-elicited peritoneal (TP) neutrophils and PBMCs

• in vitro, mutant TP neutrophils fail to chemotax in response to fMLF, whereas wild-type neutrophils display a characteristic bell-shaped dose-response curve

• in vivo, homozygotes fail to display an increase in peripheral blood neutrophil concentrations at 90 min after s.c. injection with 200 l of 2 M fMLF, indicating absence of neutrophil mobilization

• no significant differences are observed in MIP-1alpha-induced chemotaxis between homozygous mutant and wild-type neutrophils

hematopoietic system

impaired neutrophil chemotaxis ( J:53354 )

• unlike wild-type mice, homozygotes fail to exhibit a fMLF (formyl-methionyl-leucyl-phenylalanine)-induced calcium flux in thioglycollate-elicited peritoneal (TP) neutrophils and PBMCs

• in vitro, mutant TP neutrophils fail to chemotax in response to fMLF, whereas wild-type neutrophils display a characteristic bell-shaped dose-response curve

• in vivo, homozygotes fail to display an increase in peripheral blood neutrophil concentrations at 90 min after s.c. injection with 200 l of 2 M fMLF, indicating absence of neutrophil mobilization

• no significant differences are observed in MIP-1alpha-induced chemotaxis between homozygous mutant and wild-type neutrophils