Phenotypes associated with this allele

• Allele Symbol: Cd28^tm1Pjl
• Allele Name: targeted mutation 1, Philip J Lucas
• Allele ID: MGI:2664380
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd28^tm1Pjl/Cd28^tm1Pjl	involves: 129P2/OlaHsd * C57BL/6	MGI:2664944
cx2	Cd28^tm1Pjl/Cd28^tm1Pjl Tnfrsf9^tm1Byk/Tnfrsf9^tm1Byk	either: B6.Cg-Cd28^tm1Pjl Tnfrsf9^tm1Byk or C.Cg-Cd28^tm1Pjl Tnfrsf9^tm1Byk	MGI:2664937
cx3	Cd28^tm1Pjl/Cd28^tm1Pjl Tg(DO11.10)10Dlo/0	involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6	MGI:2664411
cx4	Cd28^tm1Pjl/Cd28^tm1Pjl Tg(H2-K-Cd86)27All/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3662645

Genotype
MGI:2664944

hm1

• Allelic Composition: Cd28^tm1Pjl/Cd28^tm1Pjl
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased splenocyte proliferation ( J:83722 )

• splenocytes show increased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

abnormal class switch recombination ( J:83722 )

• mutants are incapable of undergoing Ig class switch and fail to mount anti-DNP responses of IgG1, IgG2a and IgM

abnormal immunoglobulin level ( J:83722 )

decreased IgG1 level ( J:83722 )

• naive mutants exhibit decreased IgG1 levels and fail to mount anti-DNP responses of IgG1

decreased IgG2a level ( J:83722 )

• naive mutants exhibit decreased IgG2a levels and fail to mount anti-DNP responses of IgG2a

decreased IgM level ( J:83722 )

• mutants fail to mount anti-DNP responses of IgM and secrete reduced levels of IgM in response to a TD-antigen

abnormal cytotoxic T cell physiology ( J:83722 )

• Vesicular stomatitis virus-specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability is decreased in mutants

decreased susceptibility to type IV hypersensitivity reaction ( J:83722 )

• mutants exhibit significantly suppressed contact hypersensitivity to FITC and DNFB

decreased interleukin-12 secretion ( J:83722 )

• following ConA stimulation

hematopoietic system

increased splenocyte proliferation ( J:83722 )

• splenocytes show increased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

abnormal class switch recombination ( J:83722 )

• mutants are incapable of undergoing Ig class switch and fail to mount anti-DNP responses of IgG1, IgG2a and IgM

abnormal immunoglobulin level ( J:83722 )

decreased IgG1 level ( J:83722 )

• naive mutants exhibit decreased IgG1 levels and fail to mount anti-DNP responses of IgG1

decreased IgG2a level ( J:83722 )

• naive mutants exhibit decreased IgG2a levels and fail to mount anti-DNP responses of IgG2a

decreased IgM level ( J:83722 )

• mutants fail to mount anti-DNP responses of IgM and secrete reduced levels of IgM in response to a TD-antigen

abnormal cytotoxic T cell physiology ( J:83722 )

• Vesicular stomatitis virus-specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability is decreased in mutants

cellular

increased splenocyte proliferation ( J:83722 )

• splenocytes show increased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

Genotype
MGI:2664937

cx2

• Allelic Composition: Cd28^tm1Pjl/Cd28^tm1Pjl; Tnfrsf9^tm1Byk/Tnfrsf9^tm1Byk
• Genetic Background: either: B6.Cg-Cd28^tm1Pjl Tnfrsf9^tm1Byk or C.Cg-Cd28^tm1Pjl Tnfrsf9^tm1Byk

immune system

decreased splenocyte proliferation ( J:83722 )

• splenocytes show decreased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

abnormal class switch recombination ( J:83722 )

• mutants are incapable of undergoing Ig class switch and fail to mount anti-DNP responses of IgG1, IgG2a and IgM

decreased IgG1 level ( J:83722 )

• naive mutants exhibit decreased IgG1 levels and fail to mount anti-DNP responses of IgG1

decreased IgG2a level ( J:83722 )

• naive mutants exhibit decreased IgG2a levels and fail to mount anti-DNP responses of IgG2a

decreased IgM level ( J:83722 )

• mutants fail to mount anti-DNP responses of IgM and secrete reduced levels of IgM in response to a TD-antigen

abnormal cytotoxic T cell physiology ( J:83722 )

• Vesicular stomatitis virus-specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability is reduced

decreased susceptibility to type IV hypersensitivity reaction ( J:83722 )

• mutants exhibit significantly suppressed contact hypersensitivity to FITC and DNFB

decreased interleukin-12 secretion ( J:83722 )

• following ConA stimulation

hematopoietic system

decreased splenocyte proliferation ( J:83722 )

• splenocytes show decreased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

abnormal class switch recombination ( J:83722 )

• mutants are incapable of undergoing Ig class switch and fail to mount anti-DNP responses of IgG1, IgG2a and IgM

decreased IgG1 level ( J:83722 )

• naive mutants exhibit decreased IgG1 levels and fail to mount anti-DNP responses of IgG1

decreased IgG2a level ( J:83722 )

• naive mutants exhibit decreased IgG2a levels and fail to mount anti-DNP responses of IgG2a

decreased IgM level ( J:83722 )

• mutants fail to mount anti-DNP responses of IgM and secrete reduced levels of IgM in response to a TD-antigen

abnormal cytotoxic T cell physiology ( J:83722 )

• Vesicular stomatitis virus-specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability is reduced

cellular

decreased splenocyte proliferation ( J:83722 )

• splenocytes show decreased cellular proliferation compared to wild-type mice when stimulated with anti-CD3 or ConA

Genotype
MGI:2664411

cx3

• Allelic Composition: Cd28^tm1Pjl/Cd28^tm1Pjl; Tg(DO11.10)10Dlo/0
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C3H * C57BL/6

immune system

abnormal T cell activation ( J:25536 )

abnormal T cell proliferation ( J:25536 )

• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response

• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes

abnormal thymocyte activation ( J:25536 )

• thymocyte proliferative responses to OVA antigen are reduced

decreased interleukin-2 secretion ( J:25536 )

• 10- to 20-fold decrease in T cell IL-2 production when stimulated with OVA antigen

hematopoietic system

abnormal T cell activation ( J:25536 )

abnormal T cell proliferation ( J:25536 )

• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response

• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes

abnormal thymocyte activation ( J:25536 )

• thymocyte proliferative responses to OVA antigen are reduced

endocrine/exocrine glands

abnormal thymocyte activation ( J:25536 )

• thymocyte proliferative responses to OVA antigen are reduced

cellular

abnormal T cell proliferation ( J:25536 )

• naive T cells are able to initiate a response to antigen but show decreased sensitivity to lower antigen concentrations and are unable to maintain their proliferative response

• naive CD4+ antigen-specific T cell responses to mitogen (Con A) activation are reduced in splenocytes

Genotype
MGI:3662645

cx4

• Allelic Composition: Cd28^tm1Pjl/Cd28^tm1Pjl; Tg(H2-K-Cd86)27All/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

hematopoietic system

decreased B cell number ( J:112475 )

• transgenic mice deficient in Cd28 express normal numbers of peripheral B lymphocytes in contrast to Tg(H2-K-Cd86)27All mice

immune system

decreased B cell number ( J:112475 )

• transgenic mice deficient in Cd28 express normal numbers of peripheral B lymphocytes in contrast to Tg(H2-K-Cd86)27All mice