Phenotypes associated with this allele

• Allele Symbol: Slc8a2^tm1(cre)Hssh
• Allele Name: targeted mutation 1, Hee-Sup Shin
• Allele ID: MGI:2667136
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc8a2^tm1(cre)Hssh/Slc8a2^tm1(cre)Hssh	involves: 129 * C57BL/6J	MGI:2667137

Genotype
MGI:2667137

hm1

• Allelic Composition: Slc8a2^tm1(cre)Hssh/Slc8a2^tm1(cre)Hssh
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

enhanced contextual conditioning behavior ( J:84050 )

• mutants exhibit more freezing behavior than wild-type mice when returned to the same shock chamber 24 hours after the training for fear memory, indicating enhanced long-term memory for contextual fear conditioning

• however, no differences seen in the cued fear conditioning assay

abnormal object recognition memory ( J:84050 )

• mutants exhibit enhanced object recognition memory; at 24 hours, but not at 1 hour retention interval, mutants show increased preference for the novel object compared to wild-type mice

abnormal spatial learning ( J:84050 )

• mutants show shorter escape latencies than wild-type mice in the Morris water maze test, indicating enhanced spatial learning

• enhanced spatial learning is also seen in the first probe test after the third day session, with mutants spending more time in the target quadrant than wild-type mice

• on the second probe test after the seventh day sessions, wild-type and mutants reach the same level of learning and memory

nervous system

abnormal neuron physiology ( J:84050 )

• hippocampal neurons exhibit a significant delay in clearance of elevated calcium following depolarization

abnormal synaptic plasticity ( J:84050 )

• mutants exhibit enhanced presynaptic short-term plasticity

abnormal CNS synaptic transmission ( J:84050 )

• frequency threshold for LTP and LTD in the hippocampal CA1 region is shifted to a lowered stimulus frequency

enhanced long-term potentiation ( J:84050 )

• administration of a single tetanus at 100 Hz, 50 Hz, or 10 Hz elicits an enhanced LTP in the mutant compared to wild-type

abnormal post-tetanic potentiation ( J:84050 )

• in the presence of D-AP5 to block NMDA receptors, mutants exhibit a higher peak of post-tetanic potentiation in response to a single 100 Hz tetanus stimulation than wild-type

abnormal long-term depression ( J:84050 )

• stimulation at 1 Hz induces a depression of synaptic transmission within 10 min in both 3-4 week old wild-type and mutant mice, however the depression is not maintained in the mutant and instead, potentiation is developed over time

• stimulations at 0.2 Hz produce long term depression in the mutant unlike in wild-type mice

enhanced paired-pulse facilitation ( J:84050 )

• paired-pulse facilitation is enhanced compared to wild-type at interpulse intervals below 100 ms