Phenotypes associated with this allele

• Allele Symbol: Drap1^tm1Mms
• Allele Name: targeted mutation 1, Michael M Shen
• Allele ID: MGI:2667159
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Drap1^tm1Mms/Drap1^tm1Mms	either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J)	MGI:2667161
cx2	Drap1^tm1Mms/Drap1^tm1Mms Nodal^tm1Rob/Nodal^+	involves: 129S/SvEv * 129S6/SvEvTac	MGI:3850317

Genotype
MGI:2667161

hm1

• Allelic Composition: Drap1^tm1Mms/Drap1^tm1Mms
• Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) or (involves: 129S6/SvEvTac * C57BL/6J)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:80667 )

• no viable homozygotes are recovered from the progeny of heterozygous intercrosses

• however, homozygotes are recovered at normal Mendelian ratios with no detectable abnormalities up to E6.5

embryo

abnormal gastrulation ( J:80667 )

• at E7.0, homozygotes show severe gastrulation defects, including a caudal bulge that corresponds to a significantly expanded primitive streak

• gastrulation defects are associated with increased expression of Nodal and can be partially suppressed by Nodal heterozygosity

abnormal gastrulation movements ( J:80667 )

• at E7.0, homozygotes display little or no mesoderm migration circumferentially to the rostral side, with only limited movement observed anteriorly or extraembryonically

abnormal mesoderm development ( J:80667 )

• at E7.5, homozygotes display absence of mesoderm on the rostral side

• analysis of markers of nascent mesoderm indicates altered regional specification resulting in an expansion of axial mesendoderm at the expense of paraxial, lateral, and extraembryonic mesoderm

abnormal mesendoderm development ( J:80667 )

• at E7.5, homozygotes exhibit limited anterior migration of axial mesendoderm

increased axial mesoderm size ( J:80667 )

• homozygotes display expansion of the axial mesoderm

absent paraxial mesoderm ( J:80667 )

• homozygotes fail to specify paraxial mesoderm

abnormal primitive streak formation ( J:80667 )

• at E7.5, homozygotes display abnormal primitive streak morphology, with nascent mesodermal cells retained within or near a greatly expanded primitive streak

Genotype
MGI:3850317

cx2

• Allelic Composition: Drap1^tm1Mms/Drap1^tm1Mms; Nodal^tm1Rob/Nodal⁺
• Genetic Background: involves: 129S/SvEv * 129S6/SvEvTac

mortality/aging

embryonic lethality, complete penetrance ( J:80667 )

• mutant embryos die by E9.5

embryo

abnormal gastrulation ( J:80667 )

• mutant embryos are partially rescued from the mesoderm defects observed in Drap1^tm1Mms homozygotes, but are still unable to complete gastrulation

abnormal primitive streak formation ( J:80667 )

• at E7.5 and E8.25, mutant embryos display a characteristic proximal bulge in the caudal primitive streak