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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Map2k2tm1Chrn
targeted mutation 1, Jean Charron
MGI:2668345
Summary 10 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Map2k2tm1Chrn/Map2k2tm1Chrn involves: 129/Sv * C57BL/6J * SJL MGI:2674996
cn2
 		Hivep3tm3Glm/Hivep3+
Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(Prrx1-cre)1Cjt/0 		involves: 129 * C57BL/6J * SJL * SJL/J MGI:5550510
cn3
 		Krastm1Bbd/Kras+
Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn 		involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL MGI:5508244
cn4
 		Krastm1Bbd/Kras+
Map2k2tm1Chrn/Map2k2tm1Chrn 		involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL MGI:5508243
cn5
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(Cyp17a1-icre)AJako/0 		involves: 129/Sv * C57BL/6 * C57BL/6J * SJL MGI:6856826
cn6
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
H2az2Tg(Wnt1-cre)11Rth/H2az2+ 		involves: 129/Sv * C57BL/6J * CBA/J * SJL MGI:5659952
cn7
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-cre/ERT2)1Ipc/? 		involves: 129/Sv * C57BL/6J * SJL MGI:3714929
cn8
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Polr2atm1(cre/ERT2)Bbd/Polr2atm1(cre/ERT2)Bbd 		involves: 129/Sv * C57BL/6J * SJL MGI:5508246
cn9
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-cre)1Efu/? 		involves: 129/Sv * C57BL/6J * SJL MGI:3714928
cx10
 		Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-RAF1/ESR1)1Pkha/? 		involves: 129/Sv * C57BL/6 * C57BL/6J * SJL MGI:3714930


Genotype
MGI:2674996
hm1
Allelic
Composition		 Map2k2tm1Chrn/Map2k2tm1Chrn
Genetic
Background		 involves: 129/Sv * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
normal phenotype
no abnormal phenotype detected ( J:84382 )
• apparently function can be compensated for by Map2k1




Genotype
MGI:5550510
cn2
Allelic
Composition		 Hivep3tm3Glm/Hivep3+
Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(Prrx1-cre)1Cjt/0
Genetic
Background		 involves: 129 * C57BL/6J * SJL * SJL/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Hivep3tm3Glm mutation (0 available); any Hivep3 mutation (104 available)
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Tg(Prrx1-cre)1Cjt mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton




Genotype
MGI:5508244
cn3
Allelic
Composition		 Krastm1Bbd/Kras+
Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Genetic
Background		 involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm1Bbd mutation (2 available); any Kras mutation (84 available)
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:172200 )
• Ad-Cre treated mice show 50% survival at 57 weeks of age compared to 33 weeks of age in single Kras heterozygous controls, indicating an almost 100% increase in survival

neoplasm
decreased classified tumor incidence ( J:172200 )
• 6 months following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre), only a few tumors are observed; tumors that are present carry unrecombined Map2k1 alleles and express normal levels of Map2k1




Genotype
MGI:5508243
cn4
Allelic
Composition		 Krastm1Bbd/Kras+
Map2k2tm1Chrn/Map2k2tm1Chrn
Genetic
Background		 involves: 129/Sv * 129S1/Sv * 129X1/SvJ * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Krastm1Bbd mutation (2 available); any Kras mutation (84 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:172200 )
• Ad-Cre treated mutants show 50% survival at 52 weeks compared to 45 weeks in single Kras heterozygous controls, indicating a slight 19% increase in survival

neoplasm
increased lung non-small cell carcinoma incidence ( J:172200 )
• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes

respiratory system
increased lung non-small cell carcinoma incidence ( J:172200 )
• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes




Genotype
MGI:6856826
cn5
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(Cyp17a1-icre)AJako/0
Genetic
Background		 involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Tg(Cyp17a1-icre)AJako mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:278979 )
N
• males show no significant differences in testis weight, seminiferous tubule diameter, seminiferous epithelium morphology, expression of Sertoli and germ cell markers, total epididymal sperm count or sperm motility relative to control males
decreased seminal vesicle weight ( J:278979 )
• seminal vesicle weight is significantly reduced at P75 and P180
decreased Leydig cell number ( J:278979 )
• although an increase in Leydig cell numbers is observed up to ~P35, the total number of Leydig cells (Cyp11a1 positive cells) per testis is significantly reduced at all ages examined
• Leydig cell hypoplasia is accompanied by reduced testicular expression of several Leydig cell markers
decreased testes secretion ( J:278979 )
• in culture, primary Leydig cells from 50-day-old males show a drastic reduction in basal testosterone as well as severely impaired testosterone synthesis in response to stimulation with hCG (human choriogonadotropin), dibutyryl(Bt2)cAMP (a permeable cAMP analog), 22OHC (22-hydroxycholesterol) or P5 (pregnenolone)
• however, cAMP accumulation in response to hCG stimulation is normal
decreased litter size ( J:278979 )
• when males are paired with females of known fertility, the average number of pups/littler is significantly lower than that for control males (3.3 +/- 1.9 versus 6.5 +/- 0.6, respectively)
reduced male fertility ( J:278979 )
• male fertility starts to decline at ~3 months of age, and by 6 months the cumulative number of pups sired by males paired with fertile females is only ~50% of that sired by control males
• at the end of the 6-month mating period, the number of litters and number of pups/litter are significantly reduced relative to controls from 5.7 +/- 0.2 to 3.8 +/- 0.6 and from 6.2 +/- 0.3 to 4.8 +/- 0.5, respectively, while the number of days between litters is increased from 27 +/- 1 to 31 +/- 1

homeostasis/metabolism
abnormal circulating androgen level ( J:278979 )
• at P180, males exhibit several signs of hypoandrogenemia, including increased serum LH levels, reduced expression of two renal androgen-responsive genes, and decreased seminal vesicle weight relative to control males
increased circulating luteinizing hormone level ( J:278979 )
• serum LH levels are significantly increased at P180
abnormal testosterone level ( J:278979 )
• testicular extracts from 180-day-old males show a~40% reduction in intratesticular testosterone levels relative to controls

endocrine/exocrine glands
decreased seminal vesicle weight ( J:278979 )
• seminal vesicle weight is significantly reduced at P75 and P180
decreased Leydig cell number ( J:278979 )
• although an increase in Leydig cell numbers is observed up to ~P35, the total number of Leydig cells (Cyp11a1 positive cells) per testis is significantly reduced at all ages examined
• Leydig cell hypoplasia is accompanied by reduced testicular expression of several Leydig cell markers
decreased testes secretion ( J:278979 )
• in culture, primary Leydig cells from 50-day-old males show a drastic reduction in basal testosterone as well as severely impaired testosterone synthesis in response to stimulation with hCG (human choriogonadotropin), dibutyryl(Bt2)cAMP (a permeable cAMP analog), 22OHC (22-hydroxycholesterol) or P5 (pregnenolone)
• however, cAMP accumulation in response to hCG stimulation is normal

behavior/neurological
decreased copulatory plug deposition ( J:278979 )
• when males are paired with females of known fertility, the number of vaginal plugs is significantly lower than that detected with control males




Genotype
MGI:5659952
cn6
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
H2az2Tg(Wnt1-cre)11Rth/H2az2+
Genetic
Background		 involves: 129/Sv * C57BL/6J * CBA/J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2az2Tg(Wnt1-cre)11Rth mutation (2 available); any H2az2 mutation (26 available)
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
craniofacial
mandible hypoplasia ( J:144862 )
• at E17.5
short maxilla ( J:144862 )
• at E17.5

cardiovascular system
persistent truncus arteriosus ( J:144862 )
• at E16.5 and E17.5

hearing/vestibular/ear

growth/size/body
absent tongue ( J:144862 )
abnormal outer ear morphology ( J:144862 )

digestive/alimentary system

skeleton
mandible hypoplasia ( J:144862 )
• at E17.5
short maxilla ( J:144862 )
• at E17.5

vision/eye




Genotype
MGI:3714929
cn7
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-cre/ERT2)1Ipc/?
Genetic
Background		 involves: 129/Sv * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Tg(KRT14-cre/ERT2)1Ipc mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal tongue epithelium morphology ( J:122484 )
• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death

craniofacial
abnormal tongue epithelium morphology ( J:122484 )
• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death

integument
skin hypoplasia ( J:122484 )
• following tamoxifen treatment, mice have increased epidermal hypoplasia and increased cell death

growth/size/body
abnormal tongue epithelium morphology ( J:122484 )
• following tamoxifen treatment, mice have increased tongue epidermal hypoplasia and increased cell death




Genotype
MGI:5508246
cn8
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Polr2atm1(cre/ERT2)Bbd/Polr2atm1(cre/ERT2)Bbd
Genetic
Background		 involves: 129/Sv * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Polr2atm1(cre/ERT2)Bbd mutation (3 available); any Polr2a mutation (92 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:172200 )
• mice fed a tamoxifen diet at 30 days of age show rapid deterioration of their health and die 2 weeks after starting the tamoxifen diet

digestive/alimentary system
abnormal intestine morphology ( J:172200 )
• mice fed a tamoxifen diet exhibit severe alterations in the structure of intestinal and colonic tissue
abnormal crypts of Lieberkuhn morphology ( J:172200 )
• severe shortening of crypts in the colon of tamoxifen fed mice

endocrine/exocrine glands
abnormal crypts of Lieberkuhn morphology ( J:172200 )
• severe shortening of crypts in the colon of tamoxifen fed mice




Genotype
MGI:3714928
cn9
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-cre)1Efu/?
Genetic
Background		 involves: 129/Sv * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Tg(KRT14-cre)1Efu mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
neonatal lethality, incomplete penetrance ( J:122484 )
• the majority of mice die within 24 hours of birth

digestive/alimentary system
abnormal tongue epithelium morphology ( J:122484 )
• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered

growth/size/body
abnormal tongue epithelium morphology ( J:122484 )
• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered
abnormal outer ear morphology ( J:122484 )
• ear flaps are detached
decreased body size ( J:122484 )
• mice are smaller at birth
decreased body weight ( J:122484 )
• mice lose 4%-10% of birth weight within 6 hours

hearing/vestibular/ear
abnormal outer ear morphology ( J:122484 )
• ear flaps are detached

vision/eye
eyelids open at birth ( J:122484 )
• in some mice

homeostasis/metabolism
impaired skin barrier function ( J:122484 )
• mice lose 4%-10% of birth weight within 6 hours and dye is more readily absorbed than in wild-type mice

craniofacial
abnormal tongue epithelium morphology ( J:122484 )
• mice display full-thickness epithelium death and detachment from the underlying tissue
• however, suckling behavior was not altered
abnormal outer ear morphology ( J:122484 )
• ear flaps are detached

integument
increased keratinocyte apoptosis ( J:122484 )
• at E17.5, keratinocytes in the basal layer but not in the hair follicles undergo increased apoptosis compared to normal
impaired skin barrier function ( J:122484 )
• mice lose 4%-10% of birth weight within 6 hours and dye is more readily absorbed than in wild-type mice
decreased hair follicle number ( J:122484 )
• fewer hair follicles are present including 37% fewer peleage follicles
abnormal vibrissa follicle morphology ( J:122484 )
• 90% fewer vibrissae follicles compared to wild-type mice
decreased hair follicle cell proliferation ( J:122484 )
• hair follicles undergo reduced proliferation
skin hypoplasia ( J:122484 )
• epidermis is hypoplastic

cellular
increased keratinocyte apoptosis ( J:122484 )
• at E17.5, keratinocytes in the basal layer but not in the hair follicles undergo increased apoptosis compared to normal




Genotype
MGI:3714930
cx10
Allelic
Composition		 Map2k1tm1Chrn/Map2k1tm1Chrn
Map2k2tm1Chrn/Map2k2tm1Chrn
Tg(KRT14-RAF1/ESR1)1Pkha/?
Genetic
Background		 involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Map2k1tm1Chrn mutation (1 available); any Map2k1 mutation (94 available)
Map2k2tm1Chrn mutation (1 available); any Map2k2 mutation (37 available)
Tg(KRT14-RAF1/ESR1)1Pkha mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
skin hypoplasia ( J:122484 )
• following tamoxifen treatment, mice have increased epidermal hypoplasia and increased cell death




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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory