Phenotypes associated with this allele

• Allele Symbol: Tnfsf14^tm1Ddy
• Allele Name: targeted mutation 1, Derek D Yang
• Allele ID: MGI:2668383
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tnfsf14^tm1Ddy/Tnfsf14^tm1Ddy	involves: 129S1/Sv * 129X1/SvJ	MGI:2675258

Genotype
MGI:2675258

hm1

• Allelic Composition: Tnfsf14^tm1Ddy/Tnfsf14^tm1Ddy
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:84366 )

N • homozygotes exhibit normal lymphoid tissue morphology with normal lymphoid cell populations in bone marrow, lymph node and spleen relative to wild-type controls

abnormal cytotoxic T cell physiology ( J:84366 )

• in culture, total splenocytes from homozygous mutant mice display significantly decreased CTL activity against allogeneic P815 mastocytoma (H-2^d) target cells relative to wild-type splenocytes

• however, purified mutant CD8⁺ effector cells exhibit normal CTL activity against P815 cells on a per cell basis relative to wild-type CD8⁺ cells

abnormal T cell activation ( J:84366 )

• upon activation with anti-CD3 and anti-CD28, mutant CD8⁺ T cells exhibit severely reduced cell-surface expression levels of IL-2 receptor (CD25) relative to wild-type cells, suggesting impaired IL-2 signaling

• addition of exogenous IL-12 increases CD25 cell-surface levels on mutant CD8⁺ T cells to a lesser extent than in wild-type CD8⁺ T cells (68.9 +/- 10.4% vs 90.6 +/- 1.6%, respectively)

decreased T cell proliferation ( J:84366 )

• homozygotes display significantly reduced CD8⁺ (but not CD4⁺) T cell proliferation in response to TCR or TCR plus CD28-mediated stimulation, by determined by [³H]thymidine incorporation

• in a mixed lymphocyte reaction (MLR) assay, purified mutant CD8⁺ T cells display significantly reduced proliferation in response to allogeneic P815 mastocytoma (H-2^d) stimulator cells

• impaired CD8⁺ T cell proliferation is at least partly due to defects in cell division progression, in the absence of increased CD8⁺ T cell apoptosis

• following activation by anti-CD3 and anti-CD28, mutant CD8⁺ T cells show a significantly reduced proliferative response to exogenous rmIL-2 relative to wild-type cells

• addition of exogenous IL-12 increases cell-surface expression of CD25 on mutant CD8⁺ T cells but fails to ameliorate the proliferative defect

hematopoietic system

abnormal cytotoxic T cell physiology ( J:84366 )

• in culture, total splenocytes from homozygous mutant mice display significantly decreased CTL activity against allogeneic P815 mastocytoma (H-2^d) target cells relative to wild-type splenocytes

• however, purified mutant CD8⁺ effector cells exhibit normal CTL activity against P815 cells on a per cell basis relative to wild-type CD8⁺ cells

abnormal T cell activation ( J:84366 )

• upon activation with anti-CD3 and anti-CD28, mutant CD8⁺ T cells exhibit severely reduced cell-surface expression levels of IL-2 receptor (CD25) relative to wild-type cells, suggesting impaired IL-2 signaling

• addition of exogenous IL-12 increases CD25 cell-surface levels on mutant CD8⁺ T cells to a lesser extent than in wild-type CD8⁺ T cells (68.9 +/- 10.4% vs 90.6 +/- 1.6%, respectively)

decreased T cell proliferation ( J:84366 )

• homozygotes display significantly reduced CD8⁺ (but not CD4⁺) T cell proliferation in response to TCR or TCR plus CD28-mediated stimulation, by determined by [³H]thymidine incorporation

• in a mixed lymphocyte reaction (MLR) assay, purified mutant CD8⁺ T cells display significantly reduced proliferation in response to allogeneic P815 mastocytoma (H-2^d) stimulator cells

• impaired CD8⁺ T cell proliferation is at least partly due to defects in cell division progression, in the absence of increased CD8⁺ T cell apoptosis

• following activation by anti-CD3 and anti-CD28, mutant CD8⁺ T cells show a significantly reduced proliferative response to exogenous rmIL-2 relative to wild-type cells

• addition of exogenous IL-12 increases cell-surface expression of CD25 on mutant CD8⁺ T cells but fails to ameliorate the proliferative defect

cellular

decreased T cell proliferation ( J:84366 )

• homozygotes display significantly reduced CD8⁺ (but not CD4⁺) T cell proliferation in response to TCR or TCR plus CD28-mediated stimulation, by determined by [³H]thymidine incorporation

• in a mixed lymphocyte reaction (MLR) assay, purified mutant CD8⁺ T cells display significantly reduced proliferation in response to allogeneic P815 mastocytoma (H-2^d) stimulator cells

• impaired CD8⁺ T cell proliferation is at least partly due to defects in cell division progression, in the absence of increased CD8⁺ T cell apoptosis

• following activation by anti-CD3 and anti-CD28, mutant CD8⁺ T cells show a significantly reduced proliferative response to exogenous rmIL-2 relative to wild-type cells

• addition of exogenous IL-12 increases cell-surface expression of CD25 on mutant CD8⁺ T cells but fails to ameliorate the proliferative defect