Phenotypes associated with this allele

• Allele Symbol: Sema3f^tm1.2Ddg
• Allele Name: targeted mutation 1.2, David D Ginty
• Allele ID: MGI:2670663
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg	Not Specified	MGI:2670678
cn2	Sema3f^tm1.1Ddg/Sema3f^tm1.2Ddg Tg(Syn1-cre)671Jxm/0	involves: C57BL/6 * CBA	MGI:2670688
cx3	Nrp2^tm1.2Mom/Nrp2^+ Sema3f^tm1.2Ddg/Sema3f^+	involves: 129P2/OlaHsd	MGI:5897107
cx4	Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg Sema3g^tm1(KOMP)Vlcg/Sema3g^tm1(KOMP)Vlcg	involves: C57BL/6NTac	MGI:5897108

Genotype
MGI:2670678

hm1

• Allelic Composition: Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal lymphatic vessel morphology ( J:226501 )

• increased lymphatic branching complexity, less so than in Sema3g^{tm1(KOMP)Vlcg} homozygotes

abnormal lymphatic vessel endothelial cell morphology ( J:226501 )

• increased numbers

abnormal lymphatic system physiology ( J:226501 )

• increased lymphatic endothelial cell proliferation at E14.5

nervous system

abnormal axon fasciculation ( J:84687 )

• oculomotor nerve, anterior commissure, fasciculus retroflexus, and stria terminalis are defasciculated

abnormal axon guidance ( J:84687 )

• defects in CNS axon pathfinding

• improper guidance of amygdalar efferents to their destinations in the hypothalamus and bed nucleus of the stria terminalis

• axons of the infrapyramidal tract extend abnormally into the stratum oriens of CA3

• the anterior commissure fails to decussate normally

abnormal hippocampus development ( J:84687 )

• improper development of the infrapyramidal tract

abnormal habenula morphology ( J:84687 )

• projections from the medial habenula to the interpeduncular nucleus are defasciculated

abnormal innervation ( J:84687 )

• abnormal limbic projections in the forebrain, midbrain and hippocampus

abnormal nervous system tract morphology ( J:84687 )

• 4 week old mice exhibit aberrantly targeted hippocampal infrapyramidal tract with axons of the infrapyramidal tract extending into the stratum oriens of CA3

• improper targeting of the stria terminalis fibers to the hypothalamus and the bed nucleus of the stria terminalis

abnormal anterior commissure morphology ( J:84687 )

• the anterior commissure is defasciculated and fails to decussate normally

abnormal oculomotor nerve morphology ( J:84687 )

• severely defasciculated, but maintains a peripheral trajectory

absent trochlear nerve ( J:84687 )

• trochlear nerve is largely absent and only a few axons exit the hindbrain-midbrain junction

growth/size/body

decreased body size ( J:84687 )

• body size is initially smaller but mutants achieve normal size by 3 months of age

cellular

abnormal axon fasciculation ( J:84687 )

• oculomotor nerve, anterior commissure, fasciculus retroflexus, and stria terminalis are defasciculated

abnormal axon guidance ( J:84687 )

• defects in CNS axon pathfinding

• improper guidance of amygdalar efferents to their destinations in the hypothalamus and bed nucleus of the stria terminalis

• axons of the infrapyramidal tract extend abnormally into the stratum oriens of CA3

• the anterior commissure fails to decussate normally

Genotype
MGI:2670688

cn2

• Allelic Composition: Sema3f^tm1.1Ddg/Sema3f^tm1.2Ddg; Tg(Syn1-cre)671Jxm/0
• Genetic Background: involves: C57BL/6 * CBA

nervous system

abnormal axon fasciculation ( J:84687 )

• the anterior limb of the anterior commissure is defasciculated

abnormal hippocampus development ( J:84687 )

• 2 of 3 mutants exhibit improper development of the infrapyramidal tract

abnormal innervation ( J:84687 )

• most axons of the anterior limb of the anterior commissure cross the midline aberrantly and axons of the infrapyramidal tract extend beyond the stratum oriens of CA3

• however, no defects in stria terminalis targeting

abnormal nervous system tract morphology ( J:84687 )

• 2 of 3 mutants exhibit an infrapyramidal tract defect in which axons extend far into the stratum oriens of CA3, beyond the level at which they normally turn dorsally into the stratum radiatum

abnormal anterior commissure morphology ( J:84687 )

• the anterior limb of the anterior commissure is reduced and defasciculated

• although a small number of axons of the anterior commissure decussate properly, most axons cross the midline aberrantly as smaller tightly bundled fascicles

cellular

abnormal axon fasciculation ( J:84687 )

• the anterior limb of the anterior commissure is defasciculated

Genotype
MGI:5897107

cx3

• Allelic Composition: Nrp2^tm1.2Mom/Nrp2⁺; Sema3f^tm1.2Ddg/Sema3f⁺
• Genetic Background: involves: 129P2/OlaHsd

immune system

abnormal lymphatic vessel endothelial cell morphology ( J:226501 )

• increased numbers as much as in Sema3f^tm1.2Ddg homozygotes

Genotype
MGI:5897108

cx4

• Allelic Composition: Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg; Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6NTac

immune system

abnormal lymphatic vessel morphology ( J:226501 )

• synergistic increased lymphatic branching complexity

abnormal lymphatic vessel endothelial cell morphology ( J:226501 )

• increased numbers similar to in Sema3f^tm1.2Ddg homozygotes

• however, lymphatic endothelial cell progenitors exhibit normal migration

lymphatic vessel hyperplasia ( J:226501 )

• hyperplastic lymphatic vasculature in the back skin at E15.5

• however, defective back lymphatic network appears normal from E17.5 onward