All Phenotypes Mapt<tm1(EGFP)Klt> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt	B6.129S4-Mapt^tm1(EGFP)Klt	MGI:5604725
hm2	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt	involves: 129S4/SvJae * C57BL/6	MGI:2670770
cx3	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0	B6.Cg-Tg(MAPT)8cPdav Mapt^tm1(EGFP)Klt/J	MGI:5008417
cx4	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(Thy1-MAPT*)30Schd/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:4940110
cx5	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/?	involves: 129S4/SvJae * C57BL/6 * Swiss Webster	MGI:3663749

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
Genetic Background	B6.129S4-Mapt^tm1(EGFP)Klt

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (430 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:213115 )

• 6 month old male mice freeze less during contextual fear conditioning as compared to wild-type

impaired cued conditioning behavior ( J:213115 )

• 6 month old male mice freeze less during cued fear conditioning as compared to wild-type

abnormal motor learning ( J:213115 )

• no improvement in motor skills or learning is observed in 6 month old male mice on accelerating rotarod as compared to wild-type

• however, latency to fall is similar to wild-type

enhanced spatial learning ( J:213115 )

• improved performance in spatial navigation (escape latency and path length)is observed in 6 month old male mice, but only in the second week (days 6-10) of Morris water maze testing

nervous system

reduced long-term potentiation ( J:213115 )

• mice display a brief episode of potentiation but fail to develop long term potentiation following 3 repetitive theta bursts

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt
Genetic Background	involves: 129S4/SvJae * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (430 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:66561 )

• homozygotes are viable and fertile with no detectable abnormalities in morphology of the central or peripheral nervous systems

behavior/neurological

behavior/neurological phenotype ( J:169074 )

N	• mice show no significant motor deficits up to 12 months of age • spatial working memory is not impaired

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0
Genetic Background	B6.Cg-Tg(MAPT)8cPdav Mapt^tm1(EGFP)Klt/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (430 available)
Tg(MAPT)8cPdav mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Beta-amyloid plaques in Tg(APPswe,PSEN1dE9)85Dbo/0 mice and neurofibrillary tangles in Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0 mice

behavior/neurological

abnormal spatial learning ( J:172426 )

• 6 month old mutants exhibit slower visuospatial learning than control mice

• in the visuospatial re-learning test performed at 7, 8, 9, and 15 months of age, mutants exhibit a decrease in the speed of re-learning the task compared to controls

• however, mutants perform better than Tg(APPswe,PSEN1dE9)85Dbo mice on the visuospatial re-learning test at 9 and 18 months of age

taste/olfaction

taste/olfaction phenotype ( J:172426 )

N	• mutants do not exhibit Alzheimer's disease-related impairment in olfactory performance in tests based on an operant conditioning procedure and in tests based on a habituation/dishabituation procedure

nervous system

neurofibrillary tangles ( J:172426 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:172426

Allelic Composition	Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(Thy1-MAPT*)30Schd/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mapt^tm1(EGFP)Klt mutation (4 available); any Mapt mutation (430 available)
Tg(Thy1-MAPT*)30Schd mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:169074 )

• mice show reduced survival relative to wild-type, with almost 100% mortality observed by 16 months

• accelerated mortality is observed before 3 months of age (after 1 month of age) compared to wild-type or Tg(Thy1-MAPT*)30Schd single mutant animals

nervous system

nervous system phenotype ( J:169074 )

N	• at 12 months, neuronal cell numbers are similar in the hippocampal CA1-CA3 sectors and spinal cord to those in Tg(Thy1-MAPT*)30Schd mice; volumes of brain, cortex, hippocampal formation and cervical spinal cord are similar

abnormal brain morphology ( J:169074 )

• mice show significantly increased proportion of insoluble tau protein relative to sarkosyl-soluble tau compared to Tg(Thy1-MAPT*)30Schd single mutants

• soluble tau shows decreased phosphorylation compared to single transgenics, while insoluble tau displays significantly elevated phosporylation levels

• GSK-3 kinase activation in the brain is increased compared to wild-type and single mutants

neurofibrillary tangles ( J:169074 )

• NFTs are detected in the hippocampus, cortex, subcortical areas, brainstem, and spinal cord at 12 months, while no tangles are found in wild-type or Mapt mutants

• density (number) of NFTs is significantly increased in the hippocampus (subiculum and CA1 region) compared to Tg(Thy1-MAPT*)30Schd single mutants, but is similar between lines in the spinal cord

abnormal sciatic nerve morphology ( J:169074 )

• mice display axonal loss and reduction in cross-sectional area and axon density in the sciatic nerve compared to wild-type or Mapt mutants

abnormal spinal cord morphology ( J:169074 )

• mice develop numerous pericaryal rounded inclusions in the spinal cord; these are composed to abnormal filaments mixed with abundant neurofilaments; these are more numerous than seen in single transgenics

behavior/neurological

behavior/neurological phenotype ( J:169074 )

N	• in Y-maze tests, percentage of alternations observed is not significantly different from single transgenics, Mapt mutants or wild-type at 3-6 months of age

impaired coordination ( J:169074 )

• mice show a progressive motor deficit, significant from 6 to 12 months compared to wild-type or Mapt mutants; impairment is much more severe than in Tg(Thy1-MAPT*)30Schd single mutants

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

tau protein deposits ( J:84638 )

• phosphorylated tau accumulates in neuronal cell bodies and dendrites of the hippocampus and neocortex as early as 3 months of age

• in particular, accumulations occur in entorhinal cortex, ventromedial hypothalamus, medial septum and the nucleus of the horizontal limb of the diagonal band

• increase in tau phosphorylation occurs at serine 202, threonine 231 and serine 235 as determined by immunoblot

• tau aggregates in the proximal dendrites have an average width of 15 nm and are not densely packed

• insoluble tau is present in both 2 and 9 month old mice

• paired helical filaments are observed in 9, 12 and 14 month old mice

abnormal neuron morphology ( J:84638 )

• cells in the cortex and hippocampus appear irregularly shaped, often with distorted processes in 13 month old mice

hematopoietic system

abnormal spleen red pulp morphology ( J:174270 )

• splenic red pulp is largely obliterated

abnormal spleen B cell follicle morphology ( J:174270 )

• splenic white pulp contains follicles that are larger and more irregular in shape than normal follicles and are often fused with adjacent follicles

abnormal spleen B cell follicle shape ( J:174270 )

• irregular in shape

increased spleen B cell follicle size ( J:174270 )

immune system

abnormal spleen red pulp morphology ( J:174270 )

• splenic red pulp is largely obliterated

abnormal spleen B cell follicle morphology ( J:174270 )

• splenic white pulp contains follicles that are larger and more irregular in shape than normal follicles and are often fused with adjacent follicles

abnormal spleen B cell follicle shape ( J:174270 )

• irregular in shape

increased spleen B cell follicle size ( J:174270 )

renal/urinary system

abnormal renal glomerulus morphology ( J:174270 )

• kidney glomeruli are dilated probably due to obstruction from the amyloid in the lumen or walls of medullary tubules especially in the papilla

neoplasm

increased lymphoma incidence ( J:174270 )

• malignant lymphoma in the spleen and lymph nodes are seen in some mutants

• megakaryocytes are numerous is some spleens, suggesting a relation to megakaryocytic leukemia

increased follicular lymphoma incidence ( J:174270 )

• splenic architecture is replaced by proliferated immature mononuclear cells arranged in follicular aggregates indicating follicular lymphoma

homeostasis/metabolism

amyloidosis ( J:174270 )

• some mutants older than 18 months exhibit amyloid deposits in the spleen, kidneys, liver, ovary and/or heart

• amyloid is deposited concentrically in the perifollicular sheath, partly or completely encircling the follicles in the spleen

• in the liver, amyloid deposit is in the walls of sinusoids or central veins

• in the kidney, amyloid deposits are seen in the glomeruli

• amyloid deposits in the heart are not composed of amyloid-beta peptide, but rather amyloid A

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:84638 , J:174270

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/17/2024 MGI 6.24