About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tnfsf14tm1Kpf
targeted mutation 1, Klaus Pfeffer
MGI:2671122
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Tnfsf14tm1Kpf/Tnfsf14tm1Kpf B6.129P2-Tnfsf14tm1Kpf MGI:7277701
hm2
 		Tnfsf14tm1Kpf/Tnfsf14tm1Kpf involves: 129P2/OlaHsd MGI:5441223
hm3
 		Tnfsf14tm1Kpf/Tnfsf14tm1Kpf involves: 129P2/OlaHsd * C57BL/6 MGI:2671126
cx4
 		Cd28tm1Mak/Cd28tm1Mak
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf 		involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6 MGI:4441322
cx5
 		Rag1tm1Mom/Rag1tm1Mom
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf 		involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6 MGI:7277703
cx6
 		Ltbtm1Kpf/Ltbtm1Kpf
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf 		involves: 129P2/OlaHsd * C57BL/6 MGI:4441319
cx7
 		Tg(Tnfsf13b)1Fma/0
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf 		involves: 129P2/OlaHsd * C57BL/6 * DBA/2J MGI:3692946


Genotype
MGI:7277701
hm1
Allelic
Composition		 Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 B6.129P2-Tnfsf14tm1Kpf
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
abnormal osteoclast differentiation ( J:294030 )
• increase in osteoclast formation in ex vivo bone marrow cell cultures
abnormal thoracic vertebrae morphology ( J:294030 )
• end plates show disorganization of the chondrocyte columns
• chondrocyte columns are reduced in height in the hypertrophic zone and in the number of cells in the proliferative zone
• however, morphology of lumbar vertebrae do not appear abnormal
abnormal spine curvature ( J:294030 )
• deformity in the curvature of the spine at thoracic vertebrae in 40% of newborns
• abnormal curvature persists overtime
abnormal compact bone morphology ( J:294030 )
• significant decrease in bone formation parameters in cranial and femoral cortical and cancellous bone by dynamic histomorphometry analysis
abnormal trabecular bone morphology ( J:294030 )
• severely reduced structure in the femur at 1, 3, 6, and 12 months of age
• significant decrease in bone formation parameters in cranial and femoral cortical and cancellous bone by dynamic histomorphometry analysis
abnormal chondrocyte morphology ( J:294030 )
• end plates show disorganization of the chondrocyte columns in the thoracic vertebrae
• in thoracic vertebrae chondrocyte columns are reduced in height in the hypertrophic zone and in the number of cells in the proliferative zone

cellular
abnormal osteoclast differentiation ( J:294030 )
• increase in osteoclast formation in ex vivo bone marrow cell cultures

hematopoietic system
abnormal osteoclast differentiation ( J:294030 )
• increase in osteoclast formation in ex vivo bone marrow cell cultures

immune system
abnormal osteoclast differentiation ( J:294030 )
• increase in osteoclast formation in ex vivo bone marrow cell cultures




Genotype
MGI:5441223
hm2
Allelic
Composition		 Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal gamma-delta T cell morphology ( J:169863 )
• fewer IL17+ thymic gamma-delta T cells in response to PMA and ionomycin stimulation
• however, the number of thymic IFN-gamma+ gamma-delta T cells

immune system
abnormal gamma-delta T cell morphology ( J:169863 )
• fewer IL17+ thymic gamma-delta T cells in response to PMA and ionomycin stimulation
• however, the number of thymic IFN-gamma+ gamma-delta T cells




Genotype
MGI:2671126
hm3
Allelic
Composition		 Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:77250 )
• T cells show reduced proliferation after anti-CD3 antibody stimulation
• proliferation of CD4-depleted splenocytes after allogeneic MLR activation is reduced, while CD8-depleted splenocytes show no differences
decreased splenocyte proliferation ( J:77250 )
• splenocytes show reduced proliferation after anti-CD3 stimulation
abnormal T cell subpopulation ratio ( J:77250 )
• CD8/CD4 ratios of MLR-activated responder T cells are reduced by 40% on day 5 compared to wild-type splenocytes, however the ratio of naive splenocytes is similar to controls
decreased cytotoxic T cell cytolysis ( J:77250 )
• effector splenocytes show a reduction in specific target cell lysis compared to heterozygotes
decreased interferon-gamma secretion ( J:77250 )
• after allogeneic mixed lymphocyte reactive (MLR) activation
decreased interleukin-10 secretion ( J:77250 )
• after allogeneic MLR activation
decreased interleukin-2 secretion ( J:77250 )
• after allogeneic MLR activation
• production of IL-2 from CD8-depleted splenocytes after allogeneic MLR activation is reduced
decreased interleukin-4 secretion ( J:77250 )
• after allogeneic MLR activation

hematopoietic system
decreased T cell proliferation ( J:77250 )
• T cells show reduced proliferation after anti-CD3 antibody stimulation
• proliferation of CD4-depleted splenocytes after allogeneic MLR activation is reduced, while CD8-depleted splenocytes show no differences
decreased splenocyte proliferation ( J:77250 )
• splenocytes show reduced proliferation after anti-CD3 stimulation
abnormal T cell subpopulation ratio ( J:77250 )
• CD8/CD4 ratios of MLR-activated responder T cells are reduced by 40% on day 5 compared to wild-type splenocytes, however the ratio of naive splenocytes is similar to controls
decreased cytotoxic T cell cytolysis ( J:77250 )
• effector splenocytes show a reduction in specific target cell lysis compared to heterozygotes

cellular
decreased T cell proliferation ( J:77250 )
• T cells show reduced proliferation after anti-CD3 antibody stimulation
• proliferation of CD4-depleted splenocytes after allogeneic MLR activation is reduced, while CD8-depleted splenocytes show no differences
decreased splenocyte proliferation ( J:77250 )
• splenocytes show reduced proliferation after anti-CD3 stimulation




Genotype
MGI:4441322
cx4
Allelic
Composition		 Cd28tm1Mak/Cd28tm1Mak
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd28tm1Mak mutation (12 available); any Cd28 mutation (52 available)
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased length of allograft survival ( J:77250 )
• mutants show a longer skin graft survival of about 6 days than wild-type mice or single homozygotes




Genotype
MGI:7277703
cx5
Allelic
Composition		 Rag1tm1Mom/Rag1tm1Mom
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rag1tm1Mom mutation (49 available); any Rag1 mutation (123 available)
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
skeleton
abnormal trabecular bone morphology ( J:294030 )
• increase in cancellous bone structure in comparison to mice null for Rag alone

hematopoietic system

immune system




Genotype
MGI:4441319
cx6
Allelic
Composition		 Ltbtm1Kpf/Ltbtm1Kpf
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ltbtm1Kpf mutation (0 available); any Ltb mutation (19 available)
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal spleen morphology ( J:77250 )
• abnormal spleen architecture, with T cells accumulating around the central arteriole surrounded by a wall of B cells
abnormal mesenteric lymph node morphology ( J:77250 )
• only 4 out of 16 mutants have mesenteric lymph nodes
absent lymph nodes ( J:77250 )
• absent brachial, axillary, inguinal, popliteal, paraaortic, and parapancreatic lymph nodes

hematopoietic system
abnormal spleen morphology ( J:77250 )
• abnormal spleen architecture, with T cells accumulating around the central arteriole surrounded by a wall of B cells




Genotype
MGI:3692946
cx7
Allelic
Composition		 Tg(Tnfsf13b)1Fma/0
Tnfsf14tm1Kpf/Tnfsf14tm1Kpf
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6 * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Tnfsf13b)1Fma mutation (0 available)
Tnfsf14tm1Kpf mutation (2 available); any Tnfsf14 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
increased B cell number ( J:114782 )
• a marked increase in some B cell subsets compared to wild-type is detected in 12 week old mice
increased plasma cell number ( J:114782 )
• there is an increased frequency of B220neg/lo, IgA+ plasma cells in lamina propria preparations
abnormal spleen germinal center morphology ( J:114782 )
• numerous germinal centers are observed in B cell follicles of mutants
abnormal spleen marginal zone morphology ( J:114782 )
• splenic marginal zone is increased in size
increased IgA level ( J:114782 )
• augmented IgA staining is found in lamina propria of mutants

renal/urinary system
abnormal mesangial cell morphology ( J:114782 )
• in 8-month old mice, some thickening of mesangial and basement membranes
renal glomerular immunoglobulin deposits ( J:114782 )
• deposition of IgA in nearly all glomeruli is detected, similar to Tnfsf14-sufficient transgenic mice

immune system
increased B cell number ( J:114782 )
• a marked increase in some B cell subsets compared to wild-type is detected in 12 week old mice
increased plasma cell number ( J:114782 )
• there is an increased frequency of B220neg/lo, IgA+ plasma cells in lamina propria preparations
abnormal spleen germinal center morphology ( J:114782 )
• numerous germinal centers are observed in B cell follicles of mutants
abnormal spleen marginal zone morphology ( J:114782 )
• splenic marginal zone is increased in size
increased IgA level ( J:114782 )
• augmented IgA staining is found in lamina propria of mutants

cellular
abnormal mesangial cell morphology ( J:114782 )
• in 8-month old mice, some thickening of mesangial and basement membranes




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory