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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Mdm2tm1Mep
targeted mutation 1, Mary Ellen Perry
MGI:2673927
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Mdm2tm1Mep/Mdm2tm1Mep involves: 129S7/SvEvBrd * C57BL/6 MGI:3715450
ht2
 		Mdm2tm1Mep/Mdm2+ involves: 129S7/SvEvBrd * C57BL/6 MGI:3715451
ht3
 		Mdm2tm1Bay/Mdm2tm1Mep involves: 129S7/SvEvBrd * C57BL/6 MGI:3715443
ht4
 		Mdm2tm1Mep/Mdm2tm1.1Mep involves: 129S7/SvEvBrd * C57BL/6 * FVB MGI:3715446
cx5
 		Mdm2tm1Bay/Mdm2tm1Mep
Trp53tm1Tyj/Trp53tm1Tyj 		involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6 MGI:3715447


Genotype
MGI:3715450
hm1
Allelic
Composition		 Mdm2tm1Mep/Mdm2tm1Mep
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Mep mutation (1 available); any Mdm2 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
growth/size/body region phenotype ( J:81065 )
N
• body weight is comparable to wild-type




Genotype
MGI:3715451
ht2
Allelic
Composition		 Mdm2tm1Mep/Mdm2+
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Mep mutation (1 available); any Mdm2 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
increased mortality induced by gamma-irradiation ( J:81065 )
• 100% of mice die by day 20 after 10 Gy irradiation

homeostasis/metabolism
increased mortality induced by gamma-irradiation ( J:81065 )
• 100% of mice die by day 20 after 10 Gy irradiation




Genotype
MGI:3715443
ht3
Allelic
Composition		 Mdm2tm1Bay/Mdm2tm1Mep
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Bay mutation (1 available); any Mdm2 mutation (54 available)
Mdm2tm1Mep mutation (1 available); any Mdm2 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, incomplete penetrance ( J:81065 )
• number of offspring is less than the expected 25%; percentage of female offspring is 15-16%

growth/size/body
decreased body weight ( J:81065 )
• at 5 weeks of age, a 15-20% reduction in body weight is observed relative to wild-type mice

hematopoietic system
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
thymus hypoplasia ( J:81065 )
• decreased number of lymphoid cells; thymi are hypocellular
abnormal B cell differentiation ( J:81065 )
• 87% reduction in number of more mature B220+ IgM+ B cells
anemia ( J:81065 )
• at 5-6 weeks, mice exhibit mild anemia
decreased bone marrow cell number ( J:81065 )
• number of lymphoid cells is decreased 7-fold; mutants have only ~50% as many bone marrow cells as wild-type mice at 5-6 weeks
decreased erythrocyte cell number ( J:81065 )
• red blood cell count is 82% of wild-type; 2-fold decrease in number of erythrocytes is found at 5-6 weeks
decreased leukocyte cell number ( J:81065 )
• WBC count is only 30% of wild-type
decreased granulocyte number ( J:81065 )
• 1.2-fold decrease
decreased neutrophil cell number ( J:81065 )
• concentration of neutrophils is 60% of wild-type value
decreased lymphocyte cell number ( J:81065 )
• lymphocyte concentration is reduced to 37% of wild-type
decreased immature B cell number ( J:81065 )
• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
decreased T cell number ( J:81065 )
• reduced to 40% of wild-type number
decreased thymocyte number ( J:81065 )
• thymocyte number is 5% of wild-type
decreased double-negative T cell number ( J:81065 )
• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
decreased double-positive T cell number ( J:81065 )
• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
decreased spleen weight ( J:81065 )
• spleen wet weight is significantly less than in wild-type
spleen hypoplasia ( J:81065 )
• decreased number of lymphoid cells

immune system
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
thymus hypoplasia ( J:81065 )
• decreased number of lymphoid cells; thymi are hypocellular
abnormal B cell differentiation ( J:81065 )
• 87% reduction in number of more mature B220+ IgM+ B cells
decreased leukocyte cell number ( J:81065 )
• WBC count is only 30% of wild-type
decreased granulocyte number ( J:81065 )
• 1.2-fold decrease
decreased neutrophil cell number ( J:81065 )
• concentration of neutrophils is 60% of wild-type value
decreased lymphocyte cell number ( J:81065 )
• lymphocyte concentration is reduced to 37% of wild-type
decreased immature B cell number ( J:81065 )
• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
decreased T cell number ( J:81065 )
• reduced to 40% of wild-type number
decreased thymocyte number ( J:81065 )
• thymocyte number is 5% of wild-type
decreased double-negative T cell number ( J:81065 )
• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
decreased double-positive T cell number ( J:81065 )
• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
decreased spleen weight ( J:81065 )
• spleen wet weight is significantly less than in wild-type
spleen hypoplasia ( J:81065 )
• decreased number of lymphoid cells

liver/biliary system
decreased liver weight ( J:81065 )
• liver wet weight is less than in wild-type

renal/urinary system
decreased kidney weight ( J:81065 )
• kidney wet weight is less than in wild-type

cellular
abnormal cell cycle checkpoint function ( J:81065 )
• an increase in G1 arrest function is seen, with number of S-phase cells 72% of wild-type MEFs
increased cellular sensitivity to ionizing radiation ( J:81065 )
• exposure to 10 Gy of radiation results in 100% mortality within 7-9 days of treatment, compared to ~50% mortality in wild-type mice between 12 and 22 days after treatment
decreased cell proliferation ( J:81065 )
• MEFs in culture show decreased rate of proliferation compared to wild-type or Mdm2-heterozygous cells

endocrine/exocrine glands
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
thymus hypoplasia ( J:81065 )
• decreased number of lymphoid cells; thymi are hypocellular
decreased thymocyte number ( J:81065 )
• thymocyte number is 5% of wild-type




Genotype
MGI:3715446
ht4
Allelic
Composition		 Mdm2tm1Mep/Mdm2tm1.1Mep
Genetic
Background		 involves: 129S7/SvEvBrd * C57BL/6 * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1.1Mep mutation (1 available); any Mdm2 mutation (54 available)
Mdm2tm1Mep mutation (1 available); any Mdm2 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
decreased body weight ( J:81065 )
• at 5 weeks of age, a 15-20% reduction in body weight is observed relative to wild-type mice

hematopoietic system
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type
decreased spleen weight ( J:81065 )
• spleen wet weight is less than in wild-type

immune system
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type
decreased spleen weight ( J:81065 )
• spleen wet weight is less than in wild-type

liver/biliary system
decreased liver weight ( J:81065 )
• liver wet weight is significantly less than in wild-type

renal/urinary system
decreased kidney weight ( J:81065 )
• kidney wet weight is less than in wild-type

endocrine/exocrine glands
decreased thymus weight ( J:81065 )
• thymus wet weight is significantly less than in wild-type




Genotype
MGI:3715447
cx5
Allelic
Composition		 Mdm2tm1Bay/Mdm2tm1Mep
Trp53tm1Tyj/Trp53tm1Tyj
Genetic
Background		 involves: 129S2/SvPas * 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mdm2tm1Bay mutation (1 available); any Mdm2 mutation (54 available)
Mdm2tm1Mep mutation (1 available); any Mdm2 mutation (54 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (239 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:81065 )
N
• thymus is same size as in wild-type mice; thymocytes show wild-type levels of apoptosis and B cells show wild-type levels also
• number of B220+ B cells in bone marrow is similar to wild-type mice
abnormal bone marrow cell morphology/development ( J:81065 )
• 1.9-fold increase in number of apoptotice B cells relative to wild-type bone marrow

digestive/alimentary system
abnormal small intestine morphology ( J:81065 )
• 17-fold increase in spontaneous apoptosis, occurring mainly in the crypts rather than villi

mortality/aging
decreased mortality induced by ionizing radiation ( J:81065 )
• 100% of animals survive at least 35 days after treatment

homeostasis/metabolism
decreased mortality induced by ionizing radiation ( J:81065 )
• 100% of animals survive at least 35 days after treatment




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory