Phenotypes associated with this allele

• Allele Symbol: Rcan1^tm1Fmc
• Allele Name: targeted mutation 1, Frank McKeon
• Allele ID: MGI:2674908
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rcan1^tm1Fmc/Rcan1^tm1Fmc	involves: 129S4/SvJae	MGI:4359825
cx2	Rcan1^tm1Fmc/Rcan1^+ Ts(17^16)65Dn/0	involves: 129S4/SvJae * C3H/HeSn * C57BL/6 * C57BL/6Ei * DBA/2J	MGI:3850820

Genotype
MGI:4359825

hm1

• Allelic Composition: Rcan1^tm1Fmc/Rcan1^tm1Fmc
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Increased inflammatory leukocyte infiltration in sepsis in Rcan1^tm1Fmc/Rcan1^tm1Fmc heart and lung

immune system

abnormal T cell apoptosis ( J:85148 )

• T helper 1 cells undergo premature death during the proliferation phase of their activation

decreased T cell proliferation ( J:85148 )

• T cells exhibit a 50% reduction in proliferative response after activation with anti-CD3 and anti-CD28 antibodies

decreased IgG2a level ( J:85148 )

• mice immunized with 2,4,6-trinitrophenol-keyhole limpet hemocyanin (TNP-KLH) showed differences in immunoglobulin class switching, with a decrease in TNP-specific IgG2a concentration compared to wild-type mice

abnormal T-helper 1 physiology ( J:85148 )

• T helper 1 cells undergo premature death during the proliferation phase of their activation

decreased interferon-gamma secretion ( J:85148 )

• CD4+ T cells exhibit defective IFN-gamma production by 3 days of activation

• splenocytes from TNP-KLH immunized mice restimulated with KLH antigen produce less IFN-gamma than wild-type splenocytes

increased susceptibility to endotoxin shock ( J:152536 )

• endotoxemia in mutant mice leads to a higher reduction in heart rate, blood pressure, and body temperature and a greater increase in circulating IL-6 levels

• after LPS administration, leukocyte infiltration into the heart and lung is twice as great as controls

• all mice die within 84 hours of injection compared to 40% of wild-type controls

homeostasis/metabolism

abnormal circulating protein level ( J:152536 )

• VEGF levels in plasma are 5-fold higher than controls

• in response to LPS, VEGF levels are 2.2-fold higher than in LPS-injected wild-type mice

hematopoietic system

abnormal T cell apoptosis ( J:85148 )

• T helper 1 cells undergo premature death during the proliferation phase of their activation

decreased T cell proliferation ( J:85148 )

• T cells exhibit a 50% reduction in proliferative response after activation with anti-CD3 and anti-CD28 antibodies

decreased IgG2a level ( J:85148 )

• mice immunized with 2,4,6-trinitrophenol-keyhole limpet hemocyanin (TNP-KLH) showed differences in immunoglobulin class switching, with a decrease in TNP-specific IgG2a concentration compared to wild-type mice

abnormal T-helper 1 physiology ( J:85148 )

• T helper 1 cells undergo premature death during the proliferation phase of their activation

cellular

abnormal T cell apoptosis ( J:85148 )

• T helper 1 cells undergo premature death during the proliferation phase of their activation

decreased T cell proliferation ( J:85148 )

• T cells exhibit a 50% reduction in proliferative response after activation with anti-CD3 and anti-CD28 antibodies

Genotype
MGI:3850820

cx2

• Allelic Composition: Rcan1^tm1Fmc/Rcan1⁺; Ts(17¹⁶)65Dn/0
• Genetic Background: involves: 129S4/SvJae * C3H/HeSn * C57BL/6 * C57BL/6Ei * DBA/2J

neoplasm

decreased tumor growth/size ( J:150286 )

• transplanted Lewis lung and B16F10 tumor cells exhibit growth suppression with reduced microvessel density compared to tumor cells transplanted into wild-type mice but not as much as in Ts(17¹⁶)65Dn mice