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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Akt1tm1Hem
targeted mutation 1, Brian A Hemmings
MGI:2675202
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Akt1tm1Hem/Akt1tm1Hem involves: 129P2/OlaHsd MGI:3588771
hm2
 		Akt1tm1Hem/Akt1tm1Hem involves: 129P2/OlaHsd * C57BL/6 MGI:2675234
cx3
 		Akt1tm1Hem/Akt1+
Akt2tm1Hem/Akt2tm1Hem
Akt3tm1Hem/Akt3tm1Hem 		involves: 129P2/OlaHsd * C57BL/6 MGI:3691596
cx4
 		Akt1tm1Hem/Akt1tm1Hem
Akt2tm1Hem/Akt2tm1Hem
Akt3tm1Hem/Akt3tm1Hem 		involves: 129P2/OlaHsd * C57BL/6 MGI:3691595


Genotype
MGI:3588771
hm1
Allelic
Composition		 Akt1tm1Hem/Akt1tm1Hem
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Hem mutation (0 available); any Akt1 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
integument
thin hypodermis ( J:96826 )
• decreased hypodermis thickness
underdeveloped hair follicles ( J:96826 )
• follicular growth is delayed at P4, with skin displaying an increased proportion of underdeveloped hair follicles




Genotype
MGI:2675234
hm2
Allelic
Composition		 Akt1tm1Hem/Akt1tm1Hem
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Hem mutation (0 available); any Akt1 mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Structural abnormalities of E14 Akt1tm1Hem/Akt1tm1Hem mouse placenta

mortality/aging
postnatal lethality, incomplete penetrance ( J:85181 )
• about 40% of mutants are lost by 2-4 weeks after birth
• frequently, the majority of pups from homozygous intercross litters die within 10 days

embryo
abnormal placenta morphology ( J:85181 )
• placenta is not only small but also thin
abnormal placenta vasculature ( J:85181 )
• fetal placental vasculature is disordered with fewer, smaller and shorter vessels
• 52% decrease in total vessel length and 48% decrease in microvessel-covering area
• vascular branching is scarce
abnormal maternal decidual layer morphology ( J:85181 )
• the decidua is disproportionately decreased or missing in some mutant placentas
decreased spongiotrophoblast size ( J:85181 )
• the spongiotrophoblast of the placenta is smaller
decreased trophoblast glycogen cell number ( J:85181 )
• periodic acid Schiff's staining shows a reduction of glycogen-containing cells in the spongiotrophoblast
decreased placental labyrinth size ( J:85181 )
• the labyrinth of the placenta is smaller
small placenta ( J:85181 )
• severe hypotrophy of the placenta
decreased placenta weight ( J:85181 )
• placental weight is 24% lower than that of wild-type at E14.5
• while placenta weight increases from E14.5 to E16.5 in wild-type, in mutants, the weight of placenta does not change during this time period
abnormal placenta development ( J:85181 )
• placental hypotrophy with marked reduction of the decidual basalis and nearly complete loss of glycogen containing cells in the spongioblast
• decreased vascularization

growth/size/body
decreased body size ( J:85181 )
• mutants maintain small size from birth to adulthood
decreased body weight ( J:85181 )
• body weight of newborns is about 70% of wild-type littermates
postnatal growth retardation ( J:85181 )
• surviving mutants grow slowly after birth, with some extremely stunted and lean
decreased fetal size ( J:85181 )
• E14.5 fetuses are 20% smaller than wild-type

behavior/neurological
weakness ( J:85181 )
• newborns are weak

reproductive system
abnormal maternal decidual layer morphology ( J:85181 )
• the decidua is disproportionately decreased or missing in some mutant placentas

integument
pallor ( J:85181 )
• newborns are pale

cardiovascular system
abnormal placenta vasculature ( J:85181 )
• fetal placental vasculature is disordered with fewer, smaller and shorter vessels
• 52% decrease in total vessel length and 48% decrease in microvessel-covering area
• vascular branching is scarce




Genotype
MGI:3691596
cx3
Allelic
Composition		 Akt1tm1Hem/Akt1+
Akt2tm1Hem/Akt2tm1Hem
Akt3tm1Hem/Akt3tm1Hem
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Hem mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1Hem mutation (0 available); any Akt2 mutation (53 available)
Akt3tm1Hem mutation (0 available); any Akt3 mutation (42 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
growth/size/body
decreased body size ( J:114675 )
• viable and fertile and showed no obvious defects except for a severe growth deficiency
decreased body weight ( J:114675 )
• body weight at the age of 12 weeks reduced around 40% of wild-type




Genotype
MGI:3691595
cx4
Allelic
Composition		 Akt1tm1Hem/Akt1tm1Hem
Akt2tm1Hem/Akt2tm1Hem
Akt3tm1Hem/Akt3tm1Hem
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Akt1tm1Hem mutation (0 available); any Akt1 mutation (34 available)
Akt2tm1Hem mutation (0 available); any Akt2 mutation (53 available)
Akt3tm1Hem mutation (0 available); any Akt3 mutation (42 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
prenatal lethality, complete penetrance ( J:114675 )
• no triple homozygous mice were obtained from appropriate crosses implying embryonic lethality




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory