Phenotypes associated with this allele

• Allele Symbol: Ptpra^tm1Sap
• Allele Name: targeted mutation 1, Jan Sap
• Allele ID: MGI:2675252
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptpra^tm1Sap/Ptpra^tm1Sap	involves: 129S6/SvEvTac * 129X1/SvJ	MGI:2675253

Genotype
MGI:2675253

hm1

• Allelic Composition: Ptpra^tm1Sap/Ptpra^tm1Sap
• Genetic Background: involves: 129S6/SvEvTac * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal parental behavior ( J:55040 )

• homozygotes display nurturing abnormalities

growth/size/body

abnormal postnatal growth/weight/body size ( J:55040 )

• homozygotes survive to adulthood and are fertile, but exhibit gender-specific differences in body size

cellular

abnormal cell physiology ( J:55040 )

• in vitro, MEFs derived from homozygous mutant mice exhibit a 70% and 60% reduction in tyrosine kinase activity of c-Src and Fyn, respectively, as determined by measuring the amount of ³²P incorporated into an exogenous substrate (enolase), along with a concomitant increase in c-Src Tyr527 phosphorylation

• reduced c-Src kinase activity is also noted in tissue lysates from homozygous mutant mice

abnormal cell adhesion ( J:55040 )

• after initial attachment to a fibronectin-coated surface, mutant MEFs display an obvious but transient delay in integrin-mediated cell spreading, which is most prominent between 10 and 20 min after plating, similar to the spreading deficit reported for c-src gene inactivation

• in response to adhesion on a fibronectin substrate, mutant MEFs show impaired activation of integrin-induced signaling events, such as reduced tyrosine phosphorylation of the c-Src substrates p130^cas and Fak, and reduced activation of extracellular signal regulated (Erk) MAP kinases