Phenotypes associated with this allele

• Allele Symbol: Ccne2^tm1Pisc
• Allele Name: targeted mutation 1, Piotr Sicinski
• Allele ID: MGI:2675474
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ccne2^tm1Pisc/Ccne2^tm1Pisc	involves: 129S2/SvPas	MGI:2675496
cn2	Ccne1^tm3.1Pisc/Ccne1^tm3.1Pisc Ccne2^tm1Pisc/Ccne2^tm1Pisc	involves: 129S2/SvPas	MGI:5304329
cn3	Ccne1^tm3.1Pisc/Ccne1^tm3.1Pisc Ccne2^tm1Pisc/Ccne2^tm1Pisc Tg(Nes-cre)1Kln/0	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:5304330
cx4	Ccne1^tm1Pisc/Ccne1^tm1Pisc Ccne2^tm1Pisc/Ccne2^+	involves: 129S2/SvPas	MGI:2675490
cx5	Ccne1^tm1Pisc/Ccne1^+ Ccne2^tm1Pisc/Ccne2^tm1Pisc	involves: 129S2/SvPas	MGI:2675491
cx6	Ccne1^tm1Pisc/Ccne1^tm1Pisc Ccne2^tm1Pisc/Ccne2^tm1Pisc	involves: 129S2/SvPas	MGI:2675493
cx7	Ccne1^tm2.1Pisc/Ccne1^tm2.1Pisc Ccne2^tm1Pisc/Ccne2^tm1Pisc	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:5304328

Genotype
MGI:2675496

hm1

• Allelic Composition: Ccne2^tm1Pisc/Ccne2^tm1Pisc
• Genetic Background: involves: 129S2/SvPas

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal reproductive system morphology ( J:85224 )

multinucleated giant male germ cells ( J:85224 )

♂

abnormal seminiferous tubule morphology ( J:85224 )

♂ • thin walls

♂ • greatly reduced number of spermatogenic cells

♂ • multinucleate giant cells in epithelium

small testis ( J:85224 )

♂

testicular atrophy ( J:85224 )

♂

abnormal spermatogenesis ( J:85224 )

♂

oligozoospermia ( J:85224 )

♂

abnormal male meiosis ( J:85224 )

♂ • abnormal meiotic figures observed in spermatocyte layers

male infertility ( J:85224 )

♂ • 50% of males are sterile

♂ • females are fully fertile

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:85224 )

♂ • thin walls

♂ • greatly reduced number of spermatogenic cells

♂ • multinucleate giant cells in epithelium

small testis ( J:85224 )

♂

testicular atrophy ( J:85224 )

♂

cellular

oligozoospermia ( J:85224 )

♂

multinucleated giant male germ cells ( J:85224 )

♂

abnormal male meiosis ( J:85224 )

♂ • abnormal meiotic figures observed in spermatocyte layers

Genotype
MGI:5304329

cn2

• Allelic Composition: Ccne1^tm3.1Pisc/Ccne1^tm3.1Pisc; Ccne2^tm1Pisc/Ccne2^tm1Pisc
• Genetic Background: involves: 129S2/SvPas

nervous system

abnormal dendritic spine morphology ( J:178882 )

• hippocampal slices transfected with cre exhibit decreased dendritic spine numbers compared with control neurons

Genotype
MGI:5304330

cn3

• Allelic Composition: Ccne1^tm3.1Pisc/Ccne1^tm3.1Pisc; Ccne2^tm1Pisc/Ccne2^tm1Pisc; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

nervous system

nervous system phenotype ( J:178882 )

N • mice exhibit normal proliferation rates throughout embryonic brain development, numbers of proliferating progenitor cells in the subventricular zone, and numbers of neural stem/progenitor cells

abnormal dendritic spine morphology ( J:178882 )

• adult mice exhibit reduced spine length, width, and estimated spine volume compared with control mice

abnormal postsynaptic density morphology ( J:178882 )

• in the CA1 stratum radiatum, neurons exhibit reduced length of postsynaptic densities compared with control neurons

decreased excitatory postsynaptic current frequency ( J:178882 )

• spontaneous excitatory postsynaptic current frequency in CA1 pyramidal neurons is reduced compared to in control neurons

abnormal excitatory postsynaptic potential ( J:178882 )

• field excitatory postsynaptic potential amplitude and slope are decreased compared to in control mice

reduced NMDA-mediated synaptic currents ( J:178882 )

reduced long-term potentiation ( J:178882 )

behavior/neurological

abnormal spatial learning ( J:178882 )

• mice exhibit impaired performance in a Morris water maze and spatial reversal learning compared with control mice

Genotype
MGI:2675490

cx4

• Allelic Composition: Ccne1^tm1Pisc/Ccne1^tm1Pisc; Ccne2^tm1Pisc/Ccne2⁺
• Genetic Background: involves: 129S2/SvPas

normal phenotype

no abnormal phenotype detected ( J:85224 )

Genotype
MGI:2675491

cx5

• Allelic Composition: Ccne1^tm1Pisc/Ccne1⁺; Ccne2^tm1Pisc/Ccne2^tm1Pisc
• Genetic Background: involves: 129S2/SvPas

endocrine/exocrine glands

testis hypoplasia ( J:85224 )

♂

reproductive system

abnormal reproductive system morphology ( J:85224 )

• phenotype like that of homozygous Ccne2^tm1Pisc but more severe

testis hypoplasia ( J:85224 )

♂

abnormal fertility/fecundity ( J:85224 )

• phenotype like that of homozygous Ccne2^tm1Pisc but more severe

reduced male fertility ( J:85224 )

♂

Genotype
MGI:2675493

cx6

• Allelic Composition: Ccne1^tm1Pisc/Ccne1^tm1Pisc; Ccne2^tm1Pisc/Ccne2^tm1Pisc
• Genetic Background: involves: 129S2/SvPas

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:85224 )

• expected number of homozygotes through E10.25

• only 50% of expected number of homozygotes found at E10.75

• no live double homozygotes after E11.5

cardiovascular system

abnormal cardiovascular system morphology ( J:85224 )

• observed in embryos rescued by tetraploid complementation

abnormal aortic arch development ( J:85224 )

• abnormal patterning of the aortic arch, possibly resulting from a defect in neural crest migration

double outlet right ventricle ( J:85224 )

ostium primum atrial septal defect ( J:85224 )

• observed in embryos rescued by tetraploid complementation

atrioventricular septal defect ( J:85224 )

ventricular septal defect ( J:85224 )

• observed in embryos rescued by tetraploid complementation

embryo

decreased embryo size ( J:85224 )

• normal morphogenesis and no pathological lesions despite growth retardation at E10.75

abnormal extraembryonic tissue morphology ( J:85224 )

abnormal placenta morphology ( J:85224 )

• partial rescue of embryonic lethality by tetraploid complementation proves that a problem exists in the placenta

abnormal trophoblast giant cell morphology ( J:85224 )

• layer of trophoblast giant cells reduced or absent

• greatly reduced DNA content relative to expectation

growth/size/body

decreased embryo size ( J:85224 )

• normal morphogenesis and no pathological lesions despite growth retardation at E10.75

hematopoietic system

abnormal definitive hematopoiesis ( J:85224 )

• observed in embryos rescued by tetraploid complementation

abnormal thrombopoiesis ( J:85224 )

• reduced DNA content in megakaryocytes relative to expectation observed in embryos rescued by tetraploid complementation

immune system

abnormal thrombopoiesis ( J:85224 )

• reduced DNA content in megakaryocytes relative to expectation observed in embryos rescued by tetraploid complementation

Genotype
MGI:5304328

cx7

• Allelic Composition: Ccne1^tm2.1Pisc/Ccne1^tm2.1Pisc; Ccne2^tm1Pisc/Ccne2^tm1Pisc
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:178882 )

• mice are viable, fertile, and phenotypically normal