Phenotypes associated with this allele

• Allele Symbol: Grb2^tm2Paw
• Allele Name: targeted mutation 2, Tony Pawson
• Allele ID: MGI:2677457
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Grb2^tm2Paw/Grb2^tm2Paw	Not Specified	MGI:3719994
ht2	Grb2^tm1Paw/Grb2^tm2Paw	involves: 129S1/Sv * 129X1/SvJ	MGI:3719995

Genotype
MGI:3719994

hm1

• Allelic Composition: Grb2^tm2Paw/Grb2^tm2Paw
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:69138 )

• although mice develop to term none survive to P1

embryo

decreased placental labyrinth size ( J:69138 )

• placental labyrinth is reduced

craniofacial

abnormal mandible morphology ( J:69138 )

• in 3 of 22 mice

palatal shelves fail to meet at midline ( J:69138 )

• in newborns, palatal shelves remain cleft

cleft secondary palate ( J:69138 )

skeleton

abnormal mandible morphology ( J:69138 )

• in 3 of 22 mice

cardiovascular system

hemorrhage ( J:69138 )

• at E13.5 to E14.5, 6 of 22 mice are hemorrhagic or edemic

growth/size/body

palatal shelves fail to meet at midline ( J:69138 )

• in newborns, palatal shelves remain cleft

cleft secondary palate ( J:69138 )

decreased body length ( J:69138 )

• mice are smaller (2.43+/-0.15 cm) compared to wild-type mice (2.80+/-0.12 cm)

homeostasis/metabolism

edema ( J:69138 )

• at E13.5 to E14.5, 6 of 22 mice are hemorrhagic or edemic

digestive/alimentary system

palatal shelves fail to meet at midline ( J:69138 )

• in newborns, palatal shelves remain cleft

cleft secondary palate ( J:69138 )

behavior/neurological

absent gastric milk in neonates ( J:69138 )

Genotype
MGI:3719995

ht2

• Allelic Composition: Grb2^tm1Paw/Grb2^tm2Paw
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:69138 )

• by E11.5 mice are no longer viable

embryo

absent second pharyngeal arch ( J:69138 )

• at E9 to E10

decreased placental labyrinth size ( J:69138 )

• placental labyrinth is reduced

failure of chorioallantoic fusion ( J:69138 )

• at E9.5 to E10.5, chorioallantoic fusion fails to occur in 32% of mice

cardiovascular system

abnormal heart development ( J:69138 )

hearing/vestibular/ear

abnormal otic vesicle development ( J:69138 )

• large clusters of pycnotic nuclei are present in greater numbers (n=15) in the otic vesicle than in wild-type mice (n=11)

• mesenchyme cells in the otic vesicle undergo more apoptosis than in wild-type mice

nervous system

abnormal facial nerve morphology ( J:69138 )

• cranial nerve VII wanders at the dorsal body edge

abnormal vagus nerve morphology ( J:69138 )

• disorganized

craniofacial

absent second pharyngeal arch ( J:69138 )

• at E9 to E10