Analysis Tools
mortality/aging
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• less than 1.7% of homozygotes are detected at weaning, indicating that a very low percentage of homozygotes develop through E7.5 and survive to term with no obvious defects
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• most mutant embryos are almost completely resorbed by E8.5
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embryo
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• only ~1.7% of mutant embryos develop beyond E7.5
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• at E6.5-E7, mutant embryos are at least 5 times smaller than wild-type embryos
• by E7.5, mutant embryos are at least 10 times smaller than wild-type embryos
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• at E7.5, mutant embryos display absence of mesoderm
• in situ hybridization and RT-PCR analyses of affected E7.5 embryos indicate ectopic myosin light chain transcripts, whose products are predicted to interfere with various nonmuscle cell functions determining differentiation of mesoderm
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• at E7.5, mutant embryos lack a primitive streak
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• at E6.5-E7, a large portion of the yolk sac cavity remains empty, suggesting that the egg cylinder fails to properly fill the cavity
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• at E6.5, mutant embryos display abnormal double-sided vacuoles in cells of the extraembryonic endoderm
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growth/size/body
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• at E6.5-E7, mutant embryos are at least 5 times smaller than wild-type embryos
• by E7.5, mutant embryos are at least 10 times smaller than wild-type embryos
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