Phenotypes associated with this allele

• Allele Symbol: Crhr1^tm2Wrst
• Allele Name: targeted mutation 2, Wolfgang Wurst
• Allele ID: MGI:2678096
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Crhr1^tm2Wrst/Crhr1^tm2Wrst	involves: 129S2/SvPas * C57BL/6	MGI:2678098
cn2	Crhr1^tm2Wrst/Crhr1^tm2Wrst Neurod6^tm1(cre)Kan/Neurod6^+	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6J	MGI:5294458
cn3	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(Camk2a-cre)159Kln/0	involves: 129S2/SvPas * C57BL/6	MGI:2678102
cn4	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(Nes-cre)1Kln/0	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * SJL	MGI:5294457
cn5	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(Fev-cre)1Esd/0	involves: 129S2/SvPas * C57BL/6 * SJL	MGI:5294460
cn6	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(Slc6a3-cre/ERT2)1Span/0	involves: 129S2/SvPas * C57BL/6J	MGI:5294461
cn7	Crhr1^tm2Wrst/Crhr1^tm2Wrst Tg(dlx5a-cre)1Mekk/0	involves: 129S2/SvPas * C57BL/6J * CD-1	MGI:5294459

Genotype
MGI:2678098

hm1

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:85745 )

• homozygotes do not display any behavioral or neuroendocrine phenotype

Genotype
MGI:5294458

cn2

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Neurod6^tm1(cre)Kan/Neurod6⁺
• Genetic Background: involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • mice exhibit normal behavior in a forced swim test and auditory fear conditioning

decreased anxiety-related response ( J:176339 )

• in a dark-light box, elevated plus maze, and novel object exploration tests

hyperactivity ( J:176339 )

nervous system

abnormal CNS synaptic transmission ( J:176339 )

• mice exhibit increased corticosterone-releasing hormone (CRH)-evoked excitatory field potentials on glutamatergic neurons compared with control mice

• CRH fails to enhance propagation of neuronal activity from hippocampal input region and CA1 input compared with control mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:176339 )

N • mice exhibit normal basal and stress-induced corticosterone levels

Genotype
MGI:2678102

cn3

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(Camk2a-cre)159Kln/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6

behavior/neurological

decreased anxiety-related response ( J:85745 )

• in the light-dark box paradigm, mutant mice spend significantly more time in the lit compartment, display a decresead latency to enter the lit compartment, and show a signicantly higher number of entries into the lit compartment than control mice; however, no significant differences in the number of entries into the dark compartment are observed

• in the elevated plus maze test, mutant mice display significantly fewer head-dips in the protected arms as a percentage of total head dips, and an increase in the percentage of entries into the open arms; however, no significant differences in the number of entries into the closed arms are observed

increased locomotor activity ( J:85745 )

• mutant mice display increased locomotor activity in the open-field paradigm

homeostasis/metabolism

increased circulating corticosterone level ( J:85745 )

• although basal plasma corticosterone levels are normal, mutant mice display significantly higher plasma corticosterone levels at 30 and 90 min after a 5-min restraint stress relative to similarly-stressed control mice

increased circulating adrenocorticotropin level ( J:85745 )

• although basal plasma ACTH levels are normal, mutant mice display significantly higher plasma ACTH levels at 30 and 90 min after a 5-min restraint stress relative to similarly-stressed control mice

Genotype
MGI:5294457

cn4

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * C57BL/6J * SJL

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • mice exhibit normal anxiety-related behavior

Genotype
MGI:5294460

cn5

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(Fev-cre)1Esd/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * SJL

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • mice exhibit normal behavior in a forced swim test and auditory fear conditioning

increased anxiety-related response ( J:176339 )

• in a dark-light box, elevated plus maze, the novel object exploration, and modified hole board tests

• hypoactivity in the first 5 minutes of the open field test

decreased locomotor activity ( J:176339 )

• in the first 5 minutes of the open field test

nervous system

abnormal synaptic dopamine release ( J:176339 )

• mice exhibit decreased stress-induced dopamine release in the pre-frontal cortex compared with control mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:176339 )

N • mice exhibit normal basal and stress-induced corticosterone levels

Genotype
MGI:5294461

cn6

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(Slc6a3-cre/ERT2)1Span/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • tamoxifen-treated mice exhibit normal anxiety-related behavior, behavior in a forced swim test, and auditory fear conditioning

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:176339 )

N • tamoxifen-treated mice exhibit normal basal and stress-induced corticosterone levels

Genotype
MGI:5294459

cn7

• Allelic Composition: Crhr1^tm2Wrst/Crhr1^tm2Wrst; Tg(dlx5a-cre)1Mekk/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J * CD-1

behavior/neurological

behavior/neurological phenotype ( J:176339 )

N • mice exhibit normal anxiety-related behavior, forced swim test, and auditory fear conditioning

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:176339 )

N • mice exhibit normal basal and stress-induced corticosterone levels