Phenotypes associated with this allele

• Allele Symbol: Ikbkb^tm1.1Cgn
• Allele Name: targeted mutation 1.1, University of Cologne
• Allele ID: MGI:2678816
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ikbkb^tm1.1Cgn/Ikbkb^tm1.1Cgn	involves: C57BL/6	MGI:2679015
cn2	Cd19^tm1(cre)Cgn/Cd19^+ Ikbkb^tm1Cgn/Ikbkb^tm1.1Cgn	involves: 129P2/OlaHsd * C57BL/6	MGI:3851693
cn3	Ikbkb^tm1Cgn/Ikbkb^tm1.1Cgn Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * DBA/2	MGI:2679019

Genotype
MGI:2679015

hm1

• Allelic Composition: Ikbkb^tm1.1Cgn/Ikbkb^tm1.1Cgn
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:85810 )

• die during embryogenesis

immune system

decreased interleukin-6 secretion ( J:79132 , J:85810 )

• TNF-mediated IL-6 production is reduced but not abolished in these embryonic cells (J:79132)

• production of IL-6 in response to IL-1, LPS, or TNF is reduced in MEFs compared to IKbkb^tm2.1Cgn MEFs (J:85810)

cellular

increased fibroblast apoptosis ( J:85810 )

• mouse embryonic fibroblasts (MEFs) are more sensitive to TNF-induced apoptosis than IKbkb^tm2.1Cgn MEFs

Genotype
MGI:3851693

cn2

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Ikbkb^tm1Cgn/Ikbkb^tm1.1Cgn
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

increased B cell proliferation ( J:79132 )

• B cell turnover over a one week period is almost twice that of controls

decreased immature B cell number ( J:79132 )

• immature B cell numbers in the spleen are reduced by about a half

decreased mature B cell number ( J:79132 )

• there is a 2- to 4- fold reduction in the number of mature recirculating B cells found in the bone marrow

• the IgM^lowIgD⁺ mature B cell population is strongly diminished in the spleen by 3- to 4- fold

• B cell numbers in the lymph nodes are also strongly reduced

decreased B-1 B cell number ( J:79132 )

• B-1 B cell numbers are strongly decreased in the peritoneum cavity

decreased follicular B cell number ( J:79132 )

• follicular B cells are the most diminished B cell population in the spleen

decreased marginal zone B cell number ( J:79132 )

• marginal zone B cell numbers are reduced about 4-fold

hematopoietic system

increased B cell proliferation ( J:79132 )

• B cell turnover over a one week period is almost twice that of controls

decreased immature B cell number ( J:79132 )

• immature B cell numbers in the spleen are reduced by about a half

decreased mature B cell number ( J:79132 )

• there is a 2- to 4- fold reduction in the number of mature recirculating B cells found in the bone marrow

• the IgM^lowIgD⁺ mature B cell population is strongly diminished in the spleen by 3- to 4- fold

• B cell numbers in the lymph nodes are also strongly reduced

decreased B-1 B cell number ( J:79132 )

• B-1 B cell numbers are strongly decreased in the peritoneum cavity

decreased follicular B cell number ( J:79132 )

• follicular B cells are the most diminished B cell population in the spleen

decreased marginal zone B cell number ( J:79132 )

• marginal zone B cell numbers are reduced about 4-fold

cellular

increased B cell proliferation ( J:79132 )

• B cell turnover over a one week period is almost twice that of controls

Genotype
MGI:2679019

cn3

• Allelic Composition: Ikbkb^tm1Cgn/Ikbkb^tm1.1Cgn; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

decreased T cell number ( J:85810 )

• spleen and lymph nodes are nearly devoid of T cells

decreased CD4-positive, alpha-beta T cell number ( J:85810 )

• only a mild reduction in the CD4 single-positive cells in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:85810 )

• 50% reduction in CD8 single-positive cells in the thymus

decreased single-positive T cell number ( J:85810 )

• loss of single positive thymocytes during final maturation stages in the thymus

hematopoietic system

decreased T cell number ( J:85810 )

• spleen and lymph nodes are nearly devoid of T cells

decreased CD4-positive, alpha-beta T cell number ( J:85810 )

• only a mild reduction in the CD4 single-positive cells in the thymus

decreased CD8-positive, alpha-beta T cell number ( J:85810 )

• 50% reduction in CD8 single-positive cells in the thymus

decreased single-positive T cell number ( J:85810 )

• loss of single positive thymocytes during final maturation stages in the thymus