Phenotypes associated with this allele

• Allele Symbol: Caml^tm1Rjb
• Allele Name: targeted mutation 1, Richard J Bram
• Allele ID: MGI:2678819
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Caml^tm1Rjb/Caml^tm1Rjb	involves: 129X1/SvJ	MGI:2678820
cn2	Caml^tm1Rjb/Caml^tm2Rjb Tg(Lck-cre)1Cwi/0	involves: 129 * C57BL/6 * DBA/2	MGI:3590220
cn3	Caml^tm1Rjb/Caml^tm2Rjb Tg(Lck-cre)1Cwi/0 Tg(TcraTcrb)1100Mjb/0	involves: 129 * C57BL/6 * DBA/2	MGI:3590221

Genotype
MGI:2678820

hm1

• Allelic Composition: Caml^tm1Rjb/Caml^tm1Rjb
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:85708 )

• death during early gestation, prior to E7

Genotype
MGI:3590220

cn2

• Allelic Composition: Caml^tm1Rjb/Caml^tm2Rjb; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

hematopoietic system

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

decreased single-positive T cell number ( J:100536 )

• 4- to 6-fold fewer CD4 and CD8 single-positive cells compared to control

• in 3-4 weeks old mutant mice, there were 7- to 9-fold fewer peripheral CD4+ and CD8+ T cells

• in 8-9 weeks old mutant mice, T cell numbers were more similar

immune system

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

decreased single-positive T cell number ( J:100536 )

• 4- to 6-fold fewer CD4 and CD8 single-positive cells compared to control

• in 3-4 weeks old mutant mice, there were 7- to 9-fold fewer peripheral CD4+ and CD8+ T cells

• in 8-9 weeks old mutant mice, T cell numbers were more similar

cellular

increased T cell apoptosis ( J:100536 )

• double-positive thymocytes from mutant mice undergo enhanced apoptosis by TCR-stimulation

endocrine/exocrine glands

decreased thymocyte number ( J:100536 )

• the average numbers of total thymocytes were reduced by half

• the number of cells at the double-negative stage was not different

Genotype
MGI:3590221

cn3

• Allelic Composition: Caml^tm1Rjb/Caml^tm2Rjb; Tg(Lck-cre)1Cwi/0; Tg(TcraTcrb)1100Mjb/0
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

hematopoietic system

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes

abnormal negative T cell selection ( J:100536 )

• in an in vitro assay, mutant thymocytes underwent OCA peptide 257-264 dependent cell death more readily than control, indicating enhanced negative selection

abnormal positive T cell selection ( J:100536 )

• lower proportions of double-positive and single-positive cells, and reduced CD8 SP cell in the peripheral T cell population, both indicating impaired positive selection

immune system

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes

abnormal negative T cell selection ( J:100536 )

• in an in vitro assay, mutant thymocytes underwent OCA peptide 257-264 dependent cell death more readily than control, indicating enhanced negative selection

abnormal positive T cell selection ( J:100536 )

• lower proportions of double-positive and single-positive cells, and reduced CD8 SP cell in the peripheral T cell population, both indicating impaired positive selection

endocrine/exocrine glands

decreased thymocyte number ( J:100536 )

• reduced numbers of total thymocytes