Phenotypes associated with this allele

• Allele Symbol: Kcnip3^tm1Bux
• Allele Name: targeted mutation 1, Joseph D Buxbaum
• Allele ID: MGI:2679303
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnip3^tm1Bux/Kcnip3^tm1Bux	involves: 129S4/SvJae * C57BL/6	MGI:2679304

Genotype
MGI:2679304

hm1

• Allelic Composition: Kcnip3^tm1Bux/Kcnip3^tm1Bux
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:85927 )

N • at 6-8 months of age, homozygotes display normal motor coordination, balance, and fine motor control of the forelimbs relative to wild-type littermates

abnormal spatial learning ( J:85927 )

• in the Morris water maze test, homozygotes exhibit shorter escape latencies than wild-type littermates

abnormal response to tactile stimuli ( J:85927 )

• homozygotes exhibit a prolonged latency in the tail-flick assay relative to wild-type littermates, suggesting hyporesponsiveness to tactile stimuli

• in contrast, the % of maximum electrical shock level (0.08 mA) required to elicit running and vocalization is significantly lower in mutant mice, indicating an increase in shock sensitivity relative to wild-type mice

hyperactivity ( J:85927 )

• homozygotes exhibit an increased number of movements relative to wild-type littermates, as detected with infrared monitors during a 30 min session

nervous system

enhanced long-term potentiation ( J:85927 )

• homozygotes display enhanced LTP in the dentate gyrus of the hippocampus, as shown by an increase in the average potentiation of the fEPSP slope at 60 min after tetanic stimulation

• notably, LTP in CA1 pyramidal cells (induced by tetanic stimulation of Schaffer collaterals) appears unchanged, and basic synaptic transmission and paired pulse facilitation remain relatively normal

• enhanced LTP coincides with a decrease in the transient Kv4 channel-dependent A-type current and can be mimicked in wild-type mice by phrixotoxin-2 (a Kv4 channel blocker)

abnormal channel response ( J:85927 )

• homozygotes display a significant decrease in the Kv4 channel-dependent transient A-type current from dentate gyrus granule cells

homeostasis/metabolism

abnormal protein level ( J:85927 )

• in the cerebellum, homozygotes exhibit a ~50% reduction in the levels of both Abeta40 and Abeta42 peptides relative to wild-type littermates, indicating an effect on presenilin-dependent gamma-secretase cleavage in vivo

• in the cortex, Abeta40 peptide levels are reduced to a lesser extent while Abeta42 levels remain unaffected

• Abeta peptide levels display a trend toward reduction in the hippocampus

• however, changes in Abeta levels are not associated with any detectable changes in amyloid protein precursor (APP) processing