Phenotypes associated with this allele

• Allele Symbol: Cplx2^tm1Bros
• Allele Name: targeted mutation 1, Nils Brose
• Allele ID: MGI:2679626
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cplx2^tm1Bros/Cplx2^tm1Bros	B6NCrl.Cg-Cplx2^tm1Bros	MGI:5285873
hm2	Cplx2^tm1Bros/Cplx2^tm1Bros	Not Specified	MGI:2679652
cx3	Cplx1^tm2Bros/Cplx1^tm2Bros Cplx2^tm1Bros/Cplx2^tm1Bros	Not Specified	MGI:2679648
cx4	Cplx1^tm2Bros/Cplx1^tm2Bros Cplx2^tm1Bros/Cplx2^tm1Bros Cplx3^tm1Bros/Cplx3^tm1Bros	Not Specified	MGI:3807187
cx5	Cplx2^tm1Bros/Cplx2^tm1Bros Cplx3^tm1Bros/Cplx3^tm1Bros	Not Specified	MGI:3807188

Genotype
MGI:5285873

hm1

• Allelic Composition: Cplx2^tm1Bros/Cplx2^tm1Bros
• Genetic Background: B6NCrl.Cg-Cplx2^tm1Bros

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:175056 )

N • independent of parietal cortical neurotrauma, mice exhibit normal social interaction, sucrose preference test for anhedonia, forced alternation in a T-maze, novel object recognition, and pre-pulse inhibition

abnormal spatial learning ( J:175056 )

• in a Morris water maze, independent of parietal cortical neurotrauma

abnormal spatial reference memory ( J:175056 )

• in a Morris water maze, mice subjected to parietal cortical neurotrauma exhibit increased latency to escape by trial 6 through 8 compared with similarly treated wild-type mice

increased thigmotaxis ( J:175056 )

• independent of parietal cortical neurotrauma, mice spend less time in the center and intermediate area with increased time in the periphery of an open field compared with similarly treated wild-type mice

• however, mice exhibit normal anxiety-related behaviors in an elevated plus maze

increased startle reflex ( J:175056 )

• following parietal cortical neurotrauma

limb grasping ( J:175056 )

• in mice subjected to parietal cortical neurotrauma

impaired coordination ( J:175056 )

• on a rotarod, independent of parietal cortical neurotrauma

decreased locomotor activity ( J:175056 )

• in an open field, independent of parietal cortical neurotrauma

induced hyperactivity ( J:175056 )

• MK-801-treated mice subjected to parietal cortical neurotrauma exhibit increased hyperlocomotion compared with similarly treated wild-type mice

nervous system

decreased hippocampus volume ( J:175056 )

• reduced volume following parietal cortical neurotrauma

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
schizophrenia		DOID:5419	OMIM:181500	J:175056

Genotype
MGI:2679652

hm2

• Allelic Composition: Cplx2^tm1Bros/Cplx2^tm1Bros
• Genetic Background: Not Specified

behavior/neurological

abnormal learning/memory/conditioning ( J:85961 )

decreased exploration in new environment ( J:85961 )

• reduced exploration in new environment

• less active in open field

• initial higher latency to reach periphery but normal after the first day

abnormal spatial learning ( J:85961 )

• slower at 8 weeks in the Morris water maze but successful in acquisition and learning new positions, much slower at 1 year

• generally poor in acquisition learning and very poor in reversal learning

abnormal fear/anxiety-related behavior ( J:85961 )

abnormal anxiety-related response ( J:85961 )

• impaired visual cliff avoidance test, more likely to approach the "cliff" and spend more time there

abnormal grooming behavior ( J:85961 )

abnormal whisker trimming behavior ( J:85961 )

• keep whiskers for longer period of time

decreased grooming behavior ( J:85961 )

abnormal motor capabilities/coordination/movement ( J:85961 )

impaired coordination ( J:85961 )

• rotarod performance decreases with time, initially normal, poor at 24 weeks

abnormal posture ( J:85961 )

• pelvis held lower than normal

tail dragging ( J:85961 )

abnormal locomotor behavior ( J:85961 )

novel environmental response-related retropulsion ( J:85961 )

• one third of mice move backward rather than forward when placed in a new environment

abnormal gait ( J:85961 )

• tend not to walk in a straight line, follow a weaving path

short stride length ( J:85961 )

decreased vertical activity ( J:85961 )

• less rearing activity

• fewer attempts to climb out of cages although strength is normal by hang wire test

Genotype
MGI:2679648

cx3

• Allelic Composition: Cplx1^tm2Bros/Cplx1^tm2Bros; Cplx2^tm1Bros/Cplx2^tm1Bros
• Genetic Background: Not Specified

mortality/aging

neonatal lethality, complete penetrance ( J:86365 , J:136393 )

• die within a few hours of birth (J:86365)

• mice die within a few hours of birth (J:136393)

nervous system

abnormal pre-Botzinger complex physiology ( J:136393 )

• the amplitude of spontaneous excitatory postsynaptic currents are decreased in pre-Botzinger complex neurons compared to in wild-type mice

abnormal neuron physiology ( J:136393 )

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

impaired synaptic plasticity ( J:136393 )

• trains of action potentials produce a marked initial facilitation compared to the depression observed in wild-type mice

abnormal CNS synaptic transmission ( J:86365 )

• much less of neurotransmitter released

• defect coupling action potential to presynaptic release

• hippocampal EPSC amplitudes reduced 66% but increases with trains of stimuli

• affect is on fast synchronous release

• decreased Ca+2 sensitivity

decreased excitatory postsynaptic current amplitude ( J:136393 )

• evoked and spontaneous excitatory postsynaptic current amplitudes in hippocampal neurons are reduced compared to in wild-type mice

• the amplitude of spontaneous excitatory postsynaptic currents are decreased in pre-Botzinger complex neurons compared to in wild-type mice

abnormal inhibitory postsynaptic currents ( J:136393 )

• mice exhibit a reduced evoked inhibitory postsynaptic currents and slightly reduced miniature inhibitory postsynaptic currents compared to wild-type mice

• spontaneous miniature inhibitory postsynaptic current frequencies are decreased in hippocampal and pre-Botzinger complex neurons compared to in wild-type mice

decreased miniature excitatory postsynaptic current amplitude ( J:136393 )

• evoked miniature excitatory postsynaptic currents (mEPSCs) are slightly reduced while spontaneous mEPSCs are reduced 30% compared to in wild-type mice

decreased miniature excitatory postsynaptic current frequency ( J:136393 )

• spontaneous mEPSC frequencies are decreased in hippocampal neurons and pre-Botzinger complex neurons compared to in wild-type mice

abnormal neurotransmitter secretion ( J:136393 )

• the probability of vesicular release of the readily releasable pool is reduced 50% compared to in wild-type mice

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

• however, the size of the readily releasable pool is normal

Genotype
MGI:3807187

cx4

• Allelic Composition: Cplx1^tm2Bros/Cplx1^tm2Bros; Cplx2^tm1Bros/Cplx2^tm1Bros; Cplx3^tm1Bros/Cplx3^tm1Bros
• Genetic Background: Not Specified

mortality/aging

neonatal lethality, complete penetrance ( J:136393 )

• mice die within a few hours of birth

nervous system

abnormal pre-Botzinger complex physiology ( J:136393 )

• the amplitude and frequency of spontaneous excitatory postsynaptic currents are decreased in pre-Botzinger complex neurons compared to in wild-type mice

• spontaneous mEPSC frequencies are decreased in hippocampal neurons and pre-Botzinger complex neurons compared to in wild-type

abnormal neuron physiology ( J:136393 )

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

impaired synaptic plasticity ( J:136393 )

• trains of action potentials produce a marked initial facilitation compared to a the depression observed in wild-type mice

decreased excitatory postsynaptic current amplitude ( J:136393 )

• evoked and spontaneous excitatory postsynaptic current amplitudes in hippocampal neurons are reduced compared to in wild-type mice

• in pre-Botzinger complex neurons compared to in wild-type mice

decreased excitatory postsynaptic current frequency ( J:136393 )

• in pre-Botzinger complex neurons compared to in wild-type mice

abnormal inhibitory postsynaptic currents ( J:136393 )

• mice exhibit a reduced evoked inhibitory postsynaptic currents and slightly reduced miniature inhibitory postsynaptic currents compared to wild-type mice

• spontaneous miniature inhibitory postsynaptic current frequencies are decreased in hippocampal and pre-Botzinger complex neurons compared to in wild-type mice

abnormal miniature excitatory postsynaptic currents ( J:136393 )

• evoked miniature excitatory postsynaptic currents (mEPSCs) are slightly reduced while spontaneous mEPSCs are reduced 30% compared to in wild-type mice

• spontaneous mEPSC frequencies are decreased in hippocampal neurons and pre-Botzinger complex neurons compared to in wild-type mice

• the amplitude of spontaneous mEPSC in hippocampal neurons is decreased compared to in wild-type mice

abnormal neurotransmitter secretion ( J:136393 )

• the probability of vesicular release of the readily releasable pool is reduced 50% compared to in wild-type mice

• neuron calcium sensitivity of glutamate and GABA release is reduced compared to in wild-type mice

• however, the size of the readily releasable pool is normal

Genotype
MGI:3807188

cx5

• Allelic Composition: Cplx2^tm1Bros/Cplx2^tm1Bros; Cplx3^tm1Bros/Cplx3^tm1Bros
• Genetic Background: Not Specified

mortality/aging

mortality/aging ( J:136393 )

N • mice are viable

nervous system

nervous system phenotype ( J:136393 )

N • action potential-evoked excitatory and inhibitory postsynaptic currents are normal

N • despite alterations in synaptic transmission, readily releasable pools are normal

reproductive system

reproductive system phenotype ( J:136393 )

N • mice are fertile