Phenotypes associated with this allele

• Allele Symbol: Tsix^tm1Enl
• Allele Name: targeted mutation 1, En Li
• Allele ID: MGI:2679786
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Tsix^tm1Enl/Tsix^+	involves: 129S4/SvJae	MGI:2680289
cx2	Tsix^tm1Enl/Tsix^+ Xist^tm1Jae/Xist^+	involves: 129S4/SvJae	MGI:2680291
cx3	Tsix^tm1Enl/Tsix^+ Xist^tm1Sado/Xist^+	involves: 129S4/SvJae	MGI:3603005
ot4	Tsix^tm1Enl/?	involves: 129S4/SvJae	MGI:2680286

Genotype
MGI:2680289

ht1

• Allelic Composition: Tsix^tm1Enl/Tsix⁺
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

abnormal digestive system physiology ( J:68136 )

♀ • 3-4X increase of apoptotic cells in colons after injury but not when there is no injury

Genotype
MGI:2680291

cx2

• Allelic Composition: Tsix^tm1Enl/Tsix⁺; Xist^tm1Jae/Xist⁺
• Genetic Background: involves: 129S4/SvJae

embryo

decreased embryo size ( J:68136 )

♀ • 7 of 13 embryos were significantly smaller than normal at E8.5 and E9.5

♀ • gastrulation was apparently normal however

♀ • remaining embryos were grossly normal in size and morphology

growth/size/body

decreased embryo size ( J:68136 )

♀ • 7 of 13 embryos were significantly smaller than normal at E8.5 and E9.5

♀ • gastrulation was apparently normal however

♀ • remaining embryos were grossly normal in size and morphology

Genotype
MGI:3603005

cx3

• Allelic Composition: Tsix^tm1Enl/Tsix⁺; Xist^tm1Sado/Xist⁺
• Genetic Background: involves: 129S4/SvJae

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:99399 )

♀ • females that have both mutations on the paternal X chromosome die soon after implantation; however, females that have both mutations on the maternal X chromosome are viable and fertile

Genotype
MGI:2680286

ot4

• Allelic Composition: Tsix^tm1Enl/?
• Genetic Background: involves: 129S4/SvJae

mortality/aging

prenatal lethality, incomplete penetrance ( J:68136 )

• only 1 male and 1 female carrier born out of 104 mice

embryo

abnormal developmental patterning ( J:68136 )

abnormal gastrulation ( J:68136 )

abnormal germ layer development ( J:68136 )

abnormal mesoderm development ( J:68136 )

• at E7.5 mesoderm localized primarily at the embyonic/extraembryonic junction

absent egg cylinders ( J:68136 )

• at E6.5 failed to form well organized egg cylinder although all appropriate primitive tissues present

abnormal ectoplacental cone morphology ( J:68136 )

• at E7.5 the ectoplacental cone cells failed to expand into decidual tissue

abnormal allantois morphology ( J:68136 )

• disorganized at E8.5

small amniotic cavity ( J:68136 )

• at E6.5, the amniotic cavity is small

abnormal chorion morphology ( J:68136 )

• disorganized at E8.5

abnormal ectoplacental cavity morphology ( J:68136 )

• at E7.5, the ectoplacental cavity is small

abnormal extraembryonic coelom morphology ( J:68136 )

• at E7.5, exocoelom small and by E8.5 contains dead cells

abnormal visceral yolk sac morphology ( J:68136 )

• at E8.5, sometimes enlarged and containing disorganized embryonic tissue