Phenotypes associated with this allele

• Allele Symbol: Ticam1^Lps2
• Allele Name: lipopolysaccharide 2
• Allele ID: MGI:2679794
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ticam1^Lps2/Ticam1^Lps2	C57BL/6J-Ticam1^Lps2	MGI:5000268
hm2	Ticam1^Lps2/Ticam1^Lps2	C57BL/6J-Ticam1^Lps2/J	MGI:5520919
hm3	Ticam1^Lps2/Ticam1^Lps2	involves: C57BL/6	MGI:2680622
cx4	Ptpn6^m1Btlr/Ptpn6^m1Btlr Ticam1^Lps2/Ticam1^Lps2	C57BL/6J-Ptpn6^m1Btlr Ticam1^Lps2	MGI:3822471
cx5	Ticam1^Lps2/Ticam1^Lps2 Myd88^tm1Aki/Myd88^tm1Aki	involves: 129P2/OlaHsd * C57BL/6	MGI:2680623
cx6	Irak4^m1Btlr/Irak4^m1Btlr Ticam1^Lps2/Ticam1^Lps2	involves: C57BL/6J	MGI:5437490

Genotype
MGI:5000268

hm1

• Allelic Composition: Ticam1^Lps2/Ticam1^Lps2
• Genetic Background: C57BL/6J-Ticam1^Lps2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Decreased susceptibility to induced colitis in Ticam1^Lps2/Ticam1^Lps2 mice

vision/eye

abnormal retina progenitor cell morphology ( J:141070 )

• more proliferating cells in the retina at 6 days of age

• neuronal differentiation of precursors

nervous system

nervous system phenotype ( J:155613 )

N • no neurological defects after cerebral ischemia/reperfusion (as with controls)

N • infarct size similar to controls

digestive/alimentary system

abnormal enterocyte physiology ( J:167340 )

• flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production of keratinocyte-derived cytokine, macrophage inflammatory protein 3 alpha, and IL-6

decreased susceptibility to induced colitis ( J:167340 )

• significantly improved survival from experimental colitis induced with dextran sodium sulfate and flagellin treatment

• much less histological damage to colon epithelium in induced colitis

• recovery from colitis is improved

immune system

immune system phenotype ( J:131353 )

N • flagellin induction of IFN-beta production is unaffected

decreased susceptibility to induced colitis ( J:167340 )

• significantly improved survival from experimental colitis induced with dextran sodium sulfate and flagellin treatment

• much less histological damage to colon epithelium in induced colitis

• recovery from colitis is improved

abnormal circulating tumor necrosis factor level ( J:138963 )

• ethanol containing diet fails to cause a significant increase in serum TNF as occurs in controls

abnormal cytokine secretion ( J:167340 )

• flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production of keratinocyte-derived cytokine and macrophage inflammatory protein 3 alpha

• IL-1beta strongly induces keratinocyte-derived cytokine production

decreased interleukin-6 secretion ( J:167340 )

• flagellin stimulated primary intestinal epithelial cells display dramatically suppressed production

growth/size/body

abnormal postnatal growth/weight/body size ( J:167340 )

• resistant to weight loss in experimental colitis

liver/biliary system

decreased susceptibility to alcohol-induced hepatic steatosis ( J:138963 )

• fail to develop ethanol induced liver steatosis as occurs in controls

homeostasis/metabolism

abnormal circulating tumor necrosis factor level ( J:138963 )

• ethanol containing diet fails to cause a significant increase in serum TNF as occurs in controls

Genotype
MGI:5520919

hm2

• Allelic Composition: Ticam1^Lps2/Ticam1^Lps2
• Genetic Background: C57BL/6J-Ticam1^Lps2/J

immune system

increased neutrophil cell number ( J:288471 )

• rMA15-SARS-CoV-infected mice show increased recruitment of neutrophils in the lungs at 4 dpi, however by 6 dpi, this is no longer different

increased plasmacytoid dendritic cell number ( J:288471 )

• mice show more plasmacytoid dendritic cells in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

• however, no differences from wild-type are seen in populations of eosinophils, monocyte-derived dendritic cells or in CD11b+ dendritic cell populations, or lymphocytes after rMA15-SARS-CoV infection

decreased CD8-positive, alpha-beta T cell number ( J:288471 )

• mice show fewer CD8+ T cells in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

• however, no differences in the number of CD4+ T cells are seen

increased macrophage cell number ( J:288471 )

• mice show more alveolar macrophages in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

increased Ly6C high monocyte number ( J:288471 )

• mice show more Ly6C high inflammatory monocytes following rMA15-SARS-CoV infection at 4 dpi but not on 6 dpi

decreased Ly6C low monocyte number ( J:288471 )

• mice show similar numbers of Ly6C low monocytes as wild-type mice following rMA15-SARS-CoV infection at 4 dpi but lower numbers at 6 dpi

abnormal chemokine level ( J:288471 )

• levels of chemokines that are chemotactic for neutrophil recruitment (CXCL1, CXCL2, CXCL3) are lower on 2 dpi with rMA15-SARS-CoV but are higher on 4 dpi

increased interferon-beta secretion ( J:288471 )

• rMA15-SARS-CoV-infected mice show increased IFN-beta levels in lung homogenates on 2 and 4 dpi

increased susceptibility to Coronaviridae infection ( J:288471 )

• mice infected intranasally with the mouse-adapted rMA15-SARS-CoV show greater weight loss and continue to lose weight when wild-type mice are recovering, increased viral loads in the lungs and detectable virus at day 6 post-infection when wild-type mice clear the virus

• mice show greater susceptibility to SARS-CoV infection than Tlr3^tm1Flv homozygotes

• rMA15-SARS-CoV-infected mice show more viral antigen in the lungs; more viral antigen is seen in the parenchyma of the lungs but not in the large airways when compared to wild-type mice

• in the large airways, infected cells in the large airways are ciliated airway epithelial cells and are type II pneumocytes in the alveolar spaces

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:288471 )

• rMA15-SARS-CoV-infection leads to lethality

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:288471 )

• rMA15-SARS-CoV-infection leads to lethality

homeostasis/metabolism

abnormal chemokine level ( J:288471 )

• levels of chemokines that are chemotactic for neutrophil recruitment (CXCL1, CXCL2, CXCL3) are lower on 2 dpi with rMA15-SARS-CoV but are higher on 4 dpi

decreased susceptibility to injury ( J:193668 )

• following induction of necrotizing enterocolitis, mice exhibit preservation of the small intestinal mucosa compared with wild-type mice

respiratory system

lung hemorrhage ( J:288471 )

• rMA15-SARS-CoV-infected mice show severe hemorrhage in the lungs at day 6 post-infection

abnormal respiratory function ( J:288471 )

• rMA15-SARS-CoV-infected mice show higher levels of enhanced and infection-associated airway obstruction

increased airway responsiveness ( J:288471 )

• rMA15-SARS-CoV-infected mice show higher levels of enhanced pause indicating airway hyperresponsiveness

abnormal forced expiratory flow rates ( J:288471 )

• rMA15-SARS-CoV-infected mice show higher midtidal expiratory flow

cardiovascular system

lung hemorrhage ( J:288471 )

• rMA15-SARS-CoV-infected mice show severe hemorrhage in the lungs at day 6 post-infection

hematopoietic system

increased neutrophil cell number ( J:288471 )

• rMA15-SARS-CoV-infected mice show increased recruitment of neutrophils in the lungs at 4 dpi, however by 6 dpi, this is no longer different

increased plasmacytoid dendritic cell number ( J:288471 )

• mice show more plasmacytoid dendritic cells in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

• however, no differences from wild-type are seen in populations of eosinophils, monocyte-derived dendritic cells or in CD11b+ dendritic cell populations, or lymphocytes after rMA15-SARS-CoV infection

decreased CD8-positive, alpha-beta T cell number ( J:288471 )

• mice show fewer CD8+ T cells in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

• however, no differences in the number of CD4+ T cells are seen

increased macrophage cell number ( J:288471 )

• mice show more alveolar macrophages in the lungs at 6 dpi, but not 4 dpi, with rMA15-SARS-CoV

increased Ly6C high monocyte number ( J:288471 )

• mice show more Ly6C high inflammatory monocytes following rMA15-SARS-CoV infection at 4 dpi but not on 6 dpi

decreased Ly6C low monocyte number ( J:288471 )

• mice show similar numbers of Ly6C low monocytes as wild-type mice following rMA15-SARS-CoV infection at 4 dpi but lower numbers at 6 dpi

Genotype
MGI:2680622

hm3

• Allelic Composition: Ticam1^Lps2/Ticam1^Lps2
• Genetic Background: involves: C57BL/6

immune system

abnormal macrophage physiology ( J:84896 )

• responses of macrophages to LPS are reduced, with decreased or abolished apoptosis, nitric oxide production, TNF production, and type I interferon production

decreased macrophage apoptosis ( J:84896 , J:92674 )

• greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage (J:92674)

decreased macrophage cytokine production ( J:84896 )

• type I inferferon activity is undetected in culture medium of LPS- or dsRNA-activated macrophages

• no production of type I interferons in response to mouse cytomegalovirus

decreased macrophage nitric oxide production ( J:84896 )

• LPS-induced nitric oxide production by macrophages is abolished

decreased circulating tumor necrosis factor level ( J:86521 )

• 10,000 fold reduction in the TNF alpha response to lipopolysaccharide

abnormal cytokine secretion ( J:110201 )

• cytokine secretion induced by naked polyI:C is severely impaired by 5- to 10-fold

• however, secretion induced by transfected polyI:C is unaffected

decreased tumor necrosis factor secretion ( J:84896 )

• TNF production in response to LPS, synthetic lipid A, and dsRNA is decreased or abolished

decreased susceptibility to endotoxin shock ( J:84896 )

• though some mice become ill and die after intraperitoneal lipopolysaccharide (LPS) injection, the survival rate is significantly greater than that of wild-type

decreased susceptibility to bacterial infection ( J:86521 , J:92674 )

• diminished response to lipopolysaccharide (J:86521)

• 10,000 fold reduction in the TNF alpha response to lipopolysaccharide (J:86521)

• considerable protection against Yersinia enterocolitica induced apoptosis of macrophage (J:92674)

increased susceptibility to Herpesvirales infection ( J:84896 )

• mice are more susceptible to cytomegalovirus infection, with increased lethality and higher viral titers than wild-type mice

• macrophage cultures infected with Vaccinia virus show higher viral titers than control macrophages

homeostasis/metabolism

decreased macrophage nitric oxide production ( J:84896 )

• LPS-induced nitric oxide production by macrophages is abolished

decreased circulating tumor necrosis factor level ( J:86521 )

• 10,000 fold reduction in the TNF alpha response to lipopolysaccharide

cellular

decreased macrophage apoptosis ( J:84896 , J:92674 )

• greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage (J:92674)

hematopoietic system

abnormal macrophage physiology ( J:84896 )

• responses of macrophages to LPS are reduced, with decreased or abolished apoptosis, nitric oxide production, TNF production, and type I interferon production

decreased macrophage apoptosis ( J:84896 , J:92674 )

• greatly reduced apoptosis of Tlr3 and Tlr4 stimulated macrophage (J:92674)

decreased macrophage cytokine production ( J:84896 )

• type I inferferon activity is undetected in culture medium of LPS- or dsRNA-activated macrophages

• no production of type I interferons in response to mouse cytomegalovirus

decreased macrophage nitric oxide production ( J:84896 )

• LPS-induced nitric oxide production by macrophages is abolished

Genotype
MGI:3822471

cx4

• Allelic Composition: Ptpn6^m1Btlr/Ptpn6^m1Btlr; Ticam1^Lps2/Ticam1^Lps2
• Genetic Background: C57BL/6J-Ptpn6^m1Btlr Ticam1^Lps2

limbs/digits/tail

ulcerated autopod ( J:142845 )

• Ticam1 deficiency does not prevent the plantar inflammation that is a phenotypic characteristic for Ptpn6^m1Btlr homozygotes

Genotype
MGI:2680623

cx5

• Allelic Composition: Ticam1^Lps2/Ticam1^Lps2; Myd88^tm1Aki/Myd88^tm1Aki
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

immune system

abnormal macrophage physiology ( J:84896 )

• no response to LPS in vitro

decreased tumor necrosis factor secretion ( J:84896 )

• TNF production by macrophages in response to LPS is abolished

hematopoietic system

abnormal macrophage physiology ( J:84896 )

• no response to LPS in vitro

Genotype
MGI:5437490

cx6

• Allelic Composition: Irak4^m1Btlr/Irak4^m1Btlr; Ticam1^Lps2/Ticam1^Lps2
• Genetic Background: involves: C57BL/6J

immune system

decreased tumor necrosis factor secretion ( J:187583 )

• peritoneal macrophage fail to exhibit a TLR-induced TNFalpha response