Phenotypes associated with this allele

• Allele Symbol: Cnga4^tm1Reed
• Allele Name: targeted mutation 1, Randall R Reed
• Allele ID: MGI:2680015
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cnga4^tm1Reed/Cnga4^tm1Reed	involves: 129	MGI:2680016

Genotype
MGI:2680016

hm1

• Allelic Composition: Cnga4^tm1Reed/Cnga4^tm1Reed
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

taste/olfaction

taste/olfaction phenotype ( J:83225 )

N • homozygotes exhibit normal development of the olfactory epithelium and olfactory bulb relative to wild-type mice

abnormal olfactory system physiology ( J:83225 )

• cAMP-activated currents recorded in inside-out membrane patches excised from dendritic knobs of mutant olfactory receptor neurons (ORNs) indicate a decreased affinity for cAMP, with a dose-response relation shifted by ~10-fold to higher cAMP concentrations

• electro-olfactograms (EOGs) recorded in response to a 500-ms pulse of isobutyl methyl xanthine (IBMX), which increases intracellular cAMP by inhibiting endogenous phosphodiesterase activity, indicate a ~3-fold shift in the IBMX dose-response relation to a higher IBMX concentration

abnormal odor adaptation ( J:83225 )

• homozygotes exhibit a striking reduction in the rate of adaptation of the electrophysiological response to odors in ORNs because of defective Ca²⁺-calmodulin-mediated channel desensitization

• EOG recordings from the olfactory epithelium in response to a long (8-sec) odor stimulus (cineole, 100 M) indicate a significant reduction in the desensitization rate, with similar results obtained in a paired-pulse paradigm (cineole, 100 M, 500 ms) and when IBMX is used as a stimulus

• in response to 50 M Ca²⁺ and 1 M CaM, channels in excised membrane patches from mutant mice display a 70-fold slower Ca²⁺-CaM-induced inhibition along with a several-fold faster current recovery relative to wild-type channels