Phenotypes associated with this allele

• Allele Symbol: Pnlip^tm1Dyh
• Allele Name: targeted mutation 1, David Y Hui
• Allele ID: MGI:2681122
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pnlip^tm1Dyh/Pnlip^tm1Dyh	B6.129-Pnlip^tm1Dyh	MGI:2681123
hm2	Pnlip^tm1Dyh/Pnlip^tm1Dyh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3722846
cx3	Cel^tm1Dyh/Cel^tm1Dyh Pnlip^tm1Dyh/Pnlip^tm1Dyh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3722845

Genotype
MGI:2681123

hm1

• Allelic Composition: Pnlip^tm1Dyh/Pnlip^tm1Dyh
• Genetic Background: B6.129-Pnlip^tm1Dyh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

increased feces cholesterol level ( J:86414 )

• 45% decrease in the amount of cholesterol in feces collected over a 24 hour period, indicating decreased absorption of cholesterol through the intestine

abnormal intestinal lipid absorption ( J:86414 )

• mutants exhibit delayed triglyceride absorption after feeding but no difference in overall fat absorption

decreased intestinal cholesterol absorption ( J:86414 )

• mutants exhibit a significant decrease in the rate of cholesterol absorption as well as the amount of cholesterol absorbed from a single meal

• majority of fat absorption occurs in the distal intestinal segments instead of the proximal intestinal segments as in wild-type, with a significant level occurring in the terminal ileum

• intestinal uptake of cholesterol is significantly decreased in segments 1 and 2 of the intestine but no changes in the distal portions of the intestine when compared with wild-type mice

homeostasis/metabolism

increased feces cholesterol level ( J:86414 )

• 45% decrease in the amount of cholesterol in feces collected over a 24 hour period, indicating decreased absorption of cholesterol through the intestine

abnormal intestinal lipid absorption ( J:86414 )

• mutants exhibit delayed triglyceride absorption after feeding but no difference in overall fat absorption

decreased intestinal cholesterol absorption ( J:86414 )

• mutants exhibit a significant decrease in the rate of cholesterol absorption as well as the amount of cholesterol absorbed from a single meal

• majority of fat absorption occurs in the distal intestinal segments instead of the proximal intestinal segments as in wild-type, with a significant level occurring in the terminal ileum

• intestinal uptake of cholesterol is significantly decreased in segments 1 and 2 of the intestine but no changes in the distal portions of the intestine when compared with wild-type mice

abnormal bile salt homeostasis ( J:86414 )

• mutants exhibit a difference in the composition of minor bile acids, with a decrease in the amount of taurochenodeoxycholate and an increase in taurodeoxycholate compared to wild-type, however no differences in the cholesterol/bile acid ratio

Genotype
MGI:3722846

hm2

• Allelic Composition: Pnlip^tm1Dyh/Pnlip^tm1Dyh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

homeostasis/metabolism

abnormal intestinal lipid absorption ( J:124658 )

• triglyceride absorption is reduced (80.1+/- 3.6%) compared to wild-type (91.5 +/- 0.7%) mice when fed a Western-style diet (high fat)

• using a [¹⁴]C]tripalmitin tracer to determine the levels of triglycerides, long chain retinyl esters and cholesterol absorption, mice have 47% less [¹⁴]C] than in wild-type mice after 9 hours

• using [³]retinyl palmitate mixed in olive oil or in low fat chow, the rate and amount of retinyl ester absorption is reduced

• however, absorption of cholesteryl ester is normal

decreased intestinal cholesterol absorption ( J:124658 )

• when fed a high fat diet, cholesterol absorption is decreased

decreased susceptibility to diet-induced obesity ( J:124658 )

• mice weight less than wild-type counterparts after 10.5 weeks on a high fat diet

decreased circulating insulin level ( J:124658 )

• when fed a high fat diet, fasting insulin levels are lower than in similarly treated wild-type mice

decreased circulating cholesterol level ( J:124658 )

• when fed a high fat diet, plasma cholesterol levels are 36% less than in wild-type mice fed a high fat diet

insulin resistance ( J:124658 )

• mice are more insulin resistant than wild-type mice and Cel^tm1Dyh homozygotes

liver/biliary system

decreased liver weight ( J:124658 )

• liver weight gain associated with consuming a high fat diet is reduced compared to in similarly treated wild-type mice

growth/size/body

increased lean body mass ( J:124658 )

• at 25 weeks of age, mice on a high fat diet have 6.7% more lean body mass than wild-type mice

decreased susceptibility to diet-induced obesity ( J:124658 )

• mice weight less than wild-type counterparts after 10.5 weeks on a high fat diet

adipose tissue

decreased percent body fat/body weight ( J:124658 )

• at 25 weeks of age, mice on a high fat diet have 6.5% less body fat mass than wild-type mice

digestive/alimentary system

abnormal intestinal lipid absorption ( J:124658 )

• triglyceride absorption is reduced (80.1+/- 3.6%) compared to wild-type (91.5 +/- 0.7%) mice when fed a Western-style diet (high fat)

• using a [¹⁴]C]tripalmitin tracer to determine the levels of triglycerides, long chain retinyl esters and cholesterol absorption, mice have 47% less [¹⁴]C] than in wild-type mice after 9 hours

• using [³]retinyl palmitate mixed in olive oil or in low fat chow, the rate and amount of retinyl ester absorption is reduced

• however, absorption of cholesteryl ester is normal

decreased intestinal cholesterol absorption ( J:124658 )

• when fed a high fat diet, cholesterol absorption is decreased

Genotype
MGI:3722845

cx3

• Allelic Composition: Cel^tm1Dyh/Cel^tm1Dyh; Pnlip^tm1Dyh/Pnlip^tm1Dyh
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

homeostasis/metabolism

abnormal intestinal lipid absorption ( J:124658 )

• triglyceride absorption is dramatically reduced (60.1+/- 3.8%) compared to wild-type (91.5 +/- 0.7%) mice and in Pnlip^tm1Dyh homozygotes (80.1+/- 3.6%) when fed a Western-style diet (high fat)

• using a [¹⁴]C]tripalmitin tracer to determine the levels of triglycerides, long chain retinyl esters and cholesterol absorption, mice have 52% less [¹⁴]C] than in wild-type mice after 9 hours

• using [³]retinyl palmitate mixed in olive oil or in low fat chow, the rate and amount of retinyl ester absorption is reduced to a greater extent than in Pnlip^tm1Dyh homozygotes

• using [¹⁴C]cholesteryl oleate, absorption of cholesteryl ester is nearly eliminated as in Cel^tm1Dyh homozygotes

decreased intestinal cholesterol absorption ( J:124658 )

• when fed a high fat diet, cholesterol absorption is decreased similar to in Pnlip^tm1Dyh homozygotes

decreased susceptibility to diet-induced obesity ( J:124658 )

• mice weight less than wild-type counterparts after 0.5 weeks on a high fat diet

decreased circulating insulin level ( J:124658 )

• when fed a high fat diet, fasting insulin levels are lower than in similarly treated wild-type mice

decreased circulating cholesterol level ( J:124658 )

• when fed a high fat diet, plasma cholesterol levels are 45% less than in wild-type mice fed a high fat diet

insulin resistance ( J:124658 )

• mice are more insulin resistant than wild-type mice and Cel^tm1Dyh homozygotes

liver/biliary system

decreased liver weight ( J:124658 )

• liver weight gain associated with consuming a high fat diet is reduced compared to in similarly treated wild-type mice

growth/size/body

increased lean body mass ( J:124658 )

• at 25 weeks of age, mice on a high fat diet have 12.3% more lean body mass than wild-type mice

decreased susceptibility to diet-induced obesity ( J:124658 )

• mice weight less than wild-type counterparts after 0.5 weeks on a high fat diet

adipose tissue

decreased percent body fat/body weight ( J:124658 )

• at 25 weeks of age, mice on a high fat diet have 12.5% less body fat mass than wild-type mice

digestive/alimentary system

abnormal intestinal lipid absorption ( J:124658 )

• triglyceride absorption is dramatically reduced (60.1+/- 3.8%) compared to wild-type (91.5 +/- 0.7%) mice and in Pnlip^tm1Dyh homozygotes (80.1+/- 3.6%) when fed a Western-style diet (high fat)

• using a [¹⁴]C]tripalmitin tracer to determine the levels of triglycerides, long chain retinyl esters and cholesterol absorption, mice have 52% less [¹⁴]C] than in wild-type mice after 9 hours

• using [³]retinyl palmitate mixed in olive oil or in low fat chow, the rate and amount of retinyl ester absorption is reduced to a greater extent than in Pnlip^tm1Dyh homozygotes

• using [¹⁴C]cholesteryl oleate, absorption of cholesteryl ester is nearly eliminated as in Cel^tm1Dyh homozygotes

decreased intestinal cholesterol absorption ( J:124658 )

• when fed a high fat diet, cholesterol absorption is decreased similar to in Pnlip^tm1Dyh homozygotes