Phenotypes associated with this allele

• Allele Symbol: Birc5^tm1Vaux
• Allele Name: targeted mutation 1, David L Vaux
• Allele ID: MGI:2681348
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Birc5^tm1Vaux/Birc5^tm1Vaux	C57BL/6-Birc5^tm1Vaux	MGI:2681389

Genotype
MGI:2681389

hm1

• Allelic Composition: Birc5^tm1Vaux/Birc5^tm1Vaux
• Genetic Background: C57BL/6-Birc5^tm1Vaux

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:86384 )

• homozygous mutant embryos fail to survive beyond E4.5

embryo

failure of blastocyst to hatch from the zona pellucida ( J:86384 )

• all homozygotes fail to hatch out of their zona pellucida

abnormal embryonic tissue morphology ( J:86384 )

• most homozygotes display deterioration of a number of blastomeres at E2.5

• all remaining homozygotes display a relatively normal morphology up to E4.5, but appear grossly abnormal by E5.5

absent blastocoele ( J:86384 )

• by E5.5, homozygotes exhibit gross cellular degeneration and failure to form a blastocoel cavity

inner cell mass degeneration ( J:86384 )

• by E5.5 and E6.5, all remaining homozygotes contain deteriorated inner cell masses

absent trophoblast giant cells ( J:86384 )

• by E5.5 and E6.5, homozygotes contain giant cells rather than spreading trophoblast cells

cellular

abnormal cell nucleus morphology ( J:86384 )

• at E3.5, homozygotes exhibit aberrant nuclear morphology, including micronuclei and some macronuclei

• at E4.5, mutant embryos contain micronuclei and aberrant macronuclei with bridging or lobular morphology, indicating incomplete nuclear fission

• by E5.5, homozygous embryos contain mostly macronuclei with prominent bridging and blebbing morphology

abnormal cell nucleus count ( J:86384 )

• at E3.5, homozygous embryos contain only about half as many nuclei as heterozygous and wild-type embryos

• at E4.5, mutant embryos contain on average less than a third of the number of nuclei detected in heterozygous and wild-type embryos

• by E5.5, each homozygous embryo contains an average of only 13 nuclei whereas heterozygous and wild-type embryos each contain > 200 nuclei

binucleate ( J:86384 )

• at E4.5, mutant embryos contain binucleated or multinucleated cells

abnormal cell cycle ( J:86384 )

• at E2.5, tubulin staining of mutant embryos reveals absence of identifiable intercellular midbodies

• by E4.5, mutant embryos show reduced microtubule networks and highly bundled microtubule spindle cords, indicating aberrant microtubule organization and/or cytokinesis

abnormal mitotic spindle morphology ( J:86384 )

• at E3.5, homozygous mutant embryos lack normal mitotic spindle structures