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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Gcnt1tm1Jxm
targeted mutation 1, Jamey Marth
MGI:2681554
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Gcnt1tm1Jxm/Gcnt1tm1Jxm involves: 129/Sv * C57BL/6 MGI:2681558
hm2
 		Gcnt1tm1Jxm/Gcnt1tm1Jxm involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3703521
cx3
 		Chst4tm1Mifu/Chst4tm1Mifu
Gcnt1tm1Jxm/Gcnt1tm1Jxm 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3703520
cx4
 		B4gat1tm1Mifu/B4gat1tm1Mifu
Gcnt1tm1Jxm/Gcnt1tm1Jxm 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3711068
cx5
 		Gcnt1tm1Jxm/Gcnt1tm1Jxm
Gcnt3tm1.1Jxm/Gcnt3tm1.1Jxm
Gcnt4tm1.1Jxm/Gcnt4tm1.1Jxm 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:3849710


Genotype
MGI:2681558
hm1
Allelic
Composition		 Gcnt1tm1Jxm/Gcnt1tm1Jxm
Genetic
Background		 involves: 129/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gcnt1tm1Jxm mutation (1 available); any Gcnt1 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
behavior/neurological
behavior/neurological phenotype ( J:86387 )
N
• homozygotes lacked overt physical or behavioral abnormalities and were fully fertile

hematopoietic system
abnormal B cell differentiation ( J:86387 )
• B220 epitope absent in splenocytes
increased leukocyte cell number ( J:86387 )
• total WBC count elevated 2.4X
increased neutrophil cell number ( J:86387 )
• accounts for most of WBC count increase
• increased 4.3X

immune system
abnormal B cell differentiation ( J:86387 )
• B220 epitope absent in splenocytes
increased leukocyte cell number ( J:86387 )
• total WBC count elevated 2.4X
increased neutrophil cell number ( J:86387 )
• accounts for most of WBC count increase
• increased 4.3X
abnormal acute inflammation ( J:86387 )
• neutrophil recruitment to inflammation sites reduced in first 4 hours to about 20% of normal




Genotype
MGI:3703521
hm2
Allelic
Composition		 Gcnt1tm1Jxm/Gcnt1tm1Jxm
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gcnt1tm1Jxm mutation (1 available); any Gcnt1 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal leukocyte migration ( J:113195 )
• homing of wild-type lymphocytes to peripheral and mesenteric lymph nodes is reduced to 55 and 85% respectively, relative to wild-type recipient mice
• number of lymphocytes in peripheral lymph nodes is decreased to 78% compared to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• rolling of wild-type lymphocytes on GlyCAM-1 from mutant mice is significantly decreased

cellular
abnormal leukocyte migration ( J:113195 )
• homing of wild-type lymphocytes to peripheral and mesenteric lymph nodes is reduced to 55 and 85% respectively, relative to wild-type recipient mice
• number of lymphocytes in peripheral lymph nodes is decreased to 78% compared to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• rolling of wild-type lymphocytes on GlyCAM-1 from mutant mice is significantly decreased

hematopoietic system
abnormal leukocyte migration ( J:113195 )
• homing of wild-type lymphocytes to peripheral and mesenteric lymph nodes is reduced to 55 and 85% respectively, relative to wild-type recipient mice
• number of lymphocytes in peripheral lymph nodes is decreased to 78% compared to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• rolling of wild-type lymphocytes on GlyCAM-1 from mutant mice is significantly decreased




Genotype
MGI:3703520
cx3
Allelic
Composition		 Chst4tm1Mifu/Chst4tm1Mifu
Gcnt1tm1Jxm/Gcnt1tm1Jxm
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chst4tm1Mifu mutation (0 available); any Chst4 mutation (17 available)
Gcnt1tm1Jxm mutation (1 available); any Gcnt1 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal leukocyte migration ( J:113195 )
• lymphocyte homing to mesenteric and peripheral lymph nodes in double mutant mice is dramatically decreased to ~25% of that in wild-type
• number of lymphocytes in peripheral lymph nodes is decreased to 40% relative to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• GlyCAM-1 from double mutant mice supports the least rolling of wild-type lymphocytes

cellular
abnormal leukocyte migration ( J:113195 )
• lymphocyte homing to mesenteric and peripheral lymph nodes in double mutant mice is dramatically decreased to ~25% of that in wild-type
• number of lymphocytes in peripheral lymph nodes is decreased to 40% relative to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• GlyCAM-1 from double mutant mice supports the least rolling of wild-type lymphocytes

hematopoietic system
abnormal leukocyte migration ( J:113195 )
• lymphocyte homing to mesenteric and peripheral lymph nodes in double mutant mice is dramatically decreased to ~25% of that in wild-type
• number of lymphocytes in peripheral lymph nodes is decreased to 40% relative to wild-type
impaired leukocyte tethering or rolling ( J:113195 )
• GlyCAM-1 from double mutant mice supports the least rolling of wild-type lymphocytes




Genotype
MGI:3711068
cx4
Allelic
Composition		 B4gat1tm1Mifu/B4gat1tm1Mifu
Gcnt1tm1Jxm/Gcnt1tm1Jxm
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
B4gat1tm1Mifu mutation (0 available); any B4gat1 mutation (18 available)
Gcnt1tm1Jxm mutation (1 available); any Gcnt1 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal leukocyte migration ( J:120722 )
• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice
• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice
impaired leukocyte tethering or rolling ( J:120722 )
• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold
• the percentage of cells rolling and sticking is decreased
abnormal lymph node cell ratio ( J:120722 )
• more severe decrease in the number of lymph-node resident B cells compared to single homozygotes
• however, no change is detected in the total number of lymphocytes found in secondary lymphoid tissues
decreased susceptibility to type IV hypersensitivity reaction ( J:120722 )
• when sensitized and challenged with dinitrofluorobenzee (DNFB) mice exhibit 37% less ear swelling and have less CD3+ T lymphocyte infiltration than wild-type mice

cellular
abnormal leukocyte migration ( J:120722 )
• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice
• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice
impaired leukocyte tethering or rolling ( J:120722 )
• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold
• the percentage of cells rolling and sticking is decreased

hematopoietic system
abnormal leukocyte migration ( J:120722 )
• homing is 45% and 65% of wild-type mice in 2-hour and 20-hour assays, respectively, and is not inhibited by MECA-79 unlike in wild-type mice
• after challenge with dinitrofluorobenzene homing of lymphocytes to draining lymph nodes and lymphocyte numbers are reduced by 37% compared to wild-type mice
impaired leukocyte tethering or rolling ( J:120722 )
• rolling velocities of T lymphocytes in venules (orders III and IV) are increased about 3-fold
• the percentage of cells rolling and sticking is decreased




Genotype
MGI:3849710
cx5
Allelic
Composition		 Gcnt1tm1Jxm/Gcnt1tm1Jxm
Gcnt3tm1.1Jxm/Gcnt3tm1.1Jxm
Gcnt4tm1.1Jxm/Gcnt4tm1.1Jxm
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gcnt1tm1Jxm mutation (1 available); any Gcnt1 mutation (30 available)
Gcnt3tm1.1Jxm mutation (0 available); any Gcnt3 mutation (25 available)
Gcnt4tm1.1Jxm mutation (0 available); any Gcnt4 mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
abnormal intestinal mucosa morphology ( J:149909 )
• gastric/intestinal tissues lack all core 2 O-glycan structures with a corresponding increase in various core 1 O-glycan structures

renal/urinary system
abnormal kidney morphology ( J:149909 )
• kidney tissues lack all core 2 O-glycan structures with a corresponding increase in various core 1 O-glycan structures




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory