Phenotypes associated with this allele

• Allele Symbol: Rag1^tm1Fwa
• Allele Name: targeted mutation 1, Frederick W Alt
• Allele ID: MGI:2681693
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rag1^tm1Fwa/Rag1^tm1Fwa	involves: 129	MGI:2681718
cx2	Cmtm7^tm2a(EUCOMM)Hmgu/Cmtm7^+ Rag1^tm1Fwa/Rag1^tm1Fwa	chimera involves: C57BL/6 * C57BL/6N	MGI:6100917
cx3	Rag1^tm1Fwa/Rag1^tm1Fwa Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * 129S6/SvEvTac	MGI:5002826

Genotype
MGI:2681718

hm1

• Allelic Composition: Rag1^tm1Fwa/Rag1^tm1Fwa
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus hypoplasia ( J:86464 )

• reduced thymic cellularity

abnormal B cell differentiation ( J:86464 )

• transition from pro-B to pre-B modestly impaired

• reduced overall level of heavy chain VDJ rearrangements in pro-B cells

• order in which heavy chain VDJ rearrangements occur is frequently abnormal

abnormal T cell differentiation ( J:86464 )

• transition from pro-T to pre-T cells is impaired

increased double-negative T cell number ( J:86464 )

• high percentage of double negative pro-T cells, particularly CD25+CD44- (DN3)stage

• approximately normal distribution of double positive and single positive cells

decreased B cell number ( J:86464 )

• splenic B cells reduced about 50%

hematopoietic system

thymus hypoplasia ( J:86464 )

• reduced thymic cellularity

abnormal B cell differentiation ( J:86464 )

• transition from pro-B to pre-B modestly impaired

• reduced overall level of heavy chain VDJ rearrangements in pro-B cells

• order in which heavy chain VDJ rearrangements occur is frequently abnormal

abnormal T cell differentiation ( J:86464 )

• transition from pro-T to pre-T cells is impaired

increased double-negative T cell number ( J:86464 )

• high percentage of double negative pro-T cells, particularly CD25+CD44- (DN3)stage

• approximately normal distribution of double positive and single positive cells

decreased B cell number ( J:86464 )

• splenic B cells reduced about 50%

endocrine/exocrine glands

thymus hypoplasia ( J:86464 )

• reduced thymic cellularity

Genotype
MGI:6100917

cx2

• Allelic Composition: Cmtm7^{tm2a(EUCOMM)Hmgu}/Cmtm7⁺; Rag1^tm1Fwa/Rag1^tm1Fwa
• Genetic Background: chimera involves: C57BL/6 * C57BL/6N
• Cell Lines: HEPD0787_6_G03

hematopoietic system

decreased plasma cell number ( J:207033 )

• greatly reduced frequency of IgM-secreting plasma cells

abnormal B-1a B cell morphology ( J:207033 )

• B-1a cells expressed significantly lower levels of BCR

decreased B-1a cell number ( J:207033 )

• B-1a cell population was dramatically reduced in the peritoneal caivty

abnormal B cell physiology ( J:207033 )

• impaired survival of B-1a cells

decreased B cell proliferation ( J:207033 )

• B-1a and B-1b cells show reduced proliferation

decreased IgG2b level ( J:207033 )

decreased IgM level ( J:207033 )

immune system

decreased plasma cell number ( J:207033 )

• greatly reduced frequency of IgM-secreting plasma cells

abnormal B-1a B cell morphology ( J:207033 )

• B-1a cells expressed significantly lower levels of BCR

decreased B-1a cell number ( J:207033 )

• B-1a cell population was dramatically reduced in the peritoneal caivty

abnormal B cell physiology ( J:207033 )

• impaired survival of B-1a cells

decreased B cell proliferation ( J:207033 )

• B-1a and B-1b cells show reduced proliferation

decreased IgG2b level ( J:207033 )

decreased IgM level ( J:207033 )

cellular

decreased B cell proliferation ( J:207033 )

• B-1a and B-1b cells show reduced proliferation

Genotype
MGI:5002826

cx3

• Allelic Composition: Rag1^tm1Fwa/Rag1^tm1Fwa; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac

cellular

cellular phenotype ( J:170462 )

N • no genomic instability is observed