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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cdc25btm1Pjd
targeted mutation 1, Peter J Donovan
MGI:2682953
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cdc25btm1Pjd/Cdc25btm1Pjd Not Specified MGI:2682957
cn2
 		Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Cdc25ctm1Hpw/Cdc25ctm1Hpw
Gt(ROSA)26Sortm1(cre/ERT)Brn/Gt(ROSA)26Sor+ 		involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 MGI:3845392
cn3
 		Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Tg(Vil1-cre/ERT2)23Syr/0 		involves: 129X1/SvJ * C57BL/6 * DBA/2 MGI:4941917
cn4
 		Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Cdc25ctm1Hpw/Cdc25ctm1Hpw
Tg(Vil1-cre/ERT2)23Syr/0 		involves: 129X1/SvJ * C57BL/6 * DBA/2 MGI:4941918
cn5
 		Cdc25btm1Hpw/Cdc25btm1Pjd
Tg(Vil1-cre/ERT2)23Syr/0 		involves: 129X1/SvJ * C57BL/6 * DBA/2 MGI:4943329


Genotype
MGI:2682957
hm1
Allelic
Composition		 Cdc25btm1Pjd/Cdc25btm1Pjd
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc25btm1Pjd mutation (0 available); any Cdc25b mutation (30 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal oocyte morphology ( J:87011 )
• MPF is not activated in oocytes and they do not undergo germinal vesicle breakdown
• folliculogenesis and ovulation were unimpaired
abnormal female meiosis ( J:87011 )
• while spermatogenesis was unaffected, the female meiotic cycle was arrested at prophase I due to an inability to activate maturation-promoting factor (MPF)

cellular
abnormal oocyte morphology ( J:87011 )
• MPF is not activated in oocytes and they do not undergo germinal vesicle breakdown
• folliculogenesis and ovulation were unimpaired
abnormal female meiosis ( J:87011 )
• while spermatogenesis was unaffected, the female meiotic cycle was arrested at prophase I due to an inability to activate maturation-promoting factor (MPF)




Genotype
MGI:3845392
cn2
Allelic
Composition		 Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Cdc25ctm1Hpw/Cdc25ctm1Hpw
Gt(ROSA)26Sortm1(cre/ERT)Brn/Gt(ROSA)26Sor+
Genetic
Background		 involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc25atm1.1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25atm1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25btm1Pjd mutation (0 available); any Cdc25b mutation (30 available)
Cdc25ctm1Hpw mutation (1 available); any Cdc25c mutation (23 available)
Gt(ROSA)26Sortm1(cre/ERT)Brn mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Shortened villi in the small intestine of Cdc25atm1Hpw/Cdc25atm1.1Hpw Cdc25btm1Pjd/Cdc25btm1Pjd Cdc25ctm1Hpw/Cdc25ctm1Hpw Gt(ROSA)26Sortm1(cre/ERT)Brn/Gt(ROSA)26Sor+ (TKO) mice

mortality/aging
premature death ( J:145768 )
• adult mice die within 1 week of initial 4-hydroxytamoxifen (OHT) injection

growth/size/body
weight loss ( J:145768 )
• adult mice exhibit significant weight loss (20%) within 1 week of initial tamoxifen injection

digestive/alimentary system
abnormal small intestine crypts of Lieberkuhn morphology ( J:145768 )
• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth
• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed
• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts
• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells
abnormal small intestinal villus morphology ( J:145768 )
• villi are lined with shorter, less-dense enterocytes in proximal regions; complete loss of distal villi is observed in some areas
decreased small intestinal villus height ( J:145768 )
• significant loss of villus height in proximal and distal regions is observed in mutants after OHT treatment
decreased small intestine length ( J:145768 )
• 6 days following the final of 3 consecutive injections of tamoxifen, length of small intestine is shortened by 40% compared to controls

endocrine/exocrine glands
abnormal small intestine crypts of Lieberkuhn morphology ( J:145768 )
• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth
• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed
• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts
• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells




Genotype
MGI:4941917
cn3
Allelic
Composition		 Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Tg(Vil1-cre/ERT2)23Syr/0
Genetic
Background		 involves: 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc25atm1.1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25atm1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25btm1Pjd mutation (0 available); any Cdc25b mutation (30 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:169457 )
• 2 of 5 tamoxifen-treated mice die by day 8

digestive/alimentary system
increased enterocyte apoptosis ( J:169457 )
• in tamoxifen-treated mice
abnormal enterocyte differentiation ( J:169457 )
• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
abnormal small intestinal crypt cell proliferation ( J:169457 )
• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
abnormal large intestine morphology ( J:169457 )
• reduced length in tamoxifen-treated mice
abnormal small intestine morphology ( J:169457 )
• reduced length in tamoxifen-treated mice
abnormal small intestine crypts of Lieberkuhn morphology ( J:169457 )
• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
decreased small intestinal villus size ( J:169457 )
• in tamoxifen-treated mice

growth/size/body
decreased body weight ( J:169457 )
• in tamoxifen-treated mice

endocrine/exocrine glands
abnormal small intestine crypts of Lieberkuhn morphology ( J:169457 )
• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal

cellular
increased enterocyte apoptosis ( J:169457 )
• in tamoxifen-treated mice
abnormal enterocyte differentiation ( J:169457 )
• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
abnormal small intestinal crypt cell proliferation ( J:169457 )
• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice




Genotype
MGI:4941918
cn4
Allelic
Composition		 Cdc25atm1Hpw/Cdc25atm1.1Hpw
Cdc25btm1Pjd/Cdc25btm1Pjd
Cdc25ctm1Hpw/Cdc25ctm1Hpw
Tg(Vil1-cre/ERT2)23Syr/0
Genetic
Background		 involves: 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc25atm1.1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25atm1Hpw mutation (1 available); any Cdc25a mutation (34 available)
Cdc25btm1Pjd mutation (0 available); any Cdc25b mutation (30 available)
Cdc25ctm1Hpw mutation (1 available); any Cdc25c mutation (23 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
postnatal lethality, incomplete penetrance ( J:169457 )
• 5 of 11 tamoxifen-treated mice die by day 8

digestive/alimentary system
increased enterocyte apoptosis ( J:169457 )
• in tamoxifen-treated mice
abnormal enterocyte differentiation ( J:169457 )
• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
abnormal small intestinal crypt cell proliferation ( J:169457 )
• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice
abnormal large intestine morphology ( J:169457 )
• reduced length in tamoxifen-treated mice
abnormal small intestine morphology ( J:169457 )
• reduced length in tamoxifen-treated mice
abnormal small intestine crypts of Lieberkuhn morphology ( J:169457 )
• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal
decreased small intestinal villus size ( J:169457 )
• in tamoxifen-treated mice

growth/size/body
decreased body weight ( J:169457 )
• in tamoxifen-treated mice

endocrine/exocrine glands
abnormal small intestine crypts of Lieberkuhn morphology ( J:169457 )
• tamoxifen treated mice exhibit a reduction in epithelium cells in the small intestine crypts compared with control mice
• however, the number of Paneth cells is normal

cellular
increased enterocyte apoptosis ( J:169457 )
• in tamoxifen-treated mice
abnormal enterocyte differentiation ( J:169457 )
• tamoxifen-treated mice exhibit premature differentiation of small intestine crypt cells compared with control mice
abnormal small intestinal crypt cell proliferation ( J:169457 )
• tamoxifen-treated mice exhibit decreased S-phase cells in crypts compared with control mice




Genotype
MGI:4943329
cn5
Allelic
Composition		 Cdc25btm1Hpw/Cdc25btm1Pjd
Tg(Vil1-cre/ERT2)23Syr/0
Genetic
Background		 involves: 129X1/SvJ * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cdc25btm1Hpw mutation (1 available); any Cdc25b mutation (30 available)
Cdc25btm1Pjd mutation (0 available); any Cdc25b mutation (30 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
digestive/alimentary system
digestive/alimentary phenotype ( J:169457 )
N
• tamoxifen-treated mice exhibit normal intestine morphology

growth/size/body
growth/size/body region phenotype ( J:169457 )
N
• tamoxifen-treated mice exhibit normal body weight




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory