Phenotypes associated with this allele

• Allele Symbol: Itpkc^tm1Ssch
• Allele Name: targeted mutation 1, Stephane Schurmans
• Allele ID: MGI:2683901
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Itpkc^tm1Ssch/Itpkc^tm1Ssch	involves: 129S1/Sv * 129X1/SvJ	MGI:2683923
cx2	Itpkb^tm1Ssch/Itpkb^tm1Ssch Itpkc^tm1Ssch/Itpkc^tm1Ssch	involves: 129S1/Sv * 129X1/SvJ * CD-1	MGI:2683926

Genotype
MGI:2683923

hm1

• Allelic Composition: Itpkc^tm1Ssch/Itpkc^tm1Ssch
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:86257 )

• homozygotes exhibit normal thymocyte development

Genotype
MGI:2683926

cx2

• Allelic Composition: Itpkb^tm1Ssch/Itpkb^tm1Ssch; Itpkc^tm1Ssch/Itpkc^tm1Ssch
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * CD-1

immune system

abnormal thymocyte activation ( J:86257 )

• after stimulation of thymocytes with concanavalin A or anti-CD3 antibody, double homozygotes display significantly reduced concentrations of Ins(1,3,4,5)P4 relative to similarly-treated controls

• however, no defects in calcium mobilization are observed following stimulation of double mutant thymocytes with ionomycin or CD3 mAb treatment, similar to single Itpkb^tm1Ssch homozygotes

arrested T cell differentiation ( J:86257 )

• double homozygotes display a developmental block between the double-positive and mature single-positive thymocyte stage, similar to that observed in single Itpkb^tm1Ssch homozygotes

absent CD4-positive, alpha-beta T cells ( J:86257 )

• double homozygotes display near absence of mature CD4⁺ T cells in lymph nodes and spleen, similar to that observed in single Itpkb^tm1Ssch homozygotes

absent CD8-positive, alpha-beta T cells ( J:86257 )

• double homozygotes display near absence of mature CD8⁺ T cells in lymph nodes and spleen, similar to that observed in single Itpkb^tm1Ssch homozygotes

hematopoietic system

abnormal thymocyte activation ( J:86257 )

• after stimulation of thymocytes with concanavalin A or anti-CD3 antibody, double homozygotes display significantly reduced concentrations of Ins(1,3,4,5)P4 relative to similarly-treated controls

• however, no defects in calcium mobilization are observed following stimulation of double mutant thymocytes with ionomycin or CD3 mAb treatment, similar to single Itpkb^tm1Ssch homozygotes

arrested T cell differentiation ( J:86257 )

• double homozygotes display a developmental block between the double-positive and mature single-positive thymocyte stage, similar to that observed in single Itpkb^tm1Ssch homozygotes

absent CD4-positive, alpha-beta T cells ( J:86257 )

• double homozygotes display near absence of mature CD4⁺ T cells in lymph nodes and spleen, similar to that observed in single Itpkb^tm1Ssch homozygotes

absent CD8-positive, alpha-beta T cells ( J:86257 )

• double homozygotes display near absence of mature CD8⁺ T cells in lymph nodes and spleen, similar to that observed in single Itpkb^tm1Ssch homozygotes

endocrine/exocrine glands

abnormal thymocyte activation ( J:86257 )

• after stimulation of thymocytes with concanavalin A or anti-CD3 antibody, double homozygotes display significantly reduced concentrations of Ins(1,3,4,5)P4 relative to similarly-treated controls

• however, no defects in calcium mobilization are observed following stimulation of double mutant thymocytes with ionomycin or CD3 mAb treatment, similar to single Itpkb^tm1Ssch homozygotes