Phenotypes associated with this allele

• Allele Symbol: Mcl1^tm3Sjk
• Allele Name: targeted mutation 3, Stanley J Korsmeyer
• Allele ID: MGI:2684151
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mcl1^tm3Sjk/Mcl1^tm3Sjk	involves: 129X1/SvJ	MGI:2684173
cn2	Cd19^tm1(cre)Cgn/Cd19^+ Mcl1^tm2Sjk/Mcl1^tm3Sjk	involves: 129P2/OlaHsd * 129X1/SvJ	MGI:2684157
cn3	Mcl1^tm3Sjk/Mcl1^tm3Sjk Tg(Cpa3-cre)3Glli/0	involves: 129X1/Sv * C57BL/6	MGI:5301529
cn4	Mcl1^tm2Sjk/Mcl1^tm3Sjk Tg(Mx1-cre)1Cgn/0	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:2684174
cn5	Mcl1^tm2Sjk/Mcl1^tm3Sjk Tg(Lck-cre)1Cwi/0	involves: 129X1/SvJ * C57BL/6 * DBA/2	MGI:2684156

Genotype
MGI:2684173

hm1

• Allelic Composition: Mcl1^tm3Sjk/Mcl1^tm3Sjk
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:86906 )

• mice were viable and born at the expected Mendelian frequency

Genotype
MGI:2684157

cn2

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Mcl1^tm2Sjk/Mcl1^tm3Sjk
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ

hematopoietic system

increased B cell apoptosis ( J:86906 )

• increased apoptosis at pro-B cell stage

abnormal B cell differentiation ( J:86906 )

absent early pro-B cells ( J:86906 )

absent late pro-B cells ( J:86906 )

arrested B cell differentiation ( J:86906 )

• arrested at pro-B cell stage

decreased B cell number ( J:86906 )

• decrease in peripheral B cells

• 88% reduction of splenic B cells relative to controls

• near absence of lymph node B cells

• detected peripheral B cells did not undergo cre-mediated recombination

absent pre-B cells ( J:86906 )

immune system

increased B cell apoptosis ( J:86906 )

• increased apoptosis at pro-B cell stage

abnormal B cell differentiation ( J:86906 )

absent early pro-B cells ( J:86906 )

absent late pro-B cells ( J:86906 )

arrested B cell differentiation ( J:86906 )

• arrested at pro-B cell stage

decreased B cell number ( J:86906 )

• decrease in peripheral B cells

• 88% reduction of splenic B cells relative to controls

• near absence of lymph node B cells

• detected peripheral B cells did not undergo cre-mediated recombination

absent pre-B cells ( J:86906 )

cellular

increased B cell apoptosis ( J:86906 )

• increased apoptosis at pro-B cell stage

Genotype
MGI:5301529

cn3

• Allelic Composition: Mcl1^tm3Sjk/Mcl1^tm3Sjk; Tg(Cpa3-cre)3Glli/0
• Genetic Background: involves: 129X1/Sv * C57BL/6

hematopoietic system

hematopoietic system phenotype ( J:179080 )

N • eosinophil numbers in blood, spleen, and bone marrow are similar to controls, while neutrophil numbers in blood, bone marrow and peritoneal cavity are also similar to controls; a modest increase in neutrophils is observed in the spleen

N • no signfiicant changes in T cell or B cell number are detected; absolute numbers of lymphocytes or myeloid cells in the blood are not significantly changed

macrocytic anemia ( J:179080 )

• mice exhibit a mild macrocytic anemia

decreased basophil cell number ( J:179080 )

• significantly fewer basphils are observed in the spleen, bone marrow, and blood of 3-10 month-old mice compared with controls

decreased erythrocyte cell number ( J:179080 )

• mice show approximately a 31% reduction in erythrocytes in blood

decreased hematocrit ( J:179080 )

• a 24% reduction relative to controls is observed

decreased mast cell number ( J:179080 )

• 6 week to 10 month-old mice show significant reductions in mast cell numbers in almost all tissues and organs examined, except in the spleen

immune system

decreased basophil cell number ( J:179080 )

• significantly fewer basphils are observed in the spleen, bone marrow, and blood of 3-10 month-old mice compared with controls

decreased mast cell number ( J:179080 )

• 6 week to 10 month-old mice show significant reductions in mast cell numbers in almost all tissues and organs examined, except in the spleen

decreased inflammatory response ( J:179080 )

• IgE-dependent chronic allergic inflammation (CAI) is reduced; leukocyte infiltration into the dermis is almost absent and basophils are not detected 3 days after IgE challenge

decreased susceptibility to type I hypersensitivity reaction ( J:179080 )

• in IgE-treated mice, IgE-dependent passive cutaneous anaphylaxis (PCA) is reduced compared to controls; tissue swelling is reduced relative to treated controls at 30 minutes after antigen challenge

• mast cell numbers in dermis of ear pinnae 6 hours after treatment with IgE are low and no detectable increase in dermal leukocytes is observed

• in IgE-dependent passive systemic anaphylaxis (PSA), IgE-sensitized mutants are essentially un reactive to challenge with IgE injection, whereas controls exhibit hypothermia and sometimes death in response to identical challenge

Genotype
MGI:2684174

cn4

• Allelic Composition: Mcl1^tm2Sjk/Mcl1^tm3Sjk; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

hematopoietic system

decreased B cell number ( J:86906 )

• peripheral B cells are not maintained

decreased T cell number ( J:86906 )

• peripheral T cells are not maintained

immune system

decreased B cell number ( J:86906 )

• peripheral B cells are not maintained

decreased T cell number ( J:86906 )

• peripheral T cells are not maintained

Genotype
MGI:2684156

cn5

• Allelic Composition: Mcl1^tm2Sjk/Mcl1^tm3Sjk; Tg(Lck-cre)1Cwi/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA/2

hematopoietic system

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

arrested T cell differentiation ( J:86906 )

• block at the double-negative stage between DN2 and DN3

decreased T cell number ( J:86906 )

• decreased number of peripheral T cells

• detected peripheral T cells did not undergo cre-mediated recombination

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes

decreased double-negative T cell number ( J:86906 )

• reduction of double-negative cells in DN3 and DN4 subsets, but not in DN1 or DN2 subsets

decreased double-positive T cell number ( J:86906 )

immune system

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

arrested T cell differentiation ( J:86906 )

• block at the double-negative stage between DN2 and DN3

decreased T cell number ( J:86906 )

• decreased number of peripheral T cells

• detected peripheral T cells did not undergo cre-mediated recombination

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes

decreased double-negative T cell number ( J:86906 )

• reduction of double-negative cells in DN3 and DN4 subsets, but not in DN1 or DN2 subsets

decreased double-positive T cell number ( J:86906 )

cellular

increased T cell apoptosis ( J:86906 )

• increased apoptosis at double negative T cell stage

endocrine/exocrine glands

decreased thymocyte number ( J:86906 )

• reduction of the total number of thymocytes