Phenotypes associated with this allele

• Allele Symbol: Rac1^tm1Tyb
• Allele Name: targeted mutation 1, Victor L J Tybulewicz
• Allele ID: MGI:2684419
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd19^tm1(cre)Cgn/Cd19^+ Rac1^tm1Tyb/Rac1^tm1Tyb	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:2684441
cn2	Cd19^tm1(cre)Cgn/Cd19^+ Rac1^tm1Tyb/Rac1^tm1Tyb Rac2^tm1Mddw/Rac2^tm1Mddw	involves: 129P2/OlaHsd * 129S/SvEv * 129S4/SvJae * C57BL/6	MGI:2684442
cn3	Rac1^tm1Tyb/Rac1^tm2Tyb Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S4/SvJae * C3H * C57BL/6 * CBA	MGI:5050115
cn4	Rac1^tm1Tyb/Rac1^tm2Tyb Tg(Ttr-cre)1Hadj/0	involves: 129S4/SvJae * C3H * C57BL/6 * CBA/J	MGI:5050120
cn5	Pten^tm1Ppp/Pten^+ Rac1^tm1Tyb/Rac1^tm2Tyb Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S/Sv * C3H * C57BL/6 * CBA	MGI:5050118

Genotype
MGI:2684441

cn1

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Rac1^tm1Tyb/Rac1^tm1Tyb
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:86765 )

N • B lineage cells were normal in bone marrow, spleen, and lymph nodes

Genotype
MGI:2684442

cn2

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Rac1^tm1Tyb/Rac1^tm1Tyb; Rac2^tm1Mddw/Rac2^tm1Mddw
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * 129S4/SvJae * C57BL/6

hematopoietic system

arrested B cell differentiation ( J:86765 )

• development blocked at the T1 stage

decreased B cell number ( J:86765 )

decreased transitional stage B cell number ( J:86765 )

• diminished splenic T1 and T2 cells

decreased B-1 B cell number ( J:86765 )

• peritoneal B cells (B1) also reduced

decreased follicular B cell number ( J:86765 )

• diminished recirculating follicular B cells

decreased marginal zone B cell number ( J:86765 )

• splenic marginal B cells were reduced

immune system

arrested B cell differentiation ( J:86765 )

• development blocked at the T1 stage

decreased B cell number ( J:86765 )

decreased transitional stage B cell number ( J:86765 )

• diminished splenic T1 and T2 cells

decreased B-1 B cell number ( J:86765 )

• peritoneal B cells (B1) also reduced

decreased follicular B cell number ( J:86765 )

• diminished recirculating follicular B cells

decreased marginal zone B cell number ( J:86765 )

• splenic marginal B cells were reduced

Genotype
MGI:5050115

cn3

• Allelic Composition: Rac1^tm1Tyb/Rac1^tm2Tyb; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6 * CBA

mortality/aging

prenatal lethality, complete penetrance ( J:166095 )

• survive longer than germ line null mice

• survive until E8.5

embryo

increased embryonic tissue cell apoptosis ( J:173526 )

• in the mesoderm and basal regions of the epiblast at E7.5

• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half

abnormal developmental patterning ( J:173526 )

• expression analysis indicates organization of midline structures is abnormal

• the midline is broad at E8.5

abnormal mesoderm development ( J:173526 )

• mesoderm cells are specified but migration away from the primitive streak is impaired

• mesodermal cells are round, with just a few short protrusions

decreased embryo size ( J:173526 )

• the embryonic region is smaller compared to control littermates from E7.5 onwards

abnormal notochordal plate morphology ( J:173526 )

• expression analysis indicates the notochordal plate is wider and shorter at E7.75

abnormal neural tube closure ( J:173526 )

• the neural plate is present but fails to initiate neural tube closure

abnormal primitive streak morphology ( J:173526 )

• during epithelial to mesenchymal transition in the primitive streak breakdown of the basement membrane appears somewhat disrupted with large laminin aggregates seen on nascent mesodermal cells

• accumulation of T expressing cells in the primitive streak indicating an impairment in migration of cells away from the streak

abnormal primitive node morphology ( J:173526 )

• node cells are specified but fail to coalesce to form a normal node

impaired somite development ( J:173526 )

• properly organized somites do not form

cellular

abnormal cell adhesion ( J:173526 )

• cultured cells from E7.5 embryos fail to adhere to either fibronectin or Matrigel matrices

increased embryonic tissue cell apoptosis ( J:173526 )

• in the mesoderm and basal regions of the epiblast at E7.5

• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half

decreased cell proliferation ( J:173526 )

• decrease in the mitotic index in E7.5 embryos

cardiovascular system

cardia bifida ( J:173526 )

growth/size/body

decreased embryo size ( J:173526 )

• the embryonic region is smaller compared to control littermates from E7.5 onwards

nervous system

abnormal neural tube closure ( J:173526 )

• the neural plate is present but fails to initiate neural tube closure

Genotype
MGI:5050120

cn4

• Allelic Composition: Rac1^tm1Tyb/Rac1^tm2Tyb; Tg(Ttr-cre)1Hadj/0
• Genetic Background: involves: 129S4/SvJae * C3H * C57BL/6 * CBA/J

mortality/aging

embryonic lethality, complete penetrance ( J:166095 )

• survive longer than germ line null mice, up to E8.25

embryo

abnormal anterior visceral endoderm cell migration ( J:166095 )

• migration of anterior visceral endoderm cells is disrupted

• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression

abnormal rostral-caudal axis patterning ( J:166095 )

• expression analysis indicates a defect in the initial specification of the AP axis in most embryos

embryonic-extraembryonic boundary constriction ( J:166095 )

• constriction at the boundary between embryonic and extraembryonic regions

abnormal extraembryonic endoderm formation ( J:173526 )

• visceral endoderm cells fail to form a border around the midline structures

craniofacial

abnormal craniofacial development ( J:166095 )

• abnormal headfolds at E7.5

cellular

abnormal anterior visceral endoderm cell migration ( J:166095 )

• migration of anterior visceral endoderm cells is disrupted

• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression

Genotype
MGI:5050118

cn5

• Allelic Composition: Pten^tm1Ppp/Pten⁺; Rac1^tm1Tyb/Rac1^tm2Tyb; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S/Sv * C3H * C57BL/6 * CBA

embryo

abnormal embryo development ( J:173526 )

• embryo morphology is stated to be indistinguishable from that of mutant mice wild-type for Pten

cellular

abnormal apoptosis ( J:173526 )

• cell death is reduced compared to mutant mice wild-type for Pten