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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Rac1tm2Tyb
targeted mutation 2, Victor L J Tybulewicz
MGI:2684421
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Rac1tm2Tyb/Rac1tm2Tyb involves: 129S4/SvJae * C3H * C57BL/6 * CD-1 MGI:5050121
hm2
Rac1tm2Tyb/Rac1tm2Tyb involves: 129S4/SvJae * C57BL/6 MGI:2684445
cn3
Rac1tm1Tyb/Rac1tm2Tyb
Edil3Tg(Sox2-cre)1Amc/Edil3+
involves: 129S4/SvJae * C3H * C57BL/6 * CBA MGI:5050115
cn4
Rac1tm1Tyb/Rac1tm2Tyb
Tg(Ttr-cre)1Hadj/0
involves: 129S4/SvJae * C3H * C57BL/6 * CBA/J MGI:5050120
cn5
Ptentm1Ppp/Pten+
Rac1tm1Tyb/Rac1tm2Tyb
Edil3Tg(Sox2-cre)1Amc/Edil3+
involves: 129S/Sv * C3H * C57BL/6 * CBA MGI:5050118
cx6
Rac1tm2Tyb/Rac1tm2Tyb
Tg(Hhex-EGFP)#Rbe/0
mixed MGI:5050122


Genotype
MGI:5050121
hm1
Allelic
Composition
Rac1tm2Tyb/Rac1tm2Tyb
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6 * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

embryo
• expression analysis indicates anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
• most arrest before E7.5 and all arrest by E7.5
• primitive streak formation is ectopically initiated at all positions around the circumference of most embryos
• constriction at the boundary between embryonic and extraembryonic regions in mice that survive to E7.5

cellular
• expression analysis indicates anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5




Genotype
MGI:2684445
hm2
Allelic
Composition
Rac1tm2Tyb/Rac1tm2Tyb
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygous embryos disappear by E11.5




Genotype
MGI:5050115
cn3
Allelic
Composition
Rac1tm1Tyb/Rac1tm2Tyb
Edil3Tg(Sox2-cre)1Amc/Edil3+
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Edil3Tg(Sox2-cre)1Amc mutation (5 available); any Edil3 mutation (43 available)
Rac1tm1Tyb mutation (0 available); any Rac1 mutation (24 available)
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• survive longer than germ line null mice
• survive until E8.5

embryo
• in the mesoderm and basal regions of the epiblast at E7.5
• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half
• expression analysis indicates organization of midline structures is abnormal
• the midline is broad at E8.5
• mesoderm cells are specified but migration away from the primitive streak is impaired
• mesodermal cells are round, with just a few short protrusions
• the embryonic region is smaller compared to control littermates from E7.5 onwards
• expression analysis indicates the notochordal plate is wider and shorter at E7.75
• the neural plate is present but fails to initiate neural tube closure
• during epithelial to mesenchymal transition in the primitive streak breakdown of the basement membrane appears somewhat disrupted with large laminin aggregates seen on nascent mesodermal cells
• accumulation of T expressing cells in the primitive streak indicating an impairment in migration of cells away from the streak
• node cells are specified but fail to coalesce to form a normal node
• properly organized somites do not form

cellular
• cultured cells from E7.5 embryos fail to adhere to either fibronectin or Matrigel matrices
• in the mesoderm and basal regions of the epiblast at E7.5
• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half
• decrease in the mitotic index in E7.5 embryos

cardiovascular system

growth/size/body
• the embryonic region is smaller compared to control littermates from E7.5 onwards

nervous system
• the neural plate is present but fails to initiate neural tube closure




Genotype
MGI:5050120
cn4
Allelic
Composition
Rac1tm1Tyb/Rac1tm2Tyb
Tg(Ttr-cre)1Hadj/0
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6 * CBA/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm1Tyb mutation (0 available); any Rac1 mutation (24 available)
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
Tg(Ttr-cre)1Hadj mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• survive longer than germ line null mice, up to E8.25

embryo
• migration of anterior visceral endoderm cells is disrupted
• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression
• expression analysis indicates a defect in the initial specification of the AP axis in most embryos
• constriction at the boundary between embryonic and extraembryonic regions
• visceral endoderm cells fail to form a border around the midline structures

craniofacial
• abnormal headfolds at E7.5

cellular
• migration of anterior visceral endoderm cells is disrupted
• defect in migration is not as severe as in germ line null mice probably due to the timing of cre expression




Genotype
MGI:5050118
cn5
Allelic
Composition
Ptentm1Ppp/Pten+
Rac1tm1Tyb/Rac1tm2Tyb
Edil3Tg(Sox2-cre)1Amc/Edil3+
Genetic
Background
involves: 129S/Sv * C3H * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Edil3Tg(Sox2-cre)1Amc mutation (5 available); any Edil3 mutation (43 available)
Ptentm1Ppp mutation (0 available); any Pten mutation (88 available)
Rac1tm1Tyb mutation (0 available); any Rac1 mutation (24 available)
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• embryo morphology is stated to be indistinguishable from that of mutant mice wild-type for Pten

cellular
• cell death is reduced compared to mutant mice wild-type for Pten




Genotype
MGI:5050122
cx6
Allelic
Composition
Rac1tm2Tyb/Rac1tm2Tyb
Tg(Hhex-EGFP)#Rbe/0
Genetic
Background
mixed
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rac1tm2Tyb mutation (0 available); any Rac1 mutation (24 available)
Tg(Hhex-EGFP)#Rbe mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
• between E5.5 and E5.75 some cells lose contact with the basement membrane
• at E6.5 cells that fail to migrate form a multilayered epithelium

cellular
• anterior visceral endoderm cells fail to migrate from the distal tip to the embryonic-extraembryonic boundary by E6.5
• between E5.5 and E5.75 some cells lose contact with the basement membrane
• at E6.5 cells that fail to migrate form a multilayered epithelium





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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory